Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3592 and SO3593 are separated by 35 nucleotides SO3593 and SO3594 overlap by 1 nucleotides SO3594 and SO3595 overlap by 46 nucleotides
SO3592: SO3592 - conserved hypothetical protein (NCBI ptt file), at 3,755,361 to 3,756,275
SO3592
SO3593: SO3593 - hypothetical protein (NCBI ptt file), at 3,756,311 to 3,756,694
SO3593
SO3594: SO3594 - transcriptional regulatory protein RstA, putative (NCBI ptt file), at 3,756,694 to 3,757,407
SO3594
SO3595: SO3595 - sensor protein RstB, putative (NCBI ptt file), at 3,757,362 to 3,758,684
SO3595
Position (kb)
3756
3757 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3755.332 kb on + strand at 3755.332 kb on + strand at 3755.379 kb on + strand at 3755.390 kb on + strand at 3755.390 kb on + strand at 3755.390 kb on + strand at 3755.398 kb on - strand at 3755.398 kb on - strand at 3755.409 kb on + strand at 3755.432 kb on - strand at 3755.451 kb on - strand at 3755.532 kb on + strand, within SO3592 at 3755.546 kb on - strand, within SO3592 at 3755.649 kb on + strand, within SO3592 at 3755.649 kb on + strand, within SO3592 at 3755.649 kb on + strand, within SO3592 at 3755.657 kb on - strand, within SO3592 at 3755.657 kb on - strand, within SO3592 at 3755.657 kb on - strand, within SO3592 at 3755.657 kb on - strand, within SO3592 at 3755.659 kb on - strand, within SO3592 at 3755.706 kb on - strand, within SO3592 at 3755.716 kb on - strand, within SO3592 at 3755.763 kb on + strand, within SO3592 at 3755.773 kb on + strand, within SO3592 at 3755.773 kb on + strand, within SO3592 at 3755.773 kb on + strand, within SO3592 at 3755.773 kb on + strand, within SO3592 at 3755.773 kb on + strand, within SO3592 at 3755.781 kb on - strand, within SO3592 at 3755.781 kb on - strand, within SO3592 at 3755.781 kb on - strand, within SO3592 at 3755.781 kb on - strand, within SO3592 at 3755.781 kb on - strand, within SO3592 at 3755.781 kb on - strand, within SO3592 at 3755.781 kb on - strand, within SO3592 at 3755.797 kb on - strand, within SO3592 at 3755.813 kb on + strand, within SO3592 at 3755.823 kb on + strand, within SO3592 at 3755.833 kb on + strand, within SO3592 at 3755.841 kb on - strand, within SO3592 at 3755.841 kb on - strand, within SO3592 at 3755.841 kb on - strand, within SO3592 at 3755.905 kb on + strand, within SO3592 at 3755.908 kb on - strand, within SO3592 at 3755.910 kb on + strand, within SO3592 at 3755.917 kb on + strand, within SO3592 at 3755.918 kb on - strand, within SO3592 at 3755.918 kb on - strand, within SO3592 at 3755.927 kb on + strand, within SO3592 at 3755.934 kb on - strand, within SO3592 at 3755.987 kb on - strand, within SO3592 at 3755.994 kb on + strand, within SO3592 at 3756.110 kb on - strand, within SO3592 at 3756.143 kb on + strand, within SO3592 at 3756.261 kb on + strand at 3756.261 kb on + strand at 3756.269 kb on + strand at 3756.269 kb on - strand at 3756.280 kb on + strand at 3756.285 kb on + strand at 3756.285 kb on + strand at 3756.305 kb on + strand at 3756.323 kb on + strand at 3756.324 kb on + strand at 3756.324 kb on - strand at 3756.330 kb on + strand at 3756.331 kb on - strand at 3756.332 kb on - strand at 3756.402 kb on + strand, within SO3593 at 3756.408 kb on - strand, within SO3593 at 3756.476 kb on + strand, within SO3593 at 3756.507 kb on - strand, within SO3593 at 3756.552 kb on + strand, within SO3593 at 3756.589 kb on - strand, within SO3593 at 3756.658 kb on + strand at 3756.658 kb on + strand at 3756.666 kb on - strand at 3756.670 kb on + strand at 3756.676 kb on + strand at 3756.721 kb on + strand at 3756.820 kb on + strand, within SO3594 at 3756.820 kb on + strand, within SO3594 at 3756.820 kb on + strand, within SO3594 at 3756.820 kb on + strand, within SO3594 at 3756.820 kb on + strand, within SO3594 at 3756.828 kb on - strand, within SO3594 at 3756.828 kb on - strand, within SO3594 at 3756.828 kb on - strand, within SO3594 at 3756.828 kb on - strand, within SO3594 at 3756.828 kb on - strand, within SO3594 at 3756.848 kb on - strand, within SO3594 at 3756.881 kb on + strand, within SO3594 at 3756.881 kb on + strand, within SO3594 at 3756.890 kb on + strand, within SO3594 at 3756.890 kb on + strand, within SO3594 at 3756.890 kb on + strand, within SO3594 at 3756.890 kb on + strand, within SO3594 at 3756.898 kb on - strand, within SO3594 at 3756.898 kb on - strand, within SO3594 at 3756.898 kb on - strand, within SO3594 at 3756.898 kb on - strand, within SO3594 at 3756.898 kb on - strand, within SO3594 at 3756.937 kb on - strand, within SO3594 at 3756.937 kb on - strand, within SO3594 at 3756.937 kb on - strand, within SO3594 at 3756.945 kb on - strand, within SO3594 at 3756.947 kb on - strand, within SO3594 at 3757.008 kb on + strand, within SO3594 at 3757.022 kb on + strand, within SO3594 at 3757.099 kb on - strand, within SO3594 at 3757.113 kb on + strand, within SO3594 at 3757.113 kb on + strand, within SO3594 at 3757.113 kb on + strand, within SO3594 at 3757.113 kb on + strand, within SO3594 at 3757.121 kb on - strand, within SO3594 at 3757.216 kb on + strand, within SO3594 at 3757.222 kb on + strand, within SO3594 at 3757.301 kb on + strand, within SO3594 at 3757.301 kb on + strand, within SO3594 at 3757.411 kb on - strand at 3757.480 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,755,332 + -0.3 3,755,332 + -2.3 3,755,379 + -0.6 3,755,390 + -2.3 3,755,390 + +1.9 3,755,390 + +1.2 3,755,398 - +0.6 3,755,398 - -0.8 3,755,409 + +2.5 3,755,432 - +0.4 3,755,451 - -1.8 3,755,532 + SO3592 0.19 -2.9 3,755,546 - SO3592 0.20 +0.3 3,755,649 + SO3592 0.31 -3.1 3,755,649 + SO3592 0.31 +2.6 3,755,649 + SO3592 0.31 +0.1 3,755,657 - SO3592 0.32 -0.8 3,755,657 - SO3592 0.32 +0.7 3,755,657 - SO3592 0.32 +1.7 3,755,657 - SO3592 0.32 +0.4 3,755,659 - SO3592 0.33 +0.4 3,755,706 - SO3592 0.38 -3.1 3,755,716 - SO3592 0.39 +0.4 3,755,763 + SO3592 0.44 +0.5 3,755,773 + SO3592 0.45 -2.6 3,755,773 + SO3592 0.45 +0.3 3,755,773 + SO3592 0.45 +0.0 3,755,773 + SO3592 0.45 +1.4 3,755,773 + SO3592 0.45 +0.2 3,755,781 - SO3592 0.46 -1.5 3,755,781 - SO3592 0.46 -0.9 3,755,781 - SO3592 0.46 -1.6 3,755,781 - SO3592 0.46 -1.1 3,755,781 - SO3592 0.46 +1.7 3,755,781 - SO3592 0.46 -0.3 3,755,781 - SO3592 0.46 -3.3 3,755,797 - SO3592 0.48 -0.9 3,755,813 + SO3592 0.49 -1.3 3,755,823 + SO3592 0.50 +0.0 3,755,833 + SO3592 0.52 -2.4 3,755,841 - SO3592 0.52 +0.8 3,755,841 - SO3592 0.52 -0.5 3,755,841 - SO3592 0.52 +0.5 3,755,905 + SO3592 0.59 -2.5 3,755,908 - SO3592 0.60 +1.2 3,755,910 + SO3592 0.60 +1.0 3,755,917 + SO3592 0.61 -4.4 3,755,918 - SO3592 0.61 -0.9 3,755,918 - SO3592 0.61 -1.8 3,755,927 + SO3592 0.62 -0.6 3,755,934 - SO3592 0.63 +1.4 3,755,987 - SO3592 0.68 -2.1 3,755,994 + SO3592 0.69 +0.7 3,756,110 - SO3592 0.82 -2.9 3,756,143 + SO3592 0.85 -2.5 3,756,261 + +1.3 3,756,261 + +0.2 3,756,269 + +0.3 3,756,269 - +0.9 3,756,280 + -0.5 3,756,285 + +1.3 3,756,285 + -0.6 3,756,305 + -2.6 3,756,323 + -0.7 3,756,324 + +0.5 3,756,324 - -0.5 3,756,330 + -2.8 3,756,331 - +0.3 3,756,332 - +1.8 3,756,402 + SO3593 0.24 -0.3 3,756,408 - SO3593 0.25 -3.5 3,756,476 + SO3593 0.43 -0.2 3,756,507 - SO3593 0.51 -1.3 3,756,552 + SO3593 0.63 +1.0 3,756,589 - SO3593 0.72 -1.0 3,756,658 + -0.4 3,756,658 + -1.3 3,756,666 - -2.1 3,756,670 + +1.9 3,756,676 + -0.9 3,756,721 + -2.2 3,756,820 + SO3594 0.18 +0.9 3,756,820 + SO3594 0.18 -0.3 3,756,820 + SO3594 0.18 -0.7 3,756,820 + SO3594 0.18 -0.7 3,756,820 + SO3594 0.18 +0.3 3,756,828 - SO3594 0.19 +0.5 3,756,828 - SO3594 0.19 +0.1 3,756,828 - SO3594 0.19 +0.7 3,756,828 - SO3594 0.19 -1.5 3,756,828 - SO3594 0.19 -1.0 3,756,848 - SO3594 0.22 +0.6 3,756,881 + SO3594 0.26 -1.3 3,756,881 + SO3594 0.26 +1.3 3,756,890 + SO3594 0.27 +0.0 3,756,890 + SO3594 0.27 -0.3 3,756,890 + SO3594 0.27 -2.5 3,756,890 + SO3594 0.27 +0.9 3,756,898 - SO3594 0.29 +0.3 3,756,898 - SO3594 0.29 +1.8 3,756,898 - SO3594 0.29 +0.4 3,756,898 - SO3594 0.29 +1.8 3,756,898 - SO3594 0.29 +0.2 3,756,937 - SO3594 0.34 +0.8 3,756,937 - SO3594 0.34 -0.2 3,756,937 - SO3594 0.34 -1.1 3,756,945 - SO3594 0.35 +1.4 3,756,947 - SO3594 0.35 -0.3 3,757,008 + SO3594 0.44 +0.9 3,757,022 + SO3594 0.46 -1.3 3,757,099 - SO3594 0.57 +2.0 3,757,113 + SO3594 0.59 +0.2 3,757,113 + SO3594 0.59 -1.6 3,757,113 + SO3594 0.59 -0.9 3,757,113 + SO3594 0.59 -0.9 3,757,121 - SO3594 0.60 -0.8 3,757,216 + SO3594 0.73 +0.3 3,757,222 + SO3594 0.74 +0.2 3,757,301 + SO3594 0.85 +2.5 3,757,301 + SO3594 0.85 -1.6 3,757,411 - -0.0 3,757,480 - -2.1
Or see this region's nucleotide sequence