Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3915 and alr are separated by 177 nucleotides alr and dnaB are separated by 76 nucleotides
SO3915: SO3915 - conserved hypothetical protein (NCBI ptt file), at 4,061,643 to 4,062,107
SO3915
SO3916: alr - alanine racemase, biosynthetic (NCBI ptt file), at 4,062,285 to 4,063,361
alr
SO3917: dnaB - replicative DNA helicase (NCBI ptt file), at 4,063,438 to 4,064,844
dnaB
Position (kb)
4062
4063
4064 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4061.450 kb on + strand at 4061.461 kb on + strand at 4061.469 kb on - strand at 4061.532 kb on + strand at 4061.540 kb on - strand at 4061.610 kb on - strand at 4061.612 kb on + strand at 4061.612 kb on + strand at 4061.614 kb on - strand at 4061.620 kb on - strand at 4061.620 kb on - strand at 4061.620 kb on - strand at 4061.627 kb on - strand at 4061.707 kb on + strand, within SO3915 at 4061.707 kb on + strand, within SO3915 at 4061.707 kb on + strand, within SO3915 at 4061.707 kb on + strand, within SO3915 at 4061.707 kb on + strand, within SO3915 at 4061.715 kb on - strand, within SO3915 at 4061.715 kb on - strand, within SO3915 at 4061.715 kb on - strand, within SO3915 at 4061.715 kb on - strand, within SO3915 at 4061.806 kb on - strand, within SO3915 at 4062.003 kb on + strand, within SO3915 at 4062.011 kb on - strand, within SO3915 at 4062.103 kb on + strand at 4062.139 kb on + strand at 4062.146 kb on - strand at 4062.147 kb on - strand at 4062.177 kb on - strand at 4062.216 kb on - strand at 4062.222 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,061,450 + -1.6 4,061,461 + -0.5 4,061,469 - -1.4 4,061,532 + -0.1 4,061,540 - +1.2 4,061,610 - +0.5 4,061,612 + +0.0 4,061,612 + +1.2 4,061,614 - -0.5 4,061,620 - -0.0 4,061,620 - +1.6 4,061,620 - +2.3 4,061,627 - +1.2 4,061,707 + SO3915 0.14 -1.0 4,061,707 + SO3915 0.14 -0.4 4,061,707 + SO3915 0.14 +2.4 4,061,707 + SO3915 0.14 +1.4 4,061,707 + SO3915 0.14 +0.1 4,061,715 - SO3915 0.15 +0.3 4,061,715 - SO3915 0.15 -0.7 4,061,715 - SO3915 0.15 +1.1 4,061,715 - SO3915 0.15 -0.5 4,061,806 - SO3915 0.35 +1.0 4,062,003 + SO3915 0.77 -0.7 4,062,011 - SO3915 0.79 +0.9 4,062,103 + -0.1 4,062,139 + +0.5 4,062,146 - +0.9 4,062,147 - -1.0 4,062,177 - -2.4 4,062,216 - -1.7 4,062,222 + -1.1
Or see this region's nucleotide sequence