Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3720 and SO3722 are separated by 611 nucleotides SO3722 and cysC are separated by 21 nucleotides cysC and SO3725 are separated by 172 nucleotides
SO3720: SO3720 - conserved hypothetical protein (NCBI ptt file), at 3,866,150 to 3,866,575
SO3720
SO3722: SO3722 - conserved hypothetical protein (NCBI ptt file), at 3,867,187 to 3,868,026
SO3722
SO3723: cysC - adenylylsulfate kinase (NCBI ptt file), at 3,868,048 to 3,868,665
cysC
SO3725: SO3725 - hypothetical protein (NCBI ptt file), at 3,868,838 to 3,869,161
SO3725
Position (kb)
3867
3868
3869 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3866.404 kb on + strand, within SO3720 at 3866.458 kb on - strand, within SO3720 at 3866.517 kb on + strand, within SO3720 at 3866.525 kb on - strand, within SO3720 at 3866.553 kb on + strand at 3866.617 kb on - strand at 3866.635 kb on + strand at 3866.641 kb on - strand at 3866.641 kb on - strand at 3866.641 kb on - strand at 3866.733 kb on + strand at 3866.741 kb on - strand at 3866.753 kb on - strand at 3866.769 kb on + strand at 3866.777 kb on - strand at 3866.843 kb on + strand at 3866.851 kb on - strand at 3866.917 kb on + strand at 3867.038 kb on + strand at 3867.043 kb on + strand at 3867.149 kb on - strand at 3867.152 kb on + strand at 3867.177 kb on + strand at 3867.185 kb on - strand at 3867.243 kb on - strand at 3867.272 kb on + strand, within SO3722 at 3867.280 kb on - strand, within SO3722 at 3867.416 kb on - strand, within SO3722 at 3867.554 kb on + strand, within SO3722 at 3867.554 kb on + strand, within SO3722 at 3867.554 kb on + strand, within SO3722 at 3867.554 kb on + strand, within SO3722 at 3867.562 kb on - strand, within SO3722 at 3867.562 kb on - strand, within SO3722 at 3867.562 kb on - strand, within SO3722 at 3867.569 kb on + strand, within SO3722 at 3867.569 kb on + strand, within SO3722 at 3867.569 kb on + strand, within SO3722 at 3867.569 kb on + strand, within SO3722 at 3867.577 kb on - strand, within SO3722 at 3867.577 kb on - strand, within SO3722 at 3867.577 kb on - strand, within SO3722 at 3867.577 kb on - strand, within SO3722 at 3867.577 kb on - strand, within SO3722 at 3867.594 kb on - strand, within SO3722 at 3867.622 kb on + strand, within SO3722 at 3867.622 kb on + strand, within SO3722 at 3867.725 kb on + strand, within SO3722 at 3867.731 kb on + strand, within SO3722 at 3867.739 kb on - strand, within SO3722 at 3867.739 kb on - strand, within SO3722 at 3867.739 kb on - strand, within SO3722 at 3867.759 kb on - strand, within SO3722 at 3867.764 kb on - strand, within SO3722 at 3867.986 kb on - strand at 3868.034 kb on + strand at 3868.044 kb on + strand at 3868.058 kb on + strand at 3868.173 kb on - strand, within cysC at 3868.265 kb on - strand, within cysC at 3868.346 kb on - strand, within cysC at 3868.346 kb on - strand, within cysC at 3868.354 kb on + strand, within cysC at 3868.451 kb on - strand, within cysC at 3868.639 kb on + strand at 3868.653 kb on + strand at 3868.681 kb on - strand at 3868.681 kb on - strand at 3868.817 kb on + strand at 3868.860 kb on - strand at 3868.881 kb on - strand, within SO3725 at 3869.002 kb on - strand, within SO3725 at 3869.004 kb on + strand, within SO3725
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,866,404 + SO3720 0.60 +0.6 3,866,458 - SO3720 0.72 +0.4 3,866,517 + SO3720 0.86 -1.2 3,866,525 - SO3720 0.88 +0.3 3,866,553 + +0.9 3,866,617 - -0.9 3,866,635 + +1.9 3,866,641 - +2.2 3,866,641 - +1.5 3,866,641 - +1.2 3,866,733 + +0.5 3,866,741 - +0.4 3,866,753 - +3.9 3,866,769 + +1.4 3,866,777 - -1.3 3,866,843 + +2.6 3,866,851 - +1.0 3,866,917 + -0.6 3,867,038 + -2.4 3,867,043 + -3.2 3,867,149 - -1.4 3,867,152 + +1.8 3,867,177 + +2.1 3,867,185 - +0.6 3,867,243 - +1.1 3,867,272 + SO3722 0.10 -0.5 3,867,280 - SO3722 0.11 +0.4 3,867,416 - SO3722 0.27 -1.3 3,867,554 + SO3722 0.44 +0.5 3,867,554 + SO3722 0.44 +0.2 3,867,554 + SO3722 0.44 +1.9 3,867,554 + SO3722 0.44 +0.8 3,867,562 - SO3722 0.45 +0.4 3,867,562 - SO3722 0.45 +0.4 3,867,562 - SO3722 0.45 -1.7 3,867,569 + SO3722 0.45 -0.8 3,867,569 + SO3722 0.45 -0.2 3,867,569 + SO3722 0.45 +0.5 3,867,569 + SO3722 0.45 -0.5 3,867,577 - SO3722 0.46 -0.2 3,867,577 - SO3722 0.46 -0.0 3,867,577 - SO3722 0.46 -0.6 3,867,577 - SO3722 0.46 -1.6 3,867,577 - SO3722 0.46 -2.2 3,867,594 - SO3722 0.48 +4.1 3,867,622 + SO3722 0.52 +0.1 3,867,622 + SO3722 0.52 -2.2 3,867,725 + SO3722 0.64 +0.1 3,867,731 + SO3722 0.65 -2.2 3,867,739 - SO3722 0.66 -1.8 3,867,739 - SO3722 0.66 +0.3 3,867,739 - SO3722 0.66 +1.2 3,867,759 - SO3722 0.68 -0.0 3,867,764 - SO3722 0.69 +1.6 3,867,986 - -2.0 3,868,034 + -2.9 3,868,044 + -1.4 3,868,058 + -2.2 3,868,173 - cysC SO3723 0.20 -3.3 3,868,265 - cysC SO3723 0.35 -1.8 3,868,346 - cysC SO3723 0.48 -2.8 3,868,346 - cysC SO3723 0.48 -2.4 3,868,354 + cysC SO3723 0.50 -3.5 3,868,451 - cysC SO3723 0.65 -2.0 3,868,639 + -2.8 3,868,653 + -2.5 3,868,681 - +1.2 3,868,681 - -0.9 3,868,817 + -2.2 3,868,860 - -0.6 3,868,881 - SO3725 0.13 +1.7 3,869,002 - SO3725 0.51 +0.3 3,869,004 + SO3725 0.51 -3.4
Or see this region's nucleotide sequence