Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lexA and SO4604 overlap by 4 nucleotides SO4604 and SO4606 are separated by 404 nucleotides SO4606 and SO4607 are separated by 14 nucleotides
SO4603: lexA - LexA repressor (NCBI ptt file), at 4,799,182 to 4,799,802
lexA
SO4604: SO4604 - conserved hypothetical protein (NCBI ptt file), at 4,799,799 to 4,800,311
SO4604
SO4606: SO4606 - cytochrome c oxidase, subunit II (NCBI ptt file), at 4,800,716 to 4,802,257
SO4606
SO4607: SO4607 - cytochrome c oxidase, subunit I (NCBI ptt file), at 4,802,272 to 4,803,864
SO4607
Position (kb)
4800
4801
4802
4803 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 4799.721 kb on + strand, within lexA at 4799.768 kb on + strand at 4799.776 kb on - strand at 4799.776 kb on - strand at 4799.784 kb on - strand at 4799.816 kb on - strand at 4799.823 kb on - strand at 4799.823 kb on - strand at 4799.833 kb on - strand at 4799.848 kb on + strand at 4799.861 kb on - strand, within SO4604 at 4799.917 kb on - strand, within SO4604 at 4799.937 kb on - strand, within SO4604 at 4800.078 kb on + strand, within SO4604 at 4800.094 kb on - strand, within SO4604 at 4800.211 kb on + strand, within SO4604 at 4800.340 kb on - strand at 4800.536 kb on - strand at 4800.541 kb on + strand at 4800.545 kb on + strand at 4800.549 kb on - strand at 4800.601 kb on - strand at 4800.650 kb on + strand at 4800.675 kb on + strand at 4800.678 kb on - strand at 4800.735 kb on - strand at 4800.761 kb on + strand at 4800.797 kb on - strand at 4800.797 kb on - strand at 4800.832 kb on - strand at 4800.845 kb on + strand at 4800.878 kb on - strand, within SO4606 at 4801.113 kb on - strand, within SO4606 at 4801.157 kb on - strand, within SO4606 at 4801.177 kb on + strand, within SO4606 at 4801.177 kb on + strand, within SO4606 at 4801.185 kb on - strand, within SO4606 at 4801.185 kb on - strand, within SO4606 at 4801.185 kb on - strand, within SO4606 at 4801.185 kb on - strand, within SO4606 at 4801.208 kb on + strand, within SO4606 at 4801.215 kb on + strand, within SO4606 at 4801.250 kb on + strand, within SO4606 at 4801.257 kb on + strand, within SO4606 at 4801.257 kb on + strand, within SO4606 at 4801.265 kb on - strand, within SO4606 at 4801.324 kb on - strand, within SO4606 at 4801.337 kb on + strand, within SO4606 at 4801.365 kb on - strand, within SO4606 at 4801.388 kb on - strand, within SO4606 at 4801.569 kb on - strand, within SO4606 at 4801.581 kb on - strand, within SO4606 at 4801.584 kb on - strand, within SO4606 at 4801.602 kb on - strand, within SO4606 at 4801.609 kb on + strand, within SO4606 at 4801.631 kb on + strand, within SO4606 at 4801.638 kb on - strand, within SO4606 at 4801.639 kb on - strand, within SO4606 at 4801.639 kb on - strand, within SO4606 at 4801.874 kb on + strand, within SO4606 at 4801.950 kb on + strand, within SO4606 at 4802.007 kb on + strand, within SO4606 at 4802.007 kb on + strand, within SO4606 at 4802.011 kb on - strand, within SO4606 at 4802.011 kb on - strand, within SO4606 at 4802.110 kb on + strand at 4802.110 kb on + strand at 4802.110 kb on + strand at 4802.113 kb on - strand at 4802.118 kb on - strand at 4802.118 kb on - strand at 4802.164 kb on + strand at 4802.180 kb on + strand at 4802.186 kb on - strand at 4802.221 kb on + strand at 4802.255 kb on - strand at 4802.337 kb on - strand at 4802.356 kb on - strand at 4802.356 kb on - strand at 4802.387 kb on + strand at 4802.409 kb on + strand at 4802.438 kb on - strand, within SO4607 at 4802.560 kb on - strand, within SO4607 at 4802.677 kb on - strand, within SO4607 at 4802.714 kb on + strand, within SO4607 at 4802.851 kb on - strand, within SO4607 at 4802.851 kb on - strand, within SO4607 at 4802.856 kb on - strand, within SO4607 at 4803.053 kb on - strand, within SO4607 at 4803.118 kb on + strand, within SO4607 at 4803.118 kb on + strand, within SO4607 at 4803.166 kb on + strand, within SO4607 at 4803.171 kb on + strand, within SO4607 at 4803.174 kb on - strand, within SO4607 at 4803.206 kb on - strand, within SO4607 at 4803.240 kb on + strand, within SO4607 at 4803.250 kb on + strand, within SO4607
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,799,721 + lexA SO4603 0.87 -5.0 4,799,768 + -3.8 4,799,776 - -3.2 4,799,776 - -4.7 4,799,784 - -2.8 4,799,816 - -3.1 4,799,823 - +4.4 4,799,823 - +0.2 4,799,833 - +0.7 4,799,848 + -2.1 4,799,861 - SO4604 0.12 -1.1 4,799,917 - SO4604 0.23 -2.5 4,799,937 - SO4604 0.27 +1.3 4,800,078 + SO4604 0.54 +0.1 4,800,094 - SO4604 0.58 -1.1 4,800,211 + SO4604 0.80 +2.0 4,800,340 - -1.8 4,800,536 - -1.3 4,800,541 + -1.1 4,800,545 + +0.0 4,800,549 - -0.9 4,800,601 - +1.9 4,800,650 + -1.9 4,800,675 + -1.3 4,800,678 - +1.3 4,800,735 - -1.3 4,800,761 + +0.4 4,800,797 - -0.6 4,800,797 - -1.1 4,800,832 - -0.4 4,800,845 + +0.5 4,800,878 - SO4606 0.11 -1.3 4,801,113 - SO4606 0.26 -0.4 4,801,157 - SO4606 0.29 -1.7 4,801,177 + SO4606 0.30 +0.6 4,801,177 + SO4606 0.30 +1.8 4,801,185 - SO4606 0.30 -1.8 4,801,185 - SO4606 0.30 -0.9 4,801,185 - SO4606 0.30 +2.2 4,801,185 - SO4606 0.30 +0.2 4,801,208 + SO4606 0.32 -1.6 4,801,215 + SO4606 0.32 -1.3 4,801,250 + SO4606 0.35 -1.8 4,801,257 + SO4606 0.35 +1.5 4,801,257 + SO4606 0.35 -0.1 4,801,265 - SO4606 0.36 +1.1 4,801,324 - SO4606 0.39 +0.3 4,801,337 + SO4606 0.40 +1.0 4,801,365 - SO4606 0.42 +0.2 4,801,388 - SO4606 0.44 -0.9 4,801,569 - SO4606 0.55 +0.5 4,801,581 - SO4606 0.56 +0.9 4,801,584 - SO4606 0.56 +1.3 4,801,602 - SO4606 0.57 +0.2 4,801,609 + SO4606 0.58 -0.2 4,801,631 + SO4606 0.59 -0.6 4,801,638 - SO4606 0.60 +0.6 4,801,639 - SO4606 0.60 +1.6 4,801,639 - SO4606 0.60 -0.3 4,801,874 + SO4606 0.75 -1.8 4,801,950 + SO4606 0.80 -0.5 4,802,007 + SO4606 0.84 +0.5 4,802,007 + SO4606 0.84 -2.3 4,802,011 - SO4606 0.84 +0.1 4,802,011 - SO4606 0.84 +0.6 4,802,110 + -1.3 4,802,110 + -0.8 4,802,110 + +2.0 4,802,113 - -0.4 4,802,118 - -1.1 4,802,118 - -1.4 4,802,164 + +0.3 4,802,180 + +2.2 4,802,186 - +1.2 4,802,221 + -2.3 4,802,255 - +1.6 4,802,337 - -1.1 4,802,356 - -0.7 4,802,356 - +2.1 4,802,387 + +0.5 4,802,409 + -2.2 4,802,438 - SO4607 0.10 -0.7 4,802,560 - SO4607 0.18 -1.3 4,802,677 - SO4607 0.25 -0.7 4,802,714 + SO4607 0.28 -2.0 4,802,851 - SO4607 0.36 +0.8 4,802,851 - SO4607 0.36 -1.8 4,802,856 - SO4607 0.37 -1.6 4,803,053 - SO4607 0.49 +0.0 4,803,118 + SO4607 0.53 -2.2 4,803,118 + SO4607 0.53 -1.2 4,803,166 + SO4607 0.56 +2.0 4,803,171 + SO4607 0.56 -0.4 4,803,174 - SO4607 0.57 +1.6 4,803,206 - SO4607 0.59 +1.5 4,803,240 + SO4607 0.61 +1.4 4,803,250 + SO4607 0.61 -1.6
Or see this region's nucleotide sequence