Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sp16SH and SO3575 are separated by 777 nucleotides SO3575 and SO3576 are separated by 90 nucleotides SO3576 and clpB are separated by 421 nucleotides
SO_r015: Sp16SH - 16S ribosomal RNA (RefSeq), at 3,738,378 to 3,739,906
Sp16SH
SO3575: SO3575 - CDP-diacylglycerol--serine O-phosphatidyltransferase, putative (NCBI ptt file), at 3,740,684 to 3,741,493
SO3575
SO3576: SO3576 - conserved hypothetical protein (NCBI ptt file), at 3,741,584 to 3,742,048
SO3576
SO3577: clpB - clpB protein (NCBI ptt file), at 3,742,470 to 3,745,043
clpB
Position (kb)
3740
3741
3742 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3740.402 kb on + strand at 3740.471 kb on + strand at 3740.528 kb on + strand at 3740.558 kb on + strand at 3740.585 kb on + strand at 3740.585 kb on + strand at 3740.593 kb on - strand at 3740.607 kb on - strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.637 kb on + strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.645 kb on - strand at 3740.652 kb on - strand at 3740.737 kb on - strand at 3740.797 kb on + strand, within SO3575 at 3740.821 kb on + strand, within SO3575 at 3740.864 kb on - strand, within SO3575 at 3740.990 kb on - strand, within SO3575 at 3741.070 kb on - strand, within SO3575 at 3741.123 kb on + strand, within SO3575 at 3741.140 kb on + strand, within SO3575 at 3741.148 kb on - strand, within SO3575 at 3741.148 kb on - strand, within SO3575 at 3741.148 kb on - strand, within SO3575 at 3741.148 kb on - strand, within SO3575 at 3741.164 kb on + strand, within SO3575 at 3741.214 kb on - strand, within SO3575 at 3741.264 kb on - strand, within SO3575 at 3741.374 kb on - strand, within SO3575 at 3741.453 kb on - strand at 3741.464 kb on - strand at 3741.469 kb on - strand at 3741.495 kb on + strand at 3741.495 kb on + strand at 3741.503 kb on - strand at 3741.503 kb on - strand at 3741.505 kb on - strand at 3741.527 kb on + strand at 3741.537 kb on - strand at 3741.607 kb on - strand at 3741.611 kb on + strand at 3741.628 kb on - strand at 3741.630 kb on + strand at 3741.638 kb on - strand, within SO3576 at 3741.699 kb on + strand, within SO3576 at 3741.748 kb on + strand, within SO3576 at 3741.748 kb on - strand, within SO3576 at 3741.845 kb on + strand, within SO3576 at 3741.845 kb on + strand, within SO3576 at 3741.874 kb on + strand, within SO3576 at 3741.877 kb on - strand, within SO3576 at 3741.906 kb on + strand, within SO3576 at 3741.906 kb on + strand, within SO3576 at 3741.924 kb on - strand, within SO3576 at 3741.927 kb on + strand, within SO3576 at 3741.927 kb on + strand, within SO3576 at 3741.936 kb on - strand, within SO3576 at 3741.947 kb on + strand, within SO3576 at 3742.063 kb on - strand at 3742.063 kb on - strand at 3742.063 kb on - strand at 3742.065 kb on + strand at 3742.073 kb on - strand at 3742.209 kb on + strand at 3742.335 kb on + strand at 3742.335 kb on + strand at 3742.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,740,402 + +0.1 3,740,471 + -2.1 3,740,528 + +0.3 3,740,558 + -3.2 3,740,585 + -3.0 3,740,585 + +1.1 3,740,593 - -2.1 3,740,607 - -0.8 3,740,637 + +0.1 3,740,637 + +1.7 3,740,637 + -3.7 3,740,637 + +2.6 3,740,637 + +1.3 3,740,637 + -0.9 3,740,637 + +0.3 3,740,637 + -1.5 3,740,637 + +1.4 3,740,637 + -2.4 3,740,637 + -1.2 3,740,637 + +1.0 3,740,637 + +2.0 3,740,637 + -2.0 3,740,637 + -0.2 3,740,637 + +1.8 3,740,645 - -1.3 3,740,645 - -1.6 3,740,645 - -0.3 3,740,645 - +1.0 3,740,645 - -1.6 3,740,645 - -0.7 3,740,645 - +0.3 3,740,645 - +1.4 3,740,645 - -0.5 3,740,645 - +1.1 3,740,645 - -0.6 3,740,645 - +0.3 3,740,645 - +0.1 3,740,645 - -3.0 3,740,645 - -1.6 3,740,645 - +0.9 3,740,652 - +1.8 3,740,737 - +2.4 3,740,797 + SO3575 0.14 -0.2 3,740,821 + SO3575 0.17 +2.8 3,740,864 - SO3575 0.22 +3.0 3,740,990 - SO3575 0.38 +3.2 3,741,070 - SO3575 0.48 +2.3 3,741,123 + SO3575 0.54 +0.9 3,741,140 + SO3575 0.56 +0.2 3,741,148 - SO3575 0.57 +0.3 3,741,148 - SO3575 0.57 +2.1 3,741,148 - SO3575 0.57 +1.5 3,741,148 - SO3575 0.57 +1.8 3,741,164 + SO3575 0.59 +2.1 3,741,214 - SO3575 0.65 +2.4 3,741,264 - SO3575 0.72 -0.5 3,741,374 - SO3575 0.85 +1.3 3,741,453 - +1.9 3,741,464 - +2.0 3,741,469 - +1.2 3,741,495 + +2.0 3,741,495 + -0.6 3,741,503 - +2.5 3,741,503 - -0.5 3,741,505 - +1.5 3,741,527 + +2.0 3,741,537 - +1.7 3,741,607 - +1.8 3,741,611 + -2.1 3,741,628 - -2.5 3,741,630 + +0.1 3,741,638 - SO3576 0.12 +0.2 3,741,699 + SO3576 0.25 -4.5 3,741,748 + SO3576 0.35 +0.3 3,741,748 - SO3576 0.35 -1.4 3,741,845 + SO3576 0.56 +1.3 3,741,845 + SO3576 0.56 -0.3 3,741,874 + SO3576 0.62 +0.9 3,741,877 - SO3576 0.63 +1.1 3,741,906 + SO3576 0.69 -2.0 3,741,906 + SO3576 0.69 -1.1 3,741,924 - SO3576 0.73 +1.0 3,741,927 + SO3576 0.74 +0.5 3,741,927 + SO3576 0.74 -1.0 3,741,936 - SO3576 0.76 +0.2 3,741,947 + SO3576 0.78 -3.2 3,742,063 - -0.9 3,742,063 - -2.8 3,742,063 - +0.1 3,742,065 + -2.5 3,742,073 - +0.1 3,742,209 + +0.7 3,742,335 + +1.0 3,742,335 + -2.4 3,742,345 - -1.0
Or see this region's nucleotide sequence