Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4239 and SO4240 are separated by 178 nucleotides SO4240 and recQ overlap by 19 nucleotides
SO4239: SO4239 - ISSod3, transposase (NCBI ptt file), at 4,416,254 to 4,417,468
SO4239
SO4240: SO4240 - hypothetical protein (NCBI ptt file), at 4,417,647 to 4,418,807
SO4240
SO4241: recQ - ATP-dependent DNA helicase RecQ (NCBI ptt file), at 4,418,789 to 4,420,612
recQ
Position (kb)
4417
4418
4419 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4417.586 kb on + strand at 4417.649 kb on + strand at 4417.649 kb on + strand at 4417.660 kb on + strand at 4417.678 kb on - strand at 4417.720 kb on - strand at 4417.736 kb on + strand at 4417.736 kb on + strand at 4417.736 kb on + strand at 4417.744 kb on - strand at 4417.744 kb on - strand at 4417.744 kb on - strand at 4417.744 kb on - strand at 4417.744 kb on - strand at 4417.820 kb on + strand, within SO4240 at 4417.884 kb on + strand, within SO4240 at 4417.909 kb on + strand, within SO4240 at 4417.917 kb on - strand, within SO4240 at 4417.985 kb on - strand, within SO4240 at 4418.006 kb on - strand, within SO4240 at 4418.020 kb on + strand, within SO4240 at 4418.333 kb on - strand, within SO4240 at 4418.333 kb on - strand, within SO4240 at 4418.410 kb on - strand, within SO4240 at 4418.440 kb on + strand, within SO4240 at 4418.440 kb on + strand, within SO4240 at 4418.448 kb on - strand, within SO4240 at 4418.597 kb on + strand, within SO4240 at 4418.597 kb on + strand, within SO4240 at 4418.597 kb on + strand, within SO4240 at 4418.605 kb on - strand, within SO4240 at 4418.605 kb on - strand, within SO4240 at 4418.605 kb on - strand, within SO4240 at 4418.667 kb on + strand, within SO4240 at 4418.676 kb on - strand, within SO4240 at 4418.986 kb on + strand, within recQ at 4418.988 kb on + strand, within recQ at 4418.988 kb on + strand, within recQ at 4418.988 kb on + strand, within recQ at 4418.988 kb on + strand, within recQ at 4418.988 kb on + strand, within recQ at 4418.996 kb on - strand, within recQ at 4418.996 kb on - strand, within recQ at 4418.996 kb on - strand, within recQ at 4418.996 kb on - strand, within recQ at 4419.118 kb on + strand, within recQ at 4419.121 kb on + strand, within recQ at 4419.130 kb on + strand, within recQ at 4419.130 kb on + strand, within recQ at 4419.130 kb on + strand, within recQ at 4419.161 kb on + strand, within recQ at 4419.246 kb on + strand, within recQ at 4419.397 kb on - strand, within recQ at 4419.438 kb on - strand, within recQ at 4419.479 kb on + strand, within recQ at 4419.487 kb on - strand, within recQ at 4419.546 kb on + strand, within recQ at 4419.546 kb on + strand, within recQ at 4419.554 kb on - strand, within recQ at 4419.601 kb on + strand, within recQ at 4419.601 kb on + strand, within recQ at 4419.601 kb on + strand, within recQ at 4419.601 kb on + strand, within recQ at 4419.601 kb on + strand, within recQ at 4419.609 kb on - strand, within recQ at 4419.609 kb on - strand, within recQ at 4419.679 kb on - strand, within recQ at 4419.731 kb on + strand, within recQ at 4419.773 kb on + strand, within recQ at 4419.776 kb on + strand, within recQ
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,417,586 + +1.4 4,417,649 + -0.3 4,417,649 + -0.0 4,417,660 + +0.7 4,417,678 - -3.4 4,417,720 - +1.1 4,417,736 + +0.6 4,417,736 + -2.5 4,417,736 + +3.1 4,417,744 - -1.2 4,417,744 - -0.3 4,417,744 - +0.0 4,417,744 - +0.6 4,417,744 - -0.7 4,417,820 + SO4240 0.15 -0.3 4,417,884 + SO4240 0.20 +0.8 4,417,909 + SO4240 0.23 -0.6 4,417,917 - SO4240 0.23 -2.0 4,417,985 - SO4240 0.29 -1.8 4,418,006 - SO4240 0.31 +0.5 4,418,020 + SO4240 0.32 +0.6 4,418,333 - SO4240 0.59 -0.3 4,418,333 - SO4240 0.59 +0.3 4,418,410 - SO4240 0.66 +0.6 4,418,440 + SO4240 0.68 -0.6 4,418,440 + SO4240 0.68 -1.5 4,418,448 - SO4240 0.69 +0.9 4,418,597 + SO4240 0.82 -0.2 4,418,597 + SO4240 0.82 -1.6 4,418,597 + SO4240 0.82 +0.4 4,418,605 - SO4240 0.83 -0.8 4,418,605 - SO4240 0.83 -0.3 4,418,605 - SO4240 0.83 -2.0 4,418,667 + SO4240 0.88 +1.1 4,418,676 - SO4240 0.89 +0.6 4,418,986 + recQ SO4241 0.11 +1.2 4,418,988 + recQ SO4241 0.11 -0.4 4,418,988 + recQ SO4241 0.11 -0.9 4,418,988 + recQ SO4241 0.11 +1.5 4,418,988 + recQ SO4241 0.11 -0.4 4,418,988 + recQ SO4241 0.11 +1.2 4,418,996 - recQ SO4241 0.11 +2.0 4,418,996 - recQ SO4241 0.11 -1.6 4,418,996 - recQ SO4241 0.11 +0.5 4,418,996 - recQ SO4241 0.11 -1.6 4,419,118 + recQ SO4241 0.18 -0.5 4,419,121 + recQ SO4241 0.18 -3.3 4,419,130 + recQ SO4241 0.19 +0.2 4,419,130 + recQ SO4241 0.19 +1.3 4,419,130 + recQ SO4241 0.19 -0.1 4,419,161 + recQ SO4241 0.20 -2.0 4,419,246 + recQ SO4241 0.25 +0.9 4,419,397 - recQ SO4241 0.33 +0.5 4,419,438 - recQ SO4241 0.36 -1.0 4,419,479 + recQ SO4241 0.38 +0.5 4,419,487 - recQ SO4241 0.38 -1.5 4,419,546 + recQ SO4241 0.42 +0.6 4,419,546 + recQ SO4241 0.42 +0.7 4,419,554 - recQ SO4241 0.42 +0.3 4,419,601 + recQ SO4241 0.45 +0.0 4,419,601 + recQ SO4241 0.45 -2.4 4,419,601 + recQ SO4241 0.45 -2.3 4,419,601 + recQ SO4241 0.45 -0.3 4,419,601 + recQ SO4241 0.45 -1.0 4,419,609 - recQ SO4241 0.45 +2.3 4,419,609 - recQ SO4241 0.45 -2.4 4,419,679 - recQ SO4241 0.49 -1.6 4,419,731 + recQ SO4241 0.52 +1.4 4,419,773 + recQ SO4241 0.54 -2.2 4,419,776 + recQ SO4241 0.54 -0.7
Or see this region's nucleotide sequence