Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4160 and hslV are separated by 253 nucleotides hslV and hslU are separated by 18 nucleotides hslU and SO4164 are separated by 145 nucleotides
SO4160: SO4160 - flagellar biosynthesis protein, putative (NCBI ptt file), at 4,333,899 to 4,335,077
SO4160
SO4162: hslV - ATP-dependent protease HslV (NCBI ptt file), at 4,335,331 to 4,335,855
hslV
SO4163: hslU - heat shock protein HslVU, ATPase subunit HslU (NCBI ptt file), at 4,335,874 to 4,337,196
hslU
SO4164: SO4164 - predicted FeS cluster maintenance protein (from data) , at 4,337,342 to 4,337,728
SO4164
Position (kb)
4335
4336
4337
4338 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4334.960 kb on + strand at 4334.960 kb on + strand at 4334.961 kb on - strand at 4335.009 kb on + strand at 4335.010 kb on - strand at 4335.017 kb on - strand at 4335.024 kb on - strand at 4335.059 kb on - strand at 4335.059 kb on - strand at 4335.093 kb on - strand at 4335.143 kb on + strand at 4335.143 kb on + strand at 4335.143 kb on + strand at 4335.143 kb on + strand at 4335.151 kb on - strand at 4335.234 kb on - strand at 4335.310 kb on + strand at 4335.310 kb on + strand at 4335.385 kb on + strand, within hslV at 4335.393 kb on - strand, within hslV at 4335.407 kb on - strand, within hslV at 4335.450 kb on + strand, within hslV at 4335.565 kb on - strand, within hslV at 4335.706 kb on + strand, within hslV at 4335.931 kb on - strand at 4335.974 kb on - strand at 4336.243 kb on + strand, within hslU at 4336.243 kb on + strand, within hslU at 4336.459 kb on + strand, within hslU at 4336.534 kb on - strand, within hslU at 4336.665 kb on - strand, within hslU at 4336.691 kb on + strand, within hslU at 4336.744 kb on - strand, within hslU at 4337.181 kb on - strand at 4337.181 kb on - strand at 4337.259 kb on + strand at 4337.265 kb on + strand at 4337.265 kb on + strand at 4337.265 kb on + strand at 4337.265 kb on + strand at 4337.265 kb on + strand at 4337.265 kb on + strand at 4337.265 kb on + strand at 4337.265 kb on + strand at 4337.265 kb on + strand at 4337.267 kb on - strand at 4337.273 kb on - strand at 4337.273 kb on - strand at 4337.273 kb on - strand at 4337.273 kb on - strand at 4337.467 kb on - strand, within SO4164 at 4337.477 kb on - strand, within SO4164 at 4337.488 kb on + strand, within SO4164 at 4337.496 kb on - strand, within SO4164 at 4337.594 kb on + strand, within SO4164 at 4337.594 kb on + strand, within SO4164 at 4337.664 kb on - strand, within SO4164 at 4337.690 kb on + strand at 4337.704 kb on - strand at 4337.704 kb on - strand at 4338.086 kb on - strand at 4338.090 kb on + strand at 4338.098 kb on - strand at 4338.124 kb on + strand at 4338.179 kb on + strand at 4338.179 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,334,960 + +1.3 4,334,960 + +0.0 4,334,961 - +1.5 4,335,009 + +0.1 4,335,010 - +1.3 4,335,017 - -0.6 4,335,024 - +0.0 4,335,059 - +0.7 4,335,059 - +0.1 4,335,093 - -0.1 4,335,143 + +0.5 4,335,143 + -0.2 4,335,143 + +1.0 4,335,143 + +0.0 4,335,151 - -0.0 4,335,234 - -1.0 4,335,310 + +1.4 4,335,310 + -0.6 4,335,385 + hslV SO4162 0.10 -2.6 4,335,393 - hslV SO4162 0.12 -0.1 4,335,407 - hslV SO4162 0.14 -0.8 4,335,450 + hslV SO4162 0.23 +0.1 4,335,565 - hslV SO4162 0.45 +0.2 4,335,706 + hslV SO4162 0.71 +0.7 4,335,931 - +3.9 4,335,974 - +2.7 4,336,243 + hslU SO4163 0.28 +1.5 4,336,243 + hslU SO4163 0.28 +2.9 4,336,459 + hslU SO4163 0.44 +0.9 4,336,534 - hslU SO4163 0.50 +0.8 4,336,665 - hslU SO4163 0.60 +2.0 4,336,691 + hslU SO4163 0.62 +1.7 4,336,744 - hslU SO4163 0.66 +1.8 4,337,181 - +0.0 4,337,181 - +2.0 4,337,259 + +0.9 4,337,265 + +2.1 4,337,265 + +0.7 4,337,265 + +1.0 4,337,265 + +0.5 4,337,265 + -1.0 4,337,265 + -0.2 4,337,265 + -0.4 4,337,265 + -1.3 4,337,265 + +0.5 4,337,267 - -0.3 4,337,273 - -0.1 4,337,273 - +1.8 4,337,273 - +1.5 4,337,273 - -2.3 4,337,467 - SO4164 0.32 -0.5 4,337,477 - SO4164 0.35 -0.4 4,337,488 + SO4164 0.38 -0.2 4,337,496 - SO4164 0.40 +0.8 4,337,594 + SO4164 0.65 -0.1 4,337,594 + SO4164 0.65 +0.1 4,337,664 - SO4164 0.83 +0.9 4,337,690 + +1.2 4,337,704 - -0.6 4,337,704 - +1.2 4,338,086 - -0.4 4,338,090 + +0.2 4,338,098 - -2.1 4,338,124 + +0.2 4,338,179 + -2.1 4,338,179 + +0.7
Or see this region's nucleotide sequence