Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaA and SO4453 are separated by 369 nucleotides SO4453 and SO4454 are separated by 289 nucleotides
SO4452: moaA - molybdenum cofactor biosynthesis protein A (NCBI ptt file), at 4,640,134 to 4,641,114
moaA
SO4453: SO4453 - electron transfer flavoprotein-ubiquinone oxidoreductase, putative (NCBI ptt file), at 4,641,484 to 4,643,133
SO4453
SO4454: SO4454 - methyl-accepting chemotaxis protein (NCBI ptt file), at 4,643,423 to 4,645,351
SO4454
Position (kb)
4641
4642
4643
4644 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4640.488 kb on - strand, within moaA at 4640.488 kb on - strand, within moaA at 4640.577 kb on + strand, within moaA at 4640.614 kb on + strand, within moaA at 4640.639 kb on - strand, within moaA at 4640.678 kb on - strand, within moaA at 4640.747 kb on - strand, within moaA at 4640.747 kb on - strand, within moaA at 4640.760 kb on + strand, within moaA at 4640.778 kb on - strand, within moaA at 4640.778 kb on - strand, within moaA at 4640.783 kb on - strand, within moaA at 4640.811 kb on + strand, within moaA at 4641.155 kb on - strand at 4641.159 kb on + strand at 4641.189 kb on - strand at 4641.216 kb on + strand at 4641.216 kb on + strand at 4641.252 kb on + strand at 4641.268 kb on + strand at 4641.294 kb on + strand at 4641.300 kb on - strand at 4641.302 kb on - strand at 4641.371 kb on + strand at 4641.371 kb on + strand at 4641.376 kb on + strand at 4641.433 kb on + strand at 4641.435 kb on + strand at 4641.435 kb on + strand at 4641.477 kb on - strand at 4641.709 kb on + strand, within SO4453 at 4641.755 kb on - strand, within SO4453 at 4641.840 kb on + strand, within SO4453 at 4641.851 kb on + strand at 4641.874 kb on - strand, within SO4453 at 4641.918 kb on - strand, within SO4453 at 4642.170 kb on + strand, within SO4453 at 4642.170 kb on + strand, within SO4453 at 4642.219 kb on + strand, within SO4453 at 4642.317 kb on + strand, within SO4453 at 4642.317 kb on + strand, within SO4453 at 4642.326 kb on + strand, within SO4453 at 4642.380 kb on - strand, within SO4453 at 4642.380 kb on - strand, within SO4453 at 4642.472 kb on + strand, within SO4453 at 4642.531 kb on + strand, within SO4453 at 4642.628 kb on - strand, within SO4453 at 4642.660 kb on + strand, within SO4453 at 4642.698 kb on - strand, within SO4453 at 4642.728 kb on + strand, within SO4453 at 4642.761 kb on - strand, within SO4453 at 4642.933 kb on - strand, within SO4453 at 4642.959 kb on + strand, within SO4453 at 4643.007 kb on + strand at 4643.079 kb on - strand at 4643.093 kb on + strand at 4643.101 kb on - strand at 4643.101 kb on - strand at 4643.101 kb on - strand at 4643.101 kb on - strand at 4643.190 kb on - strand at 4643.288 kb on - strand at 4643.294 kb on - strand at 4643.294 kb on - strand at 4643.294 kb on - strand at 4643.342 kb on + strand at 4643.355 kb on - strand at 4643.362 kb on + strand at 4643.409 kb on + strand at 4643.418 kb on - strand at 4643.492 kb on - strand at 4643.505 kb on - strand at 4643.524 kb on - strand at 4643.591 kb on - strand at 4643.767 kb on + strand, within SO4454 at 4643.800 kb on - strand, within SO4454 at 4643.802 kb on - strand, within SO4454 at 4643.832 kb on - strand, within SO4454 at 4644.036 kb on + strand, within SO4454 at 4644.077 kb on - strand, within SO4454 at 4644.107 kb on + strand, within SO4454 at 4644.107 kb on + strand, within SO4454 at 4644.108 kb on + strand, within SO4454 at 4644.109 kb on + strand, within SO4454 at 4644.109 kb on + strand, within SO4454 at 4644.115 kb on - strand, within SO4454 at 4644.126 kb on + strand, within SO4454
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,640,488 - moaA SO4452 0.36 +2.1 4,640,488 - moaA SO4452 0.36 +1.1 4,640,577 + moaA SO4452 0.45 -1.9 4,640,614 + moaA SO4452 0.49 -2.3 4,640,639 - moaA SO4452 0.51 -0.1 4,640,678 - moaA SO4452 0.55 +3.3 4,640,747 - moaA SO4452 0.62 +1.0 4,640,747 - moaA SO4452 0.62 -0.5 4,640,760 + moaA SO4452 0.64 +1.4 4,640,778 - moaA SO4452 0.66 +0.6 4,640,778 - moaA SO4452 0.66 -1.2 4,640,783 - moaA SO4452 0.66 +0.7 4,640,811 + moaA SO4452 0.69 +0.7 4,641,155 - -0.1 4,641,159 + +3.5 4,641,189 - +2.3 4,641,216 + -1.5 4,641,216 + -0.1 4,641,252 + +1.2 4,641,268 + +1.8 4,641,294 + -0.7 4,641,300 - +0.6 4,641,302 - -0.4 4,641,371 + +0.9 4,641,371 + -0.8 4,641,376 + -1.9 4,641,433 + +1.4 4,641,435 + +0.4 4,641,435 + +0.5 4,641,477 - -3.3 4,641,709 + SO4453 0.14 -3.4 4,641,755 - SO4453 0.16 -1.9 4,641,840 + SO4453 0.22 -3.9 4,641,851 + +1.1 4,641,874 - SO4453 0.24 -0.9 4,641,918 - SO4453 0.26 -2.6 4,642,170 + SO4453 0.42 -3.3 4,642,170 + SO4453 0.42 -1.9 4,642,219 + SO4453 0.45 -2.7 4,642,317 + SO4453 0.50 -4.2 4,642,317 + SO4453 0.50 -4.0 4,642,326 + SO4453 0.51 -2.9 4,642,380 - SO4453 0.54 -3.5 4,642,380 - SO4453 0.54 -4.5 4,642,472 + SO4453 0.60 -4.1 4,642,531 + SO4453 0.63 -1.5 4,642,628 - SO4453 0.69 -2.2 4,642,660 + SO4453 0.71 -2.3 4,642,698 - SO4453 0.74 -4.9 4,642,728 + SO4453 0.75 -3.9 4,642,761 - SO4453 0.77 -0.9 4,642,933 - SO4453 0.88 -4.6 4,642,959 + SO4453 0.89 -2.2 4,643,007 + -1.7 4,643,079 - -0.9 4,643,093 + -1.5 4,643,101 - -2.6 4,643,101 - -2.8 4,643,101 - -1.9 4,643,101 - -2.2 4,643,190 - -0.9 4,643,288 - +0.7 4,643,294 - +1.7 4,643,294 - +1.0 4,643,294 - -2.1 4,643,342 + +0.9 4,643,355 - -0.4 4,643,362 + +0.6 4,643,409 + -3.4 4,643,418 - -0.9 4,643,492 - +2.0 4,643,505 - -1.3 4,643,524 - -1.2 4,643,591 - +1.1 4,643,767 + SO4454 0.18 +0.8 4,643,800 - SO4454 0.20 -0.8 4,643,802 - SO4454 0.20 +0.1 4,643,832 - SO4454 0.21 -0.1 4,644,036 + SO4454 0.32 -0.3 4,644,077 - SO4454 0.34 +1.5 4,644,107 + SO4454 0.35 -0.7 4,644,107 + SO4454 0.35 +1.3 4,644,108 + SO4454 0.36 +0.6 4,644,109 + SO4454 0.36 +1.4 4,644,109 + SO4454 0.36 -2.3 4,644,115 - SO4454 0.36 -3.5 4,644,126 + SO4454 0.36 +0.3
Or see this region's nucleotide sequence