Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3978 and SO3979 are separated by 332 nucleotides SO3979 and SO3980 are separated by 165 nucleotides SO3980 and narQ are separated by 278 nucleotides
SO3978: SO3978 - hypothetical EAL and a GGDEF domain protein (NCBI ptt file), at 4,114,642 to 4,115,562
SO3978
SO3979: SO3979 - conserved domain protein (NCBI ptt file), at 4,115,895 to 4,116,038
SO3979
SO3980: SO3980 - cytochrome c552 nitrite reductase (NCBI ptt file), at 4,116,204 to 4,117,607
SO3980
SO3981: narQ - nitrate/nitrite sensor protein NarQ (NCBI ptt file), at 4,117,886 to 4,119,643
narQ
Position (kb)
4116
4117
4118 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4115.301 kb on - strand, within SO3978 at 4115.317 kb on + strand, within SO3978 at 4115.344 kb on - strand, within SO3978 at 4115.345 kb on - strand, within SO3978 at 4115.482 kb on + strand at 4115.490 kb on - strand at 4115.490 kb on - strand at 4115.840 kb on + strand at 4115.852 kb on + strand at 4115.939 kb on - strand, within SO3979 at 4115.939 kb on - strand, within SO3979 at 4115.967 kb on + strand, within SO3979 at 4115.974 kb on - strand, within SO3979 at 4116.000 kb on - strand, within SO3979 at 4116.000 kb on - strand, within SO3979 at 4116.072 kb on - strand at 4116.180 kb on + strand at 4116.187 kb on - strand at 4116.188 kb on - strand at 4116.207 kb on + strand at 4116.316 kb on + strand at 4116.441 kb on + strand, within SO3980 at 4116.512 kb on - strand, within SO3980 at 4116.650 kb on + strand, within SO3980 at 4116.658 kb on - strand, within SO3980 at 4116.658 kb on - strand, within SO3980 at 4116.658 kb on - strand, within SO3980 at 4116.672 kb on + strand, within SO3980 at 4116.732 kb on - strand, within SO3980 at 4116.787 kb on - strand, within SO3980 at 4116.871 kb on + strand, within SO3980 at 4116.871 kb on + strand, within SO3980 at 4116.871 kb on + strand, within SO3980 at 4116.910 kb on - strand, within SO3980 at 4117.067 kb on - strand, within SO3980 at 4117.096 kb on + strand, within SO3980 at 4117.104 kb on - strand, within SO3980 at 4117.180 kb on + strand, within SO3980 at 4117.180 kb on + strand, within SO3980 at 4117.203 kb on - strand, within SO3980 at 4117.203 kb on - strand, within SO3980 at 4117.203 kb on - strand, within SO3980 at 4117.249 kb on - strand, within SO3980 at 4117.249 kb on - strand, within SO3980 at 4117.275 kb on - strand, within SO3980 at 4117.317 kb on + strand, within SO3980 at 4117.318 kb on + strand, within SO3980 at 4117.318 kb on + strand, within SO3980 at 4117.337 kb on + strand, within SO3980 at 4117.418 kb on + strand, within SO3980 at 4117.426 kb on - strand, within SO3980 at 4117.426 kb on - strand, within SO3980 at 4117.551 kb on - strand at 4117.667 kb on + strand at 4117.675 kb on - strand at 4117.692 kb on + strand at 4117.692 kb on + strand at 4117.714 kb on + strand at 4117.837 kb on - strand at 4117.958 kb on - strand at 4118.016 kb on - strand at 4118.085 kb on - strand, within narQ at 4118.087 kb on + strand, within narQ at 4118.098 kb on - strand, within narQ at 4118.141 kb on - strand, within narQ at 4118.181 kb on - strand, within narQ at 4118.183 kb on + strand, within narQ at 4118.191 kb on - strand, within narQ at 4118.313 kb on + strand, within narQ at 4118.326 kb on + strand, within narQ at 4118.346 kb on - strand, within narQ at 4118.380 kb on - strand, within narQ at 4118.388 kb on + strand, within narQ at 4118.454 kb on + strand, within narQ at 4118.454 kb on + strand, within narQ at 4118.473 kb on + strand, within narQ
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,115,301 - SO3978 0.72 -0.4 4,115,317 + SO3978 0.73 +1.1 4,115,344 - SO3978 0.76 -0.2 4,115,345 - SO3978 0.76 -1.1 4,115,482 + +3.1 4,115,490 - +0.9 4,115,490 - -0.1 4,115,840 + +0.7 4,115,852 + +1.1 4,115,939 - SO3979 0.31 -0.2 4,115,939 - SO3979 0.31 +2.2 4,115,967 + SO3979 0.50 -0.5 4,115,974 - SO3979 0.55 -0.7 4,116,000 - SO3979 0.73 -0.1 4,116,000 - SO3979 0.73 +0.5 4,116,072 - -1.8 4,116,180 + -0.7 4,116,187 - -2.0 4,116,188 - +0.5 4,116,207 + +1.1 4,116,316 + -1.1 4,116,441 + SO3980 0.17 -2.1 4,116,512 - SO3980 0.22 -3.2 4,116,650 + SO3980 0.32 +1.2 4,116,658 - SO3980 0.32 +3.8 4,116,658 - SO3980 0.32 +1.9 4,116,658 - SO3980 0.32 +1.2 4,116,672 + SO3980 0.33 +1.9 4,116,732 - SO3980 0.38 +1.0 4,116,787 - SO3980 0.42 +0.6 4,116,871 + SO3980 0.48 +0.9 4,116,871 + SO3980 0.48 +0.7 4,116,871 + SO3980 0.48 +1.3 4,116,910 - SO3980 0.50 +0.0 4,117,067 - SO3980 0.61 +2.1 4,117,096 + SO3980 0.64 +1.1 4,117,104 - SO3980 0.64 -1.2 4,117,180 + SO3980 0.70 -0.6 4,117,180 + SO3980 0.70 +0.3 4,117,203 - SO3980 0.71 -0.7 4,117,203 - SO3980 0.71 -1.1 4,117,203 - SO3980 0.71 +1.1 4,117,249 - SO3980 0.74 -1.2 4,117,249 - SO3980 0.74 -0.3 4,117,275 - SO3980 0.76 +1.9 4,117,317 + SO3980 0.79 -0.8 4,117,318 + SO3980 0.79 +1.6 4,117,318 + SO3980 0.79 -1.7 4,117,337 + SO3980 0.81 +1.6 4,117,418 + SO3980 0.86 -2.2 4,117,426 - SO3980 0.87 -2.2 4,117,426 - SO3980 0.87 +1.7 4,117,551 - +1.5 4,117,667 + +2.3 4,117,675 - +0.3 4,117,692 + +1.5 4,117,692 + -1.9 4,117,714 + +3.1 4,117,837 - -3.8 4,117,958 - -2.4 4,118,016 - +0.8 4,118,085 - narQ SO3981 0.11 +0.4 4,118,087 + narQ SO3981 0.11 -0.2 4,118,098 - narQ SO3981 0.12 +0.3 4,118,141 - narQ SO3981 0.15 -1.3 4,118,181 - narQ SO3981 0.17 -0.1 4,118,183 + narQ SO3981 0.17 +0.9 4,118,191 - narQ SO3981 0.17 -0.0 4,118,313 + narQ SO3981 0.24 -0.8 4,118,326 + narQ SO3981 0.25 -2.0 4,118,346 - narQ SO3981 0.26 -0.7 4,118,380 - narQ SO3981 0.28 -0.5 4,118,388 + narQ SO3981 0.29 +0.9 4,118,454 + narQ SO3981 0.32 +1.4 4,118,454 + narQ SO3981 0.32 -0.1 4,118,473 + narQ SO3981 0.33 -0.8
Or see this region's nucleotide sequence