Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4229 and glpK are separated by 88 nucleotides glpK and SO4230.1 are separated by 286 nucleotides
SO4229: SO4229 - hypothetical protein (NCBI ptt file), at 4,405,046 to 4,405,363
SO4229
SO4230: glpK - glycerol kinase (NCBI ptt file), at 4,405,452 to 4,406,936
glpK
SO4230.1: SO4230.1 - hypothetical quinone reductase (NCBI ptt file), at 4,407,223 to 4,408,188
SO4230.1
Position (kb)
4405
4406
4407 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4404.791 kb on + strand at 4404.797 kb on - strand at 4404.830 kb on - strand at 4404.857 kb on + strand at 4404.865 kb on - strand at 4404.936 kb on + strand at 4404.936 kb on + strand at 4405.062 kb on + strand at 4405.126 kb on + strand, within SO4229 at 4405.191 kb on + strand, within SO4229 at 4405.289 kb on - strand, within SO4229 at 4405.304 kb on + strand, within SO4229 at 4405.378 kb on - strand at 4405.389 kb on - strand at 4405.489 kb on + strand at 4405.489 kb on + strand at 4405.497 kb on - strand at 4405.497 kb on - strand at 4405.549 kb on + strand at 4405.573 kb on - strand at 4405.627 kb on - strand, within glpK at 4405.639 kb on - strand, within glpK at 4405.639 kb on - strand, within glpK at 4405.742 kb on - strand, within glpK at 4405.983 kb on - strand, within glpK at 4405.984 kb on - strand, within glpK at 4406.105 kb on - strand, within glpK at 4406.112 kb on - strand, within glpK at 4406.163 kb on + strand, within glpK at 4406.173 kb on + strand, within glpK at 4406.173 kb on + strand, within glpK at 4406.173 kb on + strand, within glpK at 4406.181 kb on - strand, within glpK at 4406.251 kb on - strand, within glpK at 4406.251 kb on - strand, within glpK at 4406.262 kb on + strand, within glpK at 4406.264 kb on + strand, within glpK at 4406.270 kb on - strand, within glpK at 4406.270 kb on - strand, within glpK at 4406.328 kb on - strand, within glpK at 4406.328 kb on - strand, within glpK at 4406.342 kb on + strand, within glpK at 4406.388 kb on - strand, within glpK at 4406.445 kb on - strand, within glpK at 4406.587 kb on + strand, within glpK at 4406.602 kb on - strand, within glpK at 4406.614 kb on - strand, within glpK at 4406.885 kb on + strand at 4406.885 kb on + strand at 4406.893 kb on - strand at 4406.893 kb on - strand at 4406.903 kb on - strand at 4406.904 kb on - strand at 4406.969 kb on - strand at 4407.043 kb on - strand at 4407.050 kb on + strand at 4407.050 kb on + strand at 4407.055 kb on + strand at 4407.058 kb on - strand at 4407.058 kb on - strand at 4407.175 kb on + strand at 4407.250 kb on + strand at 4407.250 kb on + strand at 4407.258 kb on - strand at 4407.358 kb on - strand, within SO4230.1 at 4407.380 kb on - strand, within SO4230.1 at 4407.385 kb on + strand, within SO4230.1 at 4407.393 kb on - strand, within SO4230.1 at 4407.393 kb on - strand, within SO4230.1 at 4407.397 kb on + strand, within SO4230.1 at 4407.405 kb on - strand, within SO4230.1 at 4407.452 kb on + strand, within SO4230.1 at 4407.461 kb on + strand, within SO4230.1 at 4407.488 kb on + strand, within SO4230.1 at 4407.547 kb on - strand, within SO4230.1 at 4407.670 kb on - strand, within SO4230.1 at 4407.717 kb on - strand, within SO4230.1 at 4407.729 kb on - strand, within SO4230.1 at 4407.730 kb on + strand, within SO4230.1 at 4407.772 kb on + strand, within SO4230.1 at 4407.798 kb on - strand, within SO4230.1 at 4407.803 kb on - strand, within SO4230.1 at 4407.824 kb on + strand, within SO4230.1 at 4407.867 kb on + strand, within SO4230.1 at 4407.916 kb on + strand, within SO4230.1 at 4407.916 kb on + strand, within SO4230.1 at 4407.916 kb on + strand, within SO4230.1 at 4407.921 kb on + strand, within SO4230.1 at 4407.923 kb on - strand, within SO4230.1 at 4407.924 kb on - strand, within SO4230.1
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,404,791 + -0.5 4,404,797 - +0.0 4,404,830 - -1.5 4,404,857 + +0.3 4,404,865 - +0.2 4,404,936 + +2.6 4,404,936 + +0.6 4,405,062 + +1.8 4,405,126 + SO4229 0.25 +0.7 4,405,191 + SO4229 0.46 +2.0 4,405,289 - SO4229 0.76 +0.4 4,405,304 + SO4229 0.81 +0.2 4,405,378 - +0.3 4,405,389 - -0.4 4,405,489 + -0.3 4,405,489 + +1.1 4,405,497 - +1.5 4,405,497 - +0.1 4,405,549 + +1.8 4,405,573 - +0.4 4,405,627 - glpK SO4230 0.12 +1.0 4,405,639 - glpK SO4230 0.13 +2.5 4,405,639 - glpK SO4230 0.13 +0.9 4,405,742 - glpK SO4230 0.20 +0.5 4,405,983 - glpK SO4230 0.36 -0.8 4,405,984 - glpK SO4230 0.36 +0.1 4,406,105 - glpK SO4230 0.44 +0.4 4,406,112 - glpK SO4230 0.44 -0.8 4,406,163 + glpK SO4230 0.48 -2.0 4,406,173 + glpK SO4230 0.49 -0.1 4,406,173 + glpK SO4230 0.49 +0.8 4,406,173 + glpK SO4230 0.49 +2.8 4,406,181 - glpK SO4230 0.49 +1.0 4,406,251 - glpK SO4230 0.54 -1.0 4,406,251 - glpK SO4230 0.54 +1.3 4,406,262 + glpK SO4230 0.55 -0.4 4,406,264 + glpK SO4230 0.55 +0.0 4,406,270 - glpK SO4230 0.55 +1.6 4,406,270 - glpK SO4230 0.55 +1.0 4,406,328 - glpK SO4230 0.59 -2.3 4,406,328 - glpK SO4230 0.59 +1.0 4,406,342 + glpK SO4230 0.60 +1.4 4,406,388 - glpK SO4230 0.63 +2.1 4,406,445 - glpK SO4230 0.67 -0.1 4,406,587 + glpK SO4230 0.76 -0.1 4,406,602 - glpK SO4230 0.77 +2.0 4,406,614 - glpK SO4230 0.78 +0.6 4,406,885 + -1.6 4,406,885 + -1.3 4,406,893 - +1.0 4,406,893 - -0.1 4,406,903 - +2.2 4,406,904 - +2.8 4,406,969 - -3.0 4,407,043 - -1.5 4,407,050 + -0.2 4,407,050 + +2.0 4,407,055 + -0.5 4,407,058 - +0.6 4,407,058 - -1.0 4,407,175 + -1.1 4,407,250 + -1.3 4,407,250 + +3.0 4,407,258 - -1.0 4,407,358 - SO4230.1 0.14 +0.7 4,407,380 - SO4230.1 0.16 +1.5 4,407,385 + SO4230.1 0.17 +0.4 4,407,393 - SO4230.1 0.18 -2.1 4,407,393 - SO4230.1 0.18 -1.0 4,407,397 + SO4230.1 0.18 +0.8 4,407,405 - SO4230.1 0.19 -0.7 4,407,452 + SO4230.1 0.24 +2.3 4,407,461 + SO4230.1 0.25 +2.0 4,407,488 + SO4230.1 0.27 +1.1 4,407,547 - SO4230.1 0.34 +1.0 4,407,670 - SO4230.1 0.46 +0.6 4,407,717 - SO4230.1 0.51 -0.3 4,407,729 - SO4230.1 0.52 +1.4 4,407,730 + SO4230.1 0.52 +1.4 4,407,772 + SO4230.1 0.57 +0.6 4,407,798 - SO4230.1 0.60 -0.0 4,407,803 - SO4230.1 0.60 -2.8 4,407,824 + SO4230.1 0.62 +0.0 4,407,867 + SO4230.1 0.67 -0.7 4,407,916 + SO4230.1 0.72 +0.6 4,407,916 + SO4230.1 0.72 +3.1 4,407,916 + SO4230.1 0.72 -1.4 4,407,921 + SO4230.1 0.72 -2.8 4,407,923 - SO4230.1 0.72 +1.0 4,407,924 - SO4230.1 0.73 +0.4
Or see this region's nucleotide sequence