Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3800 and SO3801 are separated by 104 nucleotides SO3801 and SO3802 overlap by 4 nucleotides SO3802 and hpt-2 are separated by 243 nucleotides hpt-2 and ubiX are separated by 7 nucleotides
SO3800: SO3800 - serine protease, subtilase family (NCBI ptt file), at 3,952,311 to 3,955,958
SO3800
SO3801: SO3801 - ABC transporter, permease protein (NCBI ptt file), at 3,956,063 to 3,956,833
SO3801
SO3802: SO3802 - ABC transporter, ATP-binding protein (NCBI ptt file), at 3,956,830 to 3,957,768
SO3802
SO3803: hpt-2 - hypoxanthine phosphoribosyltransferase (NCBI ptt file), at 3,958,012 to 3,958,542
hpt-2
SO3804: ubiX - phenylacrylic acid decarboxylase, 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, putative (NCBI ptt file), at 3,958,550 to 3,959,209
ubiX
Position (kb)
3956
3957
3958 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3955.865 kb on + strand at 3955.896 kb on + strand at 3955.896 kb on + strand at 3955.922 kb on - strand at 3955.927 kb on - strand at 3956.024 kb on + strand at 3956.040 kb on + strand at 3956.074 kb on + strand at 3956.074 kb on + strand at 3956.082 kb on - strand at 3956.082 kb on - strand at 3956.082 kb on - strand at 3956.109 kb on + strand at 3956.109 kb on + strand at 3956.119 kb on - strand at 3956.134 kb on + strand at 3956.142 kb on - strand, within SO3801 at 3956.166 kb on - strand, within SO3801 at 3956.273 kb on - strand, within SO3801 at 3956.288 kb on + strand, within SO3801 at 3956.288 kb on + strand, within SO3801 at 3956.375 kb on + strand, within SO3801 at 3956.375 kb on + strand, within SO3801 at 3956.383 kb on - strand, within SO3801 at 3956.416 kb on - strand, within SO3801 at 3956.473 kb on - strand, within SO3801 at 3956.519 kb on - strand, within SO3801 at 3956.544 kb on + strand, within SO3801 at 3956.545 kb on + strand, within SO3801 at 3956.552 kb on - strand, within SO3801 at 3956.562 kb on - strand, within SO3801 at 3956.562 kb on - strand, within SO3801 at 3956.589 kb on - strand, within SO3801 at 3956.658 kb on + strand, within SO3801 at 3956.734 kb on - strand, within SO3801 at 3956.760 kb on + strand at 3956.843 kb on - strand at 3956.955 kb on + strand, within SO3802 at 3956.987 kb on - strand, within SO3802 at 3956.987 kb on - strand, within SO3802 at 3957.007 kb on - strand, within SO3802 at 3957.007 kb on - strand, within SO3802 at 3957.249 kb on + strand, within SO3802 at 3957.252 kb on - strand, within SO3802 at 3957.317 kb on - strand, within SO3802 at 3957.344 kb on + strand, within SO3802 at 3957.361 kb on - strand, within SO3802 at 3957.413 kb on + strand, within SO3802 at 3957.413 kb on + strand, within SO3802 at 3957.487 kb on - strand, within SO3802 at 3957.652 kb on - strand, within SO3802 at 3957.678 kb on + strand at 3957.686 kb on - strand at 3957.828 kb on - strand at 3957.839 kb on + strand at 3957.847 kb on - strand at 3957.847 kb on - strand at 3957.943 kb on + strand at 3957.951 kb on - strand at 3957.951 kb on - strand at 3958.064 kb on + strand at 3958.113 kb on - strand, within hpt-2 at 3958.151 kb on + strand, within hpt-2 at 3958.152 kb on - strand, within hpt-2 at 3958.181 kb on + strand, within hpt-2 at 3958.321 kb on + strand, within hpt-2 at 3958.329 kb on - strand, within hpt-2 at 3958.524 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,955,865 + +0.1 3,955,896 + -0.3 3,955,896 + -0.1 3,955,922 - +0.8 3,955,927 - -0.4 3,956,024 + +1.2 3,956,040 + +0.5 3,956,074 + +0.0 3,956,074 + +4.0 3,956,082 - +1.0 3,956,082 - +1.9 3,956,082 - +3.4 3,956,109 + +2.4 3,956,109 + +1.7 3,956,119 - +1.1 3,956,134 + +1.3 3,956,142 - SO3801 0.10 +2.9 3,956,166 - SO3801 0.13 +2.2 3,956,273 - SO3801 0.27 +2.9 3,956,288 + SO3801 0.29 +2.0 3,956,288 + SO3801 0.29 +1.4 3,956,375 + SO3801 0.40 +0.7 3,956,375 + SO3801 0.40 +3.4 3,956,383 - SO3801 0.42 +1.9 3,956,416 - SO3801 0.46 +3.3 3,956,473 - SO3801 0.53 +0.3 3,956,519 - SO3801 0.59 +1.9 3,956,544 + SO3801 0.62 +1.8 3,956,545 + SO3801 0.63 +3.4 3,956,552 - SO3801 0.63 +3.4 3,956,562 - SO3801 0.65 +2.0 3,956,562 - SO3801 0.65 +1.1 3,956,589 - SO3801 0.68 +3.0 3,956,658 + SO3801 0.77 +1.6 3,956,734 - SO3801 0.87 +2.8 3,956,760 + +1.8 3,956,843 - +2.3 3,956,955 + SO3802 0.13 -0.7 3,956,987 - SO3802 0.17 +1.7 3,956,987 - SO3802 0.17 +1.5 3,957,007 - SO3802 0.19 +3.0 3,957,007 - SO3802 0.19 +2.1 3,957,249 + SO3802 0.45 +2.5 3,957,252 - SO3802 0.45 +1.0 3,957,317 - SO3802 0.52 +2.6 3,957,344 + SO3802 0.55 +3.4 3,957,361 - SO3802 0.57 -0.3 3,957,413 + SO3802 0.62 +4.6 3,957,413 + SO3802 0.62 +3.7 3,957,487 - SO3802 0.70 +0.1 3,957,652 - SO3802 0.88 +0.4 3,957,678 + +1.4 3,957,686 - +0.7 3,957,828 - -0.6 3,957,839 + +1.0 3,957,847 - +0.9 3,957,847 - -2.6 3,957,943 + -1.1 3,957,951 - -3.9 3,957,951 - -0.1 3,958,064 + -0.6 3,958,113 - hpt-2 SO3803 0.19 +0.3 3,958,151 + hpt-2 SO3803 0.26 -0.2 3,958,152 - hpt-2 SO3803 0.26 -0.6 3,958,181 + hpt-2 SO3803 0.32 +0.7 3,958,321 + hpt-2 SO3803 0.58 -0.3 3,958,329 - hpt-2 SO3803 0.60 +1.3 3,958,524 - +2.1
Or see this region's nucleotide sequence