Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hydA and hydB are separated by 12 nucleotides hydB and SO3922 are separated by 13 nucleotides SO3922 and SO3923 are separated by 69 nucleotides
SO3920: hydA - periplasmic Fe hydrogenase, large subunit (NCBI ptt file), at 4,067,806 to 4,069,038
hydA
SO3921: hydB - periplasmic Fe hydrogenase, small subunit (NCBI ptt file), at 4,069,051 to 4,069,371
hydB
SO3922: SO3922 - formate dehydrogenase, putative (NCBI ptt file), at 4,069,385 to 4,070,068
SO3922
SO3923: SO3923 - thiH protein, putative (NCBI ptt file), at 4,070,138 to 4,071,577
SO3923
Position (kb)
4069
4070
4071 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4068.411 kb on + strand, within hydA at 4068.427 kb on - strand, within hydA at 4068.464 kb on + strand, within hydA at 4068.464 kb on + strand, within hydA at 4068.464 kb on + strand, within hydA at 4068.479 kb on - strand, within hydA at 4068.714 kb on + strand, within hydA at 4068.717 kb on + strand, within hydA at 4068.725 kb on - strand, within hydA at 4068.725 kb on - strand, within hydA at 4068.732 kb on + strand, within hydA at 4068.821 kb on + strand, within hydA at 4068.821 kb on + strand, within hydA at 4068.877 kb on + strand, within hydA at 4068.885 kb on - strand, within hydA at 4069.006 kb on + strand at 4069.016 kb on + strand at 4069.208 kb on + strand, within hydB at 4069.265 kb on - strand, within hydB at 4069.544 kb on + strand, within SO3922 at 4069.544 kb on - strand, within SO3922 at 4069.544 kb on - strand, within SO3922 at 4069.790 kb on + strand, within SO3922 at 4069.806 kb on + strand, within SO3922 at 4069.814 kb on - strand, within SO3922 at 4069.862 kb on + strand, within SO3922 at 4069.885 kb on - strand, within SO3922 at 4069.892 kb on - strand, within SO3922 at 4069.953 kb on - strand, within SO3922 at 4069.973 kb on - strand, within SO3922 at 4069.976 kb on - strand, within SO3922 at 4069.994 kb on + strand, within SO3922 at 4070.001 kb on + strand at 4070.001 kb on + strand at 4070.001 kb on + strand at 4070.002 kb on - strand at 4070.068 kb on + strand at 4070.076 kb on - strand at 4070.076 kb on - strand at 4070.089 kb on + strand at 4070.153 kb on - strand at 4070.160 kb on + strand at 4070.231 kb on - strand at 4070.231 kb on - strand at 4070.240 kb on + strand at 4070.248 kb on - strand at 4070.275 kb on - strand at 4070.297 kb on - strand, within SO3923 at 4070.302 kb on - strand, within SO3923 at 4070.302 kb on - strand, within SO3923 at 4070.309 kb on - strand, within SO3923 at 4070.588 kb on - strand, within SO3923 at 4070.637 kb on + strand, within SO3923 at 4070.637 kb on + strand, within SO3923 at 4070.637 kb on + strand, within SO3923 at 4070.639 kb on - strand, within SO3923 at 4070.645 kb on - strand, within SO3923 at 4070.645 kb on - strand, within SO3923 at 4070.683 kb on - strand, within SO3923 at 4070.716 kb on + strand, within SO3923 at 4070.775 kb on + strand, within SO3923 at 4070.836 kb on + strand, within SO3923 at 4070.883 kb on + strand, within SO3923 at 4070.891 kb on - strand, within SO3923 at 4070.891 kb on - strand, within SO3923 at 4070.891 kb on - strand, within SO3923 at 4070.997 kb on - strand, within SO3923 at 4071.059 kb on - strand, within SO3923
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,068,411 + hydA SO3920 0.49 +1.4 4,068,427 - hydA SO3920 0.50 -2.3 4,068,464 + hydA SO3920 0.53 -0.1 4,068,464 + hydA SO3920 0.53 +0.3 4,068,464 + hydA SO3920 0.53 -0.4 4,068,479 - hydA SO3920 0.55 -2.0 4,068,714 + hydA SO3920 0.74 -2.7 4,068,717 + hydA SO3920 0.74 +1.5 4,068,725 - hydA SO3920 0.75 -1.1 4,068,725 - hydA SO3920 0.75 +0.4 4,068,732 + hydA SO3920 0.75 +1.3 4,068,821 + hydA SO3920 0.82 -3.2 4,068,821 + hydA SO3920 0.82 -0.6 4,068,877 + hydA SO3920 0.87 -1.7 4,068,885 - hydA SO3920 0.88 +1.5 4,069,006 + -0.8 4,069,016 + -1.1 4,069,208 + hydB SO3921 0.49 +1.7 4,069,265 - hydB SO3921 0.67 -1.7 4,069,544 + SO3922 0.23 +1.4 4,069,544 - SO3922 0.23 -1.3 4,069,544 - SO3922 0.23 +0.3 4,069,790 + SO3922 0.59 -0.2 4,069,806 + SO3922 0.62 +2.1 4,069,814 - SO3922 0.63 -1.9 4,069,862 + SO3922 0.70 +0.9 4,069,885 - SO3922 0.73 +0.9 4,069,892 - SO3922 0.74 -3.2 4,069,953 - SO3922 0.83 -1.5 4,069,973 - SO3922 0.86 -1.3 4,069,976 - SO3922 0.86 +1.2 4,069,994 + SO3922 0.89 +0.9 4,070,001 + -0.1 4,070,001 + -2.8 4,070,001 + -0.6 4,070,002 - -2.9 4,070,068 + +0.9 4,070,076 - -0.6 4,070,076 - -1.0 4,070,089 + -1.1 4,070,153 - +2.5 4,070,160 + +1.0 4,070,231 - -0.4 4,070,231 - -1.1 4,070,240 + +2.5 4,070,248 - -0.6 4,070,275 - -0.7 4,070,297 - SO3923 0.11 -1.1 4,070,302 - SO3923 0.11 -0.7 4,070,302 - SO3923 0.11 +1.0 4,070,309 - SO3923 0.12 -3.0 4,070,588 - SO3923 0.31 +2.4 4,070,637 + SO3923 0.35 +0.1 4,070,637 + SO3923 0.35 +0.6 4,070,637 + SO3923 0.35 -0.3 4,070,639 - SO3923 0.35 +1.6 4,070,645 - SO3923 0.35 +0.0 4,070,645 - SO3923 0.35 +0.8 4,070,683 - SO3923 0.38 +0.2 4,070,716 + SO3923 0.40 +0.3 4,070,775 + SO3923 0.44 -2.6 4,070,836 + SO3923 0.48 +0.5 4,070,883 + SO3923 0.52 +1.5 4,070,891 - SO3923 0.52 -1.6 4,070,891 - SO3923 0.52 -0.4 4,070,891 - SO3923 0.52 -0.3 4,070,997 - SO3923 0.60 -2.0 4,071,059 - SO3923 0.64 -0.0
Or see this region's nucleotide sequence