Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4458 and SO4461 are separated by 637 nucleotides SO4461 and SO4462 are separated by 8 nucleotides SO4462 and SO4463 are separated by 141 nucleotides
SO4458: SO4458 - carbohydrate kinase, PfkB family (NCBI ptt file), at 4,648,354 to 4,649,193
SO4458
SO4461: SO4461 - hypothetical protein (NCBI ptt file), at 4,649,831 to 4,650,028
SO4461
SO4462: SO4462 - hypothetical protein (NCBI ptt file), at 4,650,037 to 4,650,432
SO4462
SO4463: SO4463 - prolyl 4-hydroxylase, alpha subunit domain protein (NCBI ptt file), at 4,650,574 to 4,651,548
SO4463
Position (kb)
4650
4651 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4649.051 kb on + strand, within SO4458 at 4649.052 kb on + strand, within SO4458 at 4649.083 kb on - strand, within SO4458 at 4649.085 kb on + strand, within SO4458 at 4649.085 kb on + strand, within SO4458 at 4649.093 kb on - strand, within SO4458 at 4649.094 kb on - strand, within SO4458 at 4649.151 kb on - strand at 4649.207 kb on - strand at 4649.245 kb on + strand at 4649.245 kb on + strand at 4649.578 kb on + strand at 4649.606 kb on + strand at 4649.617 kb on + strand at 4649.633 kb on - strand at 4649.638 kb on - strand at 4649.655 kb on + strand at 4649.703 kb on - strand at 4649.723 kb on - strand at 4649.762 kb on + strand at 4649.762 kb on + strand at 4649.790 kb on + strand at 4649.796 kb on + strand at 4649.796 kb on + strand at 4649.796 kb on + strand at 4649.796 kb on + strand at 4649.804 kb on - strand at 4649.808 kb on + strand at 4649.808 kb on + strand at 4649.808 kb on + strand at 4649.808 kb on + strand at 4649.808 kb on + strand at 4649.816 kb on - strand at 4649.816 kb on - strand at 4649.816 kb on - strand at 4649.816 kb on - strand at 4649.816 kb on - strand at 4649.816 kb on - strand at 4649.816 kb on - strand at 4649.839 kb on + strand at 4649.849 kb on + strand at 4649.849 kb on + strand at 4649.854 kb on + strand, within SO4461 at 4649.873 kb on + strand, within SO4461 at 4649.881 kb on - strand, within SO4461 at 4649.881 kb on - strand, within SO4461 at 4649.903 kb on - strand, within SO4461 at 4649.966 kb on + strand, within SO4461 at 4649.966 kb on + strand, within SO4461 at 4650.001 kb on - strand, within SO4461 at 4650.009 kb on - strand at 4650.047 kb on + strand at 4650.047 kb on + strand at 4650.055 kb on - strand at 4650.105 kb on + strand, within SO4462 at 4650.113 kb on - strand, within SO4462 at 4650.122 kb on - strand, within SO4462 at 4650.150 kb on - strand, within SO4462 at 4650.291 kb on + strand, within SO4462 at 4650.359 kb on - strand, within SO4462 at 4650.360 kb on + strand, within SO4462 at 4650.387 kb on + strand, within SO4462 at 4650.436 kb on + strand at 4650.436 kb on + strand at 4650.436 kb on + strand at 4650.444 kb on - strand at 4650.464 kb on + strand at 4650.464 kb on + strand at 4650.464 kb on + strand at 4650.476 kb on + strand at 4650.476 kb on + strand at 4650.476 kb on + strand at 4650.476 kb on + strand at 4650.476 kb on + strand at 4650.478 kb on - strand at 4650.484 kb on - strand at 4650.484 kb on - strand at 4650.484 kb on - strand at 4650.500 kb on - strand at 4650.536 kb on + strand at 4650.545 kb on - strand at 4650.619 kb on - strand at 4650.621 kb on + strand at 4650.654 kb on + strand at 4650.654 kb on + strand at 4650.662 kb on - strand at 4650.662 kb on - strand at 4650.667 kb on + strand at 4650.684 kb on - strand, within SO4463 at 4650.766 kb on - strand, within SO4463 at 4650.766 kb on - strand, within SO4463 at 4650.768 kb on + strand, within SO4463 at 4650.812 kb on + strand, within SO4463 at 4650.913 kb on - strand, within SO4463 at 4650.918 kb on + strand, within SO4463 at 4651.014 kb on + strand, within SO4463 at 4651.022 kb on - strand, within SO4463 at 4651.022 kb on - strand, within SO4463 at 4651.045 kb on + strand, within SO4463 at 4651.053 kb on - strand, within SO4463 at 4651.080 kb on - strand, within SO4463 at 4651.082 kb on + strand, within SO4463 at 4651.082 kb on + strand, within SO4463 at 4651.082 kb on + strand, within SO4463 at 4651.082 kb on + strand, within SO4463 at 4651.088 kb on - strand, within SO4463 at 4651.156 kb on - strand, within SO4463 at 4651.240 kb on + strand, within SO4463 at 4651.367 kb on - strand, within SO4463
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,649,051 + SO4458 0.83 -1.3 4,649,052 + SO4458 0.83 +1.6 4,649,083 - SO4458 0.87 +0.1 4,649,085 + SO4458 0.87 +1.4 4,649,085 + SO4458 0.87 +2.0 4,649,093 - SO4458 0.88 -0.3 4,649,094 - SO4458 0.88 +2.5 4,649,151 - +0.8 4,649,207 - +1.4 4,649,245 + +0.5 4,649,245 + -0.1 4,649,578 + -1.5 4,649,606 + -1.7 4,649,617 + -1.5 4,649,633 - -0.5 4,649,638 - +0.5 4,649,655 + -1.8 4,649,703 - -0.6 4,649,723 - -0.4 4,649,762 + -2.0 4,649,762 + +0.6 4,649,790 + +1.9 4,649,796 + -0.4 4,649,796 + +0.0 4,649,796 + -0.2 4,649,796 + -2.9 4,649,804 - +1.0 4,649,808 + -1.9 4,649,808 + -2.3 4,649,808 + -2.5 4,649,808 + -0.0 4,649,808 + +0.2 4,649,816 - +1.2 4,649,816 - +0.5 4,649,816 - -0.1 4,649,816 - -2.0 4,649,816 - -2.3 4,649,816 - +0.5 4,649,816 - +0.7 4,649,839 + -0.5 4,649,849 + +1.3 4,649,849 + -1.5 4,649,854 + SO4461 0.12 +0.9 4,649,873 + SO4461 0.21 +0.8 4,649,881 - SO4461 0.25 -0.5 4,649,881 - SO4461 0.25 +1.6 4,649,903 - SO4461 0.36 +2.1 4,649,966 + SO4461 0.68 +0.3 4,649,966 + SO4461 0.68 +0.1 4,650,001 - SO4461 0.86 -1.8 4,650,009 - -1.2 4,650,047 + -4.7 4,650,047 + -3.1 4,650,055 - -5.0 4,650,105 + SO4462 0.17 -4.4 4,650,113 - SO4462 0.19 -4.8 4,650,122 - SO4462 0.21 -5.1 4,650,150 - SO4462 0.29 -1.2 4,650,291 + SO4462 0.64 -2.0 4,650,359 - SO4462 0.81 -0.9 4,650,360 + SO4462 0.82 -2.6 4,650,387 + SO4462 0.88 -3.1 4,650,436 + -5.6 4,650,436 + -4.7 4,650,436 + -4.6 4,650,444 - -4.0 4,650,464 + -2.5 4,650,464 + -4.5 4,650,464 + -3.3 4,650,476 + -3.6 4,650,476 + -0.9 4,650,476 + -3.0 4,650,476 + -2.8 4,650,476 + -4.1 4,650,478 - -3.2 4,650,484 - -0.9 4,650,484 - -0.0 4,650,484 - -2.8 4,650,500 - -1.8 4,650,536 + -3.5 4,650,545 - -0.7 4,650,619 - -2.5 4,650,621 + -0.9 4,650,654 + +1.1 4,650,654 + +0.8 4,650,662 - +0.8 4,650,662 - +1.1 4,650,667 + -4.1 4,650,684 - SO4463 0.11 +1.7 4,650,766 - SO4463 0.20 +0.8 4,650,766 - SO4463 0.20 +0.4 4,650,768 + SO4463 0.20 +0.0 4,650,812 + SO4463 0.24 +3.6 4,650,913 - SO4463 0.35 -0.5 4,650,918 + SO4463 0.35 -0.5 4,651,014 + SO4463 0.45 -2.3 4,651,022 - SO4463 0.46 -1.8 4,651,022 - SO4463 0.46 -2.5 4,651,045 + SO4463 0.48 +0.9 4,651,053 - SO4463 0.49 +1.2 4,651,080 - SO4463 0.52 +0.3 4,651,082 + SO4463 0.52 -0.5 4,651,082 + SO4463 0.52 -0.4 4,651,082 + SO4463 0.52 +0.0 4,651,082 + SO4463 0.52 -0.5 4,651,088 - SO4463 0.53 -3.1 4,651,156 - SO4463 0.60 -3.8 4,651,240 + SO4463 0.68 +0.1 4,651,367 - SO4463 0.81 +0.2
Or see this region's nucleotide sequence