Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpsT and hlyU are separated by 60 nucleotides hlyU and SO3539 are separated by 296 nucleotides SO3539 and SO3540 are separated by 75 nucleotides SO3540 and SO3541 are separated by 106 nucleotides
SO3537: rpsT - ribosomal protein S20 (NCBI ptt file), at 3,691,476 to 3,691,742
rpsT
SO3538: hlyU - transcriptional regulator HlyU (NCBI ptt file), at 3,691,803 to 3,692,099
hlyU
SO3539: SO3539 - peptidase, M28D family (NCBI ptt file), at 3,692,396 to 3,693,640
SO3539
SO3540: SO3540 - conserved hypothetical protein (NCBI ptt file), at 3,693,716 to 3,694,489
SO3540
SO3541: SO3541 - sodium:alanine symporter family protein (NCBI ptt file), at 3,694,596 to 3,696,062
SO3541
Position (kb)
3692
3693
3694 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3691.863 kb on - strand, within hlyU at 3691.866 kb on + strand, within hlyU at 3691.872 kb on - strand, within hlyU at 3691.874 kb on - strand, within hlyU at 3691.923 kb on + strand, within hlyU at 3692.015 kb on + strand, within hlyU at 3692.045 kb on + strand, within hlyU at 3692.069 kb on - strand, within hlyU at 3692.069 kb on - strand, within hlyU at 3692.069 kb on - strand, within hlyU at 3692.071 kb on + strand at 3692.078 kb on - strand at 3692.079 kb on - strand at 3692.119 kb on + strand at 3692.141 kb on + strand at 3692.143 kb on - strand at 3692.143 kb on - strand at 3692.146 kb on + strand at 3692.154 kb on + strand at 3692.154 kb on + strand at 3692.161 kb on - strand at 3692.278 kb on - strand at 3692.359 kb on - strand at 3692.369 kb on + strand at 3692.378 kb on - strand at 3692.403 kb on - strand at 3692.454 kb on - strand at 3692.472 kb on + strand at 3692.472 kb on + strand at 3692.488 kb on + strand at 3692.499 kb on + strand at 3692.553 kb on + strand, within SO3539 at 3692.587 kb on + strand, within SO3539 at 3692.603 kb on - strand, within SO3539 at 3692.603 kb on - strand, within SO3539 at 3692.623 kb on + strand, within SO3539 at 3692.763 kb on + strand, within SO3539 at 3692.764 kb on + strand, within SO3539 at 3692.786 kb on + strand, within SO3539 at 3692.794 kb on - strand, within SO3539 at 3692.834 kb on - strand, within SO3539 at 3692.855 kb on + strand, within SO3539 at 3692.905 kb on + strand, within SO3539 at 3692.915 kb on + strand, within SO3539 at 3693.036 kb on + strand, within SO3539 at 3693.036 kb on + strand, within SO3539 at 3693.183 kb on + strand, within SO3539 at 3693.183 kb on + strand, within SO3539 at 3693.189 kb on + strand, within SO3539 at 3693.191 kb on - strand, within SO3539 at 3693.191 kb on - strand, within SO3539 at 3693.191 kb on - strand, within SO3539 at 3693.191 kb on - strand, within SO3539 at 3693.191 kb on - strand, within SO3539 at 3693.191 kb on - strand, within SO3539 at 3693.197 kb on - strand, within SO3539 at 3693.236 kb on + strand, within SO3539 at 3693.243 kb on - strand, within SO3539 at 3693.243 kb on - strand, within SO3539 at 3693.243 kb on - strand, within SO3539 at 3693.366 kb on + strand, within SO3539 at 3693.366 kb on + strand, within SO3539 at 3693.415 kb on - strand, within SO3539 at 3693.415 kb on - strand, within SO3539 at 3693.439 kb on - strand, within SO3539 at 3693.447 kb on + strand, within SO3539 at 3693.447 kb on + strand, within SO3539 at 3693.455 kb on - strand, within SO3539 at 3693.455 kb on - strand, within SO3539 at 3693.473 kb on + strand, within SO3539 at 3693.481 kb on - strand, within SO3539 at 3693.510 kb on + strand, within SO3539 at 3693.606 kb on - strand at 3693.617 kb on + strand at 3693.625 kb on - strand at 3693.625 kb on - strand at 3693.662 kb on - strand at 3693.665 kb on + strand at 3693.665 kb on + strand at 3693.665 kb on + strand at 3693.678 kb on + strand at 3693.687 kb on + strand at 3693.690 kb on + strand at 3693.726 kb on - strand at 3693.772 kb on + strand at 3693.787 kb on + strand at 3693.795 kb on - strand, within SO3540 at 3693.882 kb on + strand, within SO3540 at 3693.882 kb on + strand, within SO3540 at 3693.882 kb on + strand, within SO3540 at 3693.882 kb on + strand, within SO3540 at 3693.890 kb on - strand, within SO3540 at 3693.890 kb on - strand, within SO3540 at 3693.890 kb on - strand, within SO3540 at 3693.890 kb on - strand, within SO3540 at 3693.911 kb on + strand, within SO3540 at 3693.982 kb on + strand, within SO3540 at 3693.990 kb on - strand, within SO3540 at 3693.996 kb on + strand, within SO3540 at 3693.996 kb on + strand, within SO3540 at 3694.001 kb on + strand, within SO3540 at 3694.027 kb on - strand, within SO3540 at 3694.099 kb on + strand, within SO3540 at 3694.150 kb on - strand, within SO3540 at 3694.192 kb on - strand, within SO3540 at 3694.200 kb on - strand, within SO3540 at 3694.206 kb on - strand, within SO3540 at 3694.248 kb on + strand, within SO3540 at 3694.371 kb on - strand, within SO3540 at 3694.390 kb on + strand, within SO3540 at 3694.398 kb on - strand, within SO3540 at 3694.487 kb on + strand at 3694.574 kb on + strand at 3694.582 kb on - strand at 3694.582 kb on - strand at 3694.582 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,691,863 - hlyU SO3538 0.20 -0.0 3,691,866 + hlyU SO3538 0.21 -0.7 3,691,872 - hlyU SO3538 0.23 -2.5 3,691,874 - hlyU SO3538 0.24 -2.7 3,691,923 + hlyU SO3538 0.40 -1.3 3,692,015 + hlyU SO3538 0.71 +2.1 3,692,045 + hlyU SO3538 0.81 -1.3 3,692,069 - hlyU SO3538 0.90 -1.2 3,692,069 - hlyU SO3538 0.90 +0.9 3,692,069 - hlyU SO3538 0.90 -0.0 3,692,071 + -0.4 3,692,078 - -0.9 3,692,079 - -2.4 3,692,119 + +0.4 3,692,141 + -0.8 3,692,143 - +2.0 3,692,143 - -0.3 3,692,146 + +1.9 3,692,154 + -1.9 3,692,154 + +1.1 3,692,161 - +0.5 3,692,278 - +0.4 3,692,359 - +1.8 3,692,369 + -0.4 3,692,378 - +0.0 3,692,403 - +1.6 3,692,454 - +0.5 3,692,472 + -0.9 3,692,472 + +1.1 3,692,488 + -1.5 3,692,499 + -0.5 3,692,553 + SO3539 0.13 -0.2 3,692,587 + SO3539 0.15 +0.4 3,692,603 - SO3539 0.17 -3.1 3,692,603 - SO3539 0.17 +1.3 3,692,623 + SO3539 0.18 +0.6 3,692,763 + SO3539 0.29 +0.4 3,692,764 + SO3539 0.30 -0.2 3,692,786 + SO3539 0.31 -0.0 3,692,794 - SO3539 0.32 +0.2 3,692,834 - SO3539 0.35 +2.1 3,692,855 + SO3539 0.37 +1.5 3,692,905 + SO3539 0.41 +0.3 3,692,915 + SO3539 0.42 +2.6 3,693,036 + SO3539 0.51 +0.3 3,693,036 + SO3539 0.51 +0.6 3,693,183 + SO3539 0.63 -0.7 3,693,183 + SO3539 0.63 +1.2 3,693,189 + SO3539 0.64 +0.1 3,693,191 - SO3539 0.64 +0.6 3,693,191 - SO3539 0.64 +0.1 3,693,191 - SO3539 0.64 +1.1 3,693,191 - SO3539 0.64 -1.9 3,693,191 - SO3539 0.64 +0.6 3,693,191 - SO3539 0.64 -0.1 3,693,197 - SO3539 0.64 -1.0 3,693,236 + SO3539 0.67 +0.2 3,693,243 - SO3539 0.68 -0.0 3,693,243 - SO3539 0.68 -3.4 3,693,243 - SO3539 0.68 +0.8 3,693,366 + SO3539 0.78 -1.5 3,693,366 + SO3539 0.78 +1.4 3,693,415 - SO3539 0.82 +1.6 3,693,415 - SO3539 0.82 +1.2 3,693,439 - SO3539 0.84 -3.6 3,693,447 + SO3539 0.84 -0.9 3,693,447 + SO3539 0.84 +0.4 3,693,455 - SO3539 0.85 +0.6 3,693,455 - SO3539 0.85 +0.9 3,693,473 + SO3539 0.87 +1.3 3,693,481 - SO3539 0.87 -2.5 3,693,510 + SO3539 0.89 +1.6 3,693,606 - +0.8 3,693,617 + +1.2 3,693,625 - +2.4 3,693,625 - +0.6 3,693,662 - +1.1 3,693,665 + +1.2 3,693,665 + +0.7 3,693,665 + +1.0 3,693,678 + +0.2 3,693,687 + +1.1 3,693,690 + +1.0 3,693,726 - -0.5 3,693,772 + -0.6 3,693,787 + -3.3 3,693,795 - SO3540 0.10 +1.9 3,693,882 + SO3540 0.21 +0.1 3,693,882 + SO3540 0.21 +0.4 3,693,882 + SO3540 0.21 +0.6 3,693,882 + SO3540 0.21 +1.5 3,693,890 - SO3540 0.22 +0.6 3,693,890 - SO3540 0.22 -1.8 3,693,890 - SO3540 0.22 +1.1 3,693,890 - SO3540 0.22 -0.5 3,693,911 + SO3540 0.25 +1.8 3,693,982 + SO3540 0.34 +0.0 3,693,990 - SO3540 0.35 +2.7 3,693,996 + SO3540 0.36 +2.2 3,693,996 + SO3540 0.36 -1.1 3,694,001 + SO3540 0.37 +0.8 3,694,027 - SO3540 0.40 -0.5 3,694,099 + SO3540 0.49 +1.9 3,694,150 - SO3540 0.56 -0.0 3,694,192 - SO3540 0.61 -1.0 3,694,200 - SO3540 0.63 -0.6 3,694,206 - SO3540 0.63 -0.6 3,694,248 + SO3540 0.69 -3.0 3,694,371 - SO3540 0.85 +0.7 3,694,390 + SO3540 0.87 +0.7 3,694,398 - SO3540 0.88 -0.3 3,694,487 + +1.1 3,694,574 + -2.3 3,694,582 - +0.9 3,694,582 - -2.3 3,694,582 - +1.2
Or see this region's nucleotide sequence