Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4297 and SO4298 are separated by 313 nucleotides SO4298 and cat are separated by 185 nucleotides
SO4297: SO4297 - uridine phosphorylase, putative (NCBI ptt file), at 4,475,715 to 4,476,440
SO4297
SO4298: SO4298 - cyclic nucleotide binding protein, putative (NCBI ptt file), at 4,476,754 to 4,477,428
SO4298
SO4299: cat - chloramphenicol acetyltransferase (NCBI ptt file), at 4,477,614 to 4,478,246
cat
Position (kb)
4476
4477
4478 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4475.879 kb on + strand, within SO4297 at 4475.891 kb on - strand, within SO4297 at 4475.899 kb on + strand, within SO4297 at 4475.934 kb on - strand, within SO4297 at 4476.017 kb on - strand, within SO4297 at 4476.084 kb on + strand, within SO4297 at 4476.120 kb on + strand, within SO4297 at 4476.140 kb on + strand, within SO4297 at 4476.201 kb on + strand, within SO4297 at 4476.227 kb on + strand, within SO4297 at 4476.272 kb on - strand, within SO4297 at 4476.309 kb on + strand, within SO4297 at 4476.312 kb on - strand, within SO4297 at 4476.327 kb on - strand, within SO4297 at 4476.327 kb on - strand, within SO4297 at 4476.354 kb on + strand, within SO4297 at 4476.364 kb on + strand, within SO4297 at 4476.374 kb on + strand at 4476.386 kb on + strand at 4476.394 kb on - strand at 4476.451 kb on + strand at 4476.451 kb on + strand at 4476.451 kb on + strand at 4476.459 kb on - strand at 4476.530 kb on - strand at 4476.597 kb on + strand at 4476.597 kb on + strand at 4476.693 kb on + strand at 4476.726 kb on - strand at 4476.764 kb on + strand at 4476.794 kb on + strand at 4476.801 kb on - strand at 4476.802 kb on - strand at 4476.816 kb on + strand at 4476.835 kb on - strand, within SO4298 at 4476.837 kb on + strand, within SO4298 at 4476.906 kb on + strand, within SO4298 at 4476.920 kb on + strand, within SO4298 at 4477.035 kb on - strand, within SO4298 at 4477.073 kb on - strand, within SO4298 at 4477.152 kb on + strand, within SO4298 at 4477.152 kb on + strand, within SO4298 at 4477.160 kb on - strand, within SO4298 at 4477.166 kb on - strand, within SO4298 at 4477.171 kb on - strand, within SO4298 at 4477.211 kb on - strand, within SO4298 at 4477.357 kb on - strand, within SO4298 at 4477.383 kb on + strand at 4477.394 kb on + strand at 4477.395 kb on + strand at 4477.402 kb on - strand at 4477.425 kb on + strand at 4477.428 kb on + strand at 4477.436 kb on - strand at 4477.436 kb on - strand at 4477.443 kb on + strand at 4477.445 kb on + strand at 4477.448 kb on + strand at 4477.456 kb on + strand at 4477.464 kb on - strand at 4477.478 kb on + strand at 4477.485 kb on + strand at 4477.486 kb on - strand at 4477.486 kb on - strand at 4477.497 kb on + strand at 4477.505 kb on - strand at 4477.528 kb on + strand at 4477.529 kb on + strand at 4477.536 kb on - strand at 4477.536 kb on - strand at 4477.555 kb on + strand at 4477.563 kb on - strand at 4477.563 kb on - strand at 4477.563 kb on - strand at 4477.565 kb on + strand at 4477.567 kb on + strand at 4477.585 kb on - strand at 4477.651 kb on + strand at 4477.664 kb on + strand at 4477.664 kb on + strand at 4477.664 kb on + strand at 4477.672 kb on - strand at 4477.672 kb on - strand at 4477.672 kb on - strand at 4477.679 kb on + strand, within cat at 4477.679 kb on + strand, within cat at 4477.686 kb on - strand, within cat at 4477.699 kb on - strand, within cat at 4477.729 kb on + strand at 4477.729 kb on + strand, within cat at 4477.737 kb on - strand, within cat at 4477.737 kb on - strand, within cat at 4477.737 kb on - strand, within cat at 4477.737 kb on - strand, within cat at 4477.759 kb on - strand, within cat at 4477.808 kb on + strand, within cat at 4477.810 kb on + strand, within cat at 4477.851 kb on + strand, within cat at 4477.852 kb on + strand, within cat at 4477.859 kb on - strand, within cat at 4477.860 kb on - strand, within cat at 4477.926 kb on + strand, within cat at 4477.947 kb on - strand, within cat at 4477.954 kb on + strand, within cat at 4477.980 kb on - strand, within cat at 4477.996 kb on + strand, within cat at 4477.996 kb on + strand, within cat at 4477.996 kb on + strand, within cat at 4478.004 kb on - strand, within cat at 4478.005 kb on - strand, within cat at 4478.019 kb on + strand, within cat at 4478.019 kb on + strand, within cat at 4478.019 kb on + strand, within cat at 4478.027 kb on - strand, within cat at 4478.027 kb on - strand, within cat at 4478.028 kb on + strand, within cat at 4478.057 kb on + strand, within cat at 4478.057 kb on + strand, within cat at 4478.057 kb on + strand, within cat at 4478.057 kb on - strand, within cat at 4478.057 kb on - strand, within cat at 4478.058 kb on + strand, within cat at 4478.058 kb on - strand, within cat at 4478.062 kb on + strand, within cat at 4478.163 kb on - strand, within cat at 4478.177 kb on + strand, within cat at 4478.177 kb on + strand, within cat at 4478.241 kb on - strand at 4478.272 kb on + strand at 4478.300 kb on + strand at 4478.308 kb on + strand at 4478.330 kb on + strand at 4478.340 kb on + strand at 4478.352 kb on + strand at 4478.360 kb on - strand at 4478.360 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,475,879 + SO4297 0.23 -2.4 4,475,891 - SO4297 0.24 +0.6 4,475,899 + SO4297 0.25 -1.3 4,475,934 - SO4297 0.30 +0.4 4,476,017 - SO4297 0.42 -1.8 4,476,084 + SO4297 0.51 +0.5 4,476,120 + SO4297 0.56 +2.1 4,476,140 + SO4297 0.59 +1.5 4,476,201 + SO4297 0.67 +0.2 4,476,227 + SO4297 0.71 -2.8 4,476,272 - SO4297 0.77 +2.0 4,476,309 + SO4297 0.82 +0.5 4,476,312 - SO4297 0.82 -2.7 4,476,327 - SO4297 0.84 -0.2 4,476,327 - SO4297 0.84 -2.5 4,476,354 + SO4297 0.88 -2.4 4,476,364 + SO4297 0.89 -3.0 4,476,374 + +0.6 4,476,386 + +0.3 4,476,394 - +1.1 4,476,451 + +0.6 4,476,451 + +1.2 4,476,451 + -2.5 4,476,459 - +1.1 4,476,530 - +0.4 4,476,597 + -1.3 4,476,597 + +0.2 4,476,693 + +1.2 4,476,726 - -0.6 4,476,764 + +0.6 4,476,794 + +0.6 4,476,801 - +0.9 4,476,802 - +0.6 4,476,816 + +0.3 4,476,835 - SO4298 0.12 -1.5 4,476,837 + SO4298 0.12 +0.4 4,476,906 + SO4298 0.23 +1.1 4,476,920 + SO4298 0.25 -0.2 4,477,035 - SO4298 0.42 -1.0 4,477,073 - SO4298 0.47 -0.1 4,477,152 + SO4298 0.59 +0.2 4,477,152 + SO4298 0.59 +0.4 4,477,160 - SO4298 0.60 +0.3 4,477,166 - SO4298 0.61 -2.6 4,477,171 - SO4298 0.62 -0.2 4,477,211 - SO4298 0.68 -2.7 4,477,357 - SO4298 0.89 -1.9 4,477,383 + -0.6 4,477,394 + +0.2 4,477,395 + +0.2 4,477,402 - -0.3 4,477,425 + +1.1 4,477,428 + +0.1 4,477,436 - -1.6 4,477,436 - +0.6 4,477,443 + +0.2 4,477,445 + -2.1 4,477,448 + +0.4 4,477,456 + -0.7 4,477,464 - +1.4 4,477,478 + +0.8 4,477,485 + -1.5 4,477,486 - +0.7 4,477,486 - -0.3 4,477,497 + +0.9 4,477,505 - +0.1 4,477,528 + +2.1 4,477,529 + +2.5 4,477,536 - -0.5 4,477,536 - -0.3 4,477,555 + +0.2 4,477,563 - -2.8 4,477,563 - +1.4 4,477,563 - -0.2 4,477,565 + +2.8 4,477,567 + -1.5 4,477,585 - +0.8 4,477,651 + -3.9 4,477,664 + -0.6 4,477,664 + -1.1 4,477,664 + +0.2 4,477,672 - +0.2 4,477,672 - +3.1 4,477,672 - -0.9 4,477,679 + cat SO4299 0.10 +1.2 4,477,679 + cat SO4299 0.10 +3.1 4,477,686 - cat SO4299 0.11 -2.9 4,477,699 - cat SO4299 0.13 -2.4 4,477,729 + +1.1 4,477,729 + cat SO4299 0.18 -1.5 4,477,737 - cat SO4299 0.19 +0.8 4,477,737 - cat SO4299 0.19 -2.2 4,477,737 - cat SO4299 0.19 +0.1 4,477,737 - cat SO4299 0.19 +1.0 4,477,759 - cat SO4299 0.23 +0.8 4,477,808 + cat SO4299 0.31 -0.7 4,477,810 + cat SO4299 0.31 -0.8 4,477,851 + cat SO4299 0.37 -1.4 4,477,852 + cat SO4299 0.38 +1.7 4,477,859 - cat SO4299 0.39 -0.7 4,477,860 - cat SO4299 0.39 +0.3 4,477,926 + cat SO4299 0.49 +0.8 4,477,947 - cat SO4299 0.53 +1.3 4,477,954 + cat SO4299 0.54 -1.3 4,477,980 - cat SO4299 0.58 +0.1 4,477,996 + cat SO4299 0.60 +2.4 4,477,996 + cat SO4299 0.60 -0.3 4,477,996 + cat SO4299 0.60 -4.1 4,478,004 - cat SO4299 0.62 +3.7 4,478,005 - cat SO4299 0.62 +0.2 4,478,019 + cat SO4299 0.64 -1.3 4,478,019 + cat SO4299 0.64 +0.1 4,478,019 + cat SO4299 0.64 -1.9 4,478,027 - cat SO4299 0.65 +1.1 4,478,027 - cat SO4299 0.65 +0.8 4,478,028 + cat SO4299 0.65 +1.0 4,478,057 + cat SO4299 0.70 +2.6 4,478,057 + cat SO4299 0.70 -0.6 4,478,057 + cat SO4299 0.70 +1.0 4,478,057 - cat SO4299 0.70 +1.1 4,478,057 - cat SO4299 0.70 -0.6 4,478,058 + cat SO4299 0.70 +0.9 4,478,058 - cat SO4299 0.70 -0.6 4,478,062 + cat SO4299 0.71 -1.3 4,478,163 - cat SO4299 0.87 -2.1 4,478,177 + cat SO4299 0.89 -0.9 4,478,177 + cat SO4299 0.89 -1.1 4,478,241 - +1.3 4,478,272 + -0.8 4,478,300 + -0.5 4,478,308 + -1.9 4,478,330 + -0.5 4,478,340 + -0.0 4,478,352 + +0.4 4,478,360 - -0.7 4,478,360 - -0.6
Or see this region's nucleotide sequence