Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3918 and hydA are separated by 637 nucleotides hydA and hydB are separated by 12 nucleotides hydB and SO3922 are separated by 13 nucleotides
SO3918: SO3918 - conserved hypothetical protein (NCBI ptt file), at 4,064,970 to 4,067,168
SO3918
SO3920: hydA - periplasmic Fe hydrogenase, large subunit (NCBI ptt file), at 4,067,806 to 4,069,038
hydA
SO3921: hydB - periplasmic Fe hydrogenase, small subunit (NCBI ptt file), at 4,069,051 to 4,069,371
hydB
SO3922: SO3922 - formate dehydrogenase, putative (NCBI ptt file), at 4,069,385 to 4,070,068
SO3922
Position (kb)
4067
4068
4069
4070 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 4066.857 kb on + strand, within SO3918 at 4066.918 kb on - strand, within SO3918 at 4066.949 kb on + strand at 4066.992 kb on + strand at 4067.010 kb on + strand at 4067.051 kb on + strand at 4067.069 kb on - strand at 4067.076 kb on + strand at 4067.076 kb on + strand at 4067.084 kb on - strand at 4067.084 kb on - strand at 4067.084 kb on - strand at 4067.090 kb on + strand at 4067.092 kb on + strand at 4067.092 kb on + strand at 4067.092 kb on + strand at 4067.097 kb on + strand at 4067.100 kb on - strand at 4067.100 kb on - strand at 4067.100 kb on - strand at 4067.105 kb on - strand at 4067.107 kb on - strand at 4067.111 kb on + strand at 4067.111 kb on + strand at 4067.116 kb on + strand at 4067.128 kb on + strand at 4067.136 kb on - strand at 4067.141 kb on - strand at 4067.165 kb on + strand at 4067.173 kb on - strand at 4067.181 kb on - strand at 4067.202 kb on - strand at 4067.210 kb on - strand at 4067.213 kb on + strand at 4067.229 kb on - strand at 4067.229 kb on - strand at 4067.229 kb on - strand at 4067.337 kb on + strand at 4067.337 kb on + strand at 4067.337 kb on + strand at 4067.337 kb on + strand at 4067.337 kb on + strand at 4067.337 kb on + strand at 4067.337 kb on + strand at 4067.337 kb on + strand at 4067.337 kb on + strand at 4067.337 kb on + strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.345 kb on - strand at 4067.356 kb on + strand at 4067.364 kb on - strand at 4067.374 kb on - strand at 4067.379 kb on - strand at 4067.379 kb on - strand at 4067.379 kb on - strand at 4067.389 kb on - strand at 4067.390 kb on - strand at 4067.436 kb on + strand at 4067.436 kb on + strand at 4067.449 kb on + strand at 4067.471 kb on - strand at 4067.490 kb on + strand at 4067.509 kb on - strand at 4067.663 kb on + strand at 4067.684 kb on + strand at 4067.729 kb on + strand at 4067.737 kb on - strand at 4067.737 kb on - strand at 4067.796 kb on + strand at 4067.819 kb on + strand at 4067.819 kb on + strand at 4067.819 kb on + strand at 4067.819 kb on + strand at 4067.827 kb on - strand at 4067.830 kb on + strand at 4067.830 kb on + strand at 4067.838 kb on - strand at 4067.838 kb on - strand at 4067.838 kb on - strand at 4067.838 kb on - strand at 4067.838 kb on - strand at 4067.838 kb on - strand at 4067.850 kb on - strand at 4067.852 kb on + strand at 4067.852 kb on + strand at 4067.860 kb on - strand at 4067.860 kb on - strand at 4067.860 kb on - strand at 4067.860 kb on - strand at 4067.860 kb on - strand at 4067.860 kb on - strand at 4067.860 kb on - strand at 4067.860 kb on - strand at 4067.860 kb on - strand at 4067.861 kb on - strand at 4067.875 kb on + strand at 4067.875 kb on + strand at 4067.875 kb on + strand at 4067.875 kb on + strand at 4067.876 kb on + strand at 4067.876 kb on + strand at 4067.876 kb on + strand at 4067.876 kb on + strand at 4067.883 kb on - strand at 4067.883 kb on - strand at 4067.883 kb on - strand at 4067.884 kb on - strand at 4067.884 kb on - strand at 4067.884 kb on - strand at 4067.884 kb on - strand at 4067.885 kb on + strand at 4067.885 kb on + strand at 4067.890 kb on + strand at 4067.893 kb on - strand at 4067.893 kb on - strand at 4067.893 kb on - strand at 4067.898 kb on + strand at 4067.900 kb on + strand at 4067.900 kb on + strand at 4067.900 kb on + strand at 4067.908 kb on - strand at 4067.908 kb on - strand at 4067.911 kb on - strand at 4067.913 kb on - strand at 4067.913 kb on - strand at 4067.930 kb on + strand, within hydA at 4067.932 kb on + strand, within hydA at 4067.932 kb on + strand, within hydA at 4067.932 kb on + strand, within hydA at 4067.932 kb on + strand, within hydA at 4067.933 kb on + strand, within hydA at 4067.933 kb on + strand, within hydA at 4067.933 kb on + strand, within hydA at 4067.933 kb on + strand, within hydA at 4067.933 kb on - strand, within hydA at 4067.938 kb on - strand, within hydA at 4067.940 kb on - strand, within hydA at 4067.940 kb on - strand, within hydA at 4067.941 kb on - strand, within hydA at 4067.941 kb on - strand, within hydA at 4067.942 kb on + strand, within hydA at 4067.942 kb on - strand, within hydA at 4067.943 kb on - strand, within hydA at 4067.950 kb on + strand, within hydA at 4067.953 kb on + strand, within hydA at 4067.959 kb on + strand, within hydA at 4067.959 kb on + strand, within hydA at 4067.959 kb on + strand, within hydA at 4067.966 kb on + strand, within hydA at 4067.966 kb on + strand, within hydA at 4067.966 kb on + strand, within hydA at 4067.966 kb on + strand, within hydA at 4067.967 kb on - strand, within hydA at 4067.967 kb on - strand, within hydA at 4067.967 kb on - strand, within hydA at 4067.967 kb on - strand, within hydA at 4067.967 kb on - strand, within hydA at 4067.967 kb on - strand, within hydA at 4067.976 kb on + strand, within hydA at 4067.976 kb on + strand, within hydA at 4067.976 kb on + strand, within hydA at 4067.984 kb on - strand, within hydA at 4067.984 kb on - strand, within hydA at 4067.984 kb on - strand, within hydA at 4067.984 kb on - strand, within hydA at 4067.984 kb on - strand, within hydA at 4067.984 kb on - strand, within hydA at 4067.984 kb on - strand, within hydA at 4067.984 kb on - strand, within hydA at 4067.984 kb on - strand, within hydA at 4068.002 kb on + strand, within hydA at 4068.004 kb on + strand, within hydA at 4068.012 kb on + strand, within hydA at 4068.012 kb on + strand, within hydA at 4068.060 kb on - strand, within hydA at 4068.069 kb on + strand, within hydA at 4068.074 kb on + strand, within hydA at 4068.074 kb on + strand, within hydA at 4068.077 kb on - strand, within hydA at 4068.122 kb on - strand, within hydA at 4068.123 kb on + strand, within hydA at 4068.131 kb on - strand, within hydA at 4068.133 kb on - strand, within hydA at 4068.133 kb on - strand, within hydA at 4068.151 kb on + strand, within hydA at 4068.168 kb on - strand, within hydA at 4068.316 kb on + strand, within hydA at 4068.316 kb on + strand, within hydA at 4068.316 kb on + strand, within hydA at 4068.324 kb on - strand, within hydA at 4068.411 kb on + strand, within hydA at 4068.427 kb on - strand, within hydA at 4068.464 kb on + strand, within hydA at 4068.464 kb on + strand, within hydA at 4068.464 kb on + strand, within hydA at 4068.479 kb on - strand, within hydA at 4068.714 kb on + strand, within hydA at 4068.717 kb on + strand, within hydA at 4068.725 kb on - strand, within hydA at 4068.725 kb on - strand, within hydA at 4068.732 kb on + strand, within hydA at 4068.821 kb on + strand, within hydA at 4068.821 kb on + strand, within hydA at 4068.877 kb on + strand, within hydA at 4068.885 kb on - strand, within hydA at 4069.006 kb on + strand at 4069.016 kb on + strand at 4069.208 kb on + strand, within hydB at 4069.265 kb on - strand, within hydB at 4069.544 kb on + strand, within SO3922 at 4069.544 kb on - strand, within SO3922 at 4069.544 kb on - strand, within SO3922 at 4069.790 kb on + strand, within SO3922 at 4069.806 kb on + strand, within SO3922 at 4069.814 kb on - strand, within SO3922 at 4069.862 kb on + strand, within SO3922 at 4069.885 kb on - strand, within SO3922 at 4069.892 kb on - strand, within SO3922 at 4069.953 kb on - strand, within SO3922 at 4069.973 kb on - strand, within SO3922 at 4069.976 kb on - strand, within SO3922 at 4069.994 kb on + strand, within SO3922 at 4070.001 kb on + strand at 4070.001 kb on + strand at 4070.001 kb on + strand at 4070.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,066,857 + SO3918 0.86 -1.4 4,066,918 - SO3918 0.89 -0.5 4,066,949 + +0.7 4,066,992 + -0.3 4,067,010 + -1.9 4,067,051 + -0.9 4,067,069 - +0.9 4,067,076 + -0.4 4,067,076 + -2.6 4,067,084 - +1.7 4,067,084 - -0.3 4,067,084 - -0.5 4,067,090 + +1.8 4,067,092 + +0.3 4,067,092 + -0.1 4,067,092 + -1.4 4,067,097 + +2.1 4,067,100 - -0.4 4,067,100 - -2.0 4,067,100 - +2.5 4,067,105 - -1.7 4,067,107 - +0.2 4,067,111 + +0.5 4,067,111 + -1.9 4,067,116 + -2.1 4,067,128 + +0.5 4,067,136 - -0.3 4,067,141 - -0.6 4,067,165 + +0.8 4,067,173 - +1.1 4,067,181 - -1.7 4,067,202 - +0.6 4,067,210 - -1.7 4,067,213 + -0.4 4,067,229 - +0.1 4,067,229 - -2.4 4,067,229 - +0.6 4,067,337 + -1.4 4,067,337 + -1.0 4,067,337 + -1.2 4,067,337 + -0.6 4,067,337 + +0.8 4,067,337 + +1.3 4,067,337 + -1.7 4,067,337 + -3.0 4,067,337 + +0.7 4,067,337 + -1.0 4,067,345 - +0.1 4,067,345 - -0.2 4,067,345 - +0.3 4,067,345 - +1.7 4,067,345 - +2.0 4,067,345 - -0.3 4,067,345 - -2.7 4,067,345 - +1.6 4,067,345 - +0.0 4,067,345 - +2.3 4,067,345 - -0.0 4,067,345 - +0.9 4,067,345 - +2.5 4,067,345 - +0.6 4,067,345 - +1.4 4,067,345 - +2.4 4,067,356 + -0.1 4,067,364 - +0.6 4,067,374 - +0.9 4,067,379 - +1.7 4,067,379 - -0.1 4,067,379 - +0.9 4,067,389 - -2.4 4,067,390 - -0.4 4,067,436 + -1.4 4,067,436 + +1.0 4,067,449 + +1.7 4,067,471 - +1.7 4,067,490 + +0.2 4,067,509 - -0.9 4,067,663 + +1.0 4,067,684 + +1.4 4,067,729 + +2.2 4,067,737 - -0.4 4,067,737 - -1.4 4,067,796 + +0.2 4,067,819 + -0.5 4,067,819 + -0.4 4,067,819 + +0.4 4,067,819 + -0.2 4,067,827 - +2.5 4,067,830 + -0.1 4,067,830 + -1.8 4,067,838 - -0.5 4,067,838 - +2.1 4,067,838 - +0.4 4,067,838 - -0.8 4,067,838 - +0.6 4,067,838 - +1.9 4,067,850 - +0.1 4,067,852 + +1.8 4,067,852 + -2.4 4,067,860 - -1.3 4,067,860 - -1.8 4,067,860 - +0.1 4,067,860 - +1.0 4,067,860 - -0.4 4,067,860 - -0.3 4,067,860 - -4.7 4,067,860 - +0.2 4,067,860 - -2.1 4,067,861 - -0.5 4,067,875 + -0.1 4,067,875 + +0.1 4,067,875 + +2.1 4,067,875 + +0.6 4,067,876 + -0.8 4,067,876 + -1.6 4,067,876 + +1.2 4,067,876 + +0.8 4,067,883 - +0.0 4,067,883 - -0.5 4,067,883 - +1.4 4,067,884 - -1.7 4,067,884 - +0.5 4,067,884 - +0.3 4,067,884 - +1.2 4,067,885 + -1.7 4,067,885 + +0.7 4,067,890 + -1.4 4,067,893 - +0.4 4,067,893 - +2.6 4,067,893 - -0.0 4,067,898 + -0.6 4,067,900 + +1.4 4,067,900 + +0.0 4,067,900 + +0.6 4,067,908 - +1.9 4,067,908 - +0.4 4,067,911 - -1.4 4,067,913 - +0.8 4,067,913 - +1.1 4,067,930 + hydA SO3920 0.10 -1.1 4,067,932 + hydA SO3920 0.10 +0.7 4,067,932 + hydA SO3920 0.10 +0.1 4,067,932 + hydA SO3920 0.10 -0.4 4,067,932 + hydA SO3920 0.10 +2.0 4,067,933 + hydA SO3920 0.10 -1.6 4,067,933 + hydA SO3920 0.10 +0.3 4,067,933 + hydA SO3920 0.10 -1.4 4,067,933 + hydA SO3920 0.10 +0.2 4,067,933 - hydA SO3920 0.10 +0.4 4,067,938 - hydA SO3920 0.11 +1.6 4,067,940 - hydA SO3920 0.11 -1.7 4,067,940 - hydA SO3920 0.11 +0.1 4,067,941 - hydA SO3920 0.11 -0.5 4,067,941 - hydA SO3920 0.11 +0.3 4,067,942 + hydA SO3920 0.11 +2.1 4,067,942 - hydA SO3920 0.11 +0.4 4,067,943 - hydA SO3920 0.11 +0.2 4,067,950 + hydA SO3920 0.12 +1.5 4,067,953 + hydA SO3920 0.12 +5.8 4,067,959 + hydA SO3920 0.12 -2.4 4,067,959 + hydA SO3920 0.12 +1.2 4,067,959 + hydA SO3920 0.12 -0.6 4,067,966 + hydA SO3920 0.13 +0.1 4,067,966 + hydA SO3920 0.13 +1.4 4,067,966 + hydA SO3920 0.13 -0.1 4,067,966 + hydA SO3920 0.13 -2.3 4,067,967 - hydA SO3920 0.13 +2.9 4,067,967 - hydA SO3920 0.13 -2.3 4,067,967 - hydA SO3920 0.13 -1.1 4,067,967 - hydA SO3920 0.13 +2.1 4,067,967 - hydA SO3920 0.13 +1.0 4,067,967 - hydA SO3920 0.13 +0.9 4,067,976 + hydA SO3920 0.14 +0.2 4,067,976 + hydA SO3920 0.14 -1.7 4,067,976 + hydA SO3920 0.14 +0.4 4,067,984 - hydA SO3920 0.14 -0.4 4,067,984 - hydA SO3920 0.14 -0.9 4,067,984 - hydA SO3920 0.14 +0.1 4,067,984 - hydA SO3920 0.14 -1.4 4,067,984 - hydA SO3920 0.14 +0.4 4,067,984 - hydA SO3920 0.14 -2.4 4,067,984 - hydA SO3920 0.14 -0.6 4,067,984 - hydA SO3920 0.14 +1.5 4,067,984 - hydA SO3920 0.14 -0.1 4,068,002 + hydA SO3920 0.16 -0.2 4,068,004 + hydA SO3920 0.16 -1.3 4,068,012 + hydA SO3920 0.17 +1.0 4,068,012 + hydA SO3920 0.17 +0.3 4,068,060 - hydA SO3920 0.21 +0.4 4,068,069 + hydA SO3920 0.21 -1.8 4,068,074 + hydA SO3920 0.22 +0.9 4,068,074 + hydA SO3920 0.22 +1.0 4,068,077 - hydA SO3920 0.22 +0.4 4,068,122 - hydA SO3920 0.26 +0.7 4,068,123 + hydA SO3920 0.26 +1.4 4,068,131 - hydA SO3920 0.26 +0.7 4,068,133 - hydA SO3920 0.27 -1.7 4,068,133 - hydA SO3920 0.27 -1.1 4,068,151 + hydA SO3920 0.28 +0.8 4,068,168 - hydA SO3920 0.29 +2.1 4,068,316 + hydA SO3920 0.41 -1.0 4,068,316 + hydA SO3920 0.41 +1.3 4,068,316 + hydA SO3920 0.41 -1.7 4,068,324 - hydA SO3920 0.42 -1.0 4,068,411 + hydA SO3920 0.49 +1.4 4,068,427 - hydA SO3920 0.50 -2.3 4,068,464 + hydA SO3920 0.53 -0.1 4,068,464 + hydA SO3920 0.53 +0.3 4,068,464 + hydA SO3920 0.53 -0.4 4,068,479 - hydA SO3920 0.55 -2.0 4,068,714 + hydA SO3920 0.74 -2.7 4,068,717 + hydA SO3920 0.74 +1.5 4,068,725 - hydA SO3920 0.75 -1.1 4,068,725 - hydA SO3920 0.75 +0.4 4,068,732 + hydA SO3920 0.75 +1.3 4,068,821 + hydA SO3920 0.82 -3.2 4,068,821 + hydA SO3920 0.82 -0.6 4,068,877 + hydA SO3920 0.87 -1.7 4,068,885 - hydA SO3920 0.88 +1.5 4,069,006 + -0.8 4,069,016 + -1.1 4,069,208 + hydB SO3921 0.49 +1.7 4,069,265 - hydB SO3921 0.67 -1.7 4,069,544 + SO3922 0.23 +1.4 4,069,544 - SO3922 0.23 -1.3 4,069,544 - SO3922 0.23 +0.3 4,069,790 + SO3922 0.59 -0.2 4,069,806 + SO3922 0.62 +2.1 4,069,814 - SO3922 0.63 -1.9 4,069,862 + SO3922 0.70 +0.9 4,069,885 - SO3922 0.73 +0.9 4,069,892 - SO3922 0.74 -3.2 4,069,953 - SO3922 0.83 -1.5 4,069,973 - SO3922 0.86 -1.3 4,069,976 - SO3922 0.86 +1.2 4,069,994 + SO3922 0.89 +0.9 4,070,001 + -0.1 4,070,001 + -2.8 4,070,001 + -0.6 4,070,002 - -2.9
Or see this region's nucleotide sequence