Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3421 and yfiA-2 are separated by 300 nucleotides
SO3421: SO3421 - b-type cytochrome, putative (NCBI ptt file), at 3,560,585 to 3,561,277
SO3421
SO3422: yfiA-2 - ribosomal subunit interface protein (NCBI ptt file), at 3,561,578 to 3,561,934
yfiA-2
Position (kb)
3561
3562 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3560.585 kb on + strand at 3560.585 kb on + strand at 3560.593 kb on - strand at 3560.593 kb on - strand at 3560.607 kb on - strand at 3560.608 kb on + strand at 3560.624 kb on + strand at 3560.632 kb on - strand at 3560.741 kb on - strand, within SO3421 at 3560.812 kb on - strand, within SO3421 at 3560.890 kb on - strand, within SO3421 at 3560.891 kb on + strand, within SO3421 at 3560.964 kb on + strand, within SO3421 at 3560.986 kb on + strand, within SO3421 at 3560.993 kb on + strand, within SO3421 at 3560.994 kb on - strand, within SO3421 at 3561.012 kb on - strand, within SO3421 at 3561.038 kb on + strand, within SO3421 at 3561.056 kb on - strand, within SO3421 at 3561.056 kb on - strand, within SO3421 at 3561.102 kb on + strand, within SO3421 at 3561.102 kb on + strand, within SO3421 at 3561.136 kb on - strand, within SO3421 at 3561.193 kb on - strand, within SO3421 at 3561.281 kb on + strand at 3561.282 kb on - strand at 3561.282 kb on - strand at 3561.282 kb on - strand at 3561.286 kb on + strand at 3561.289 kb on - strand at 3561.294 kb on - strand at 3561.294 kb on - strand at 3561.314 kb on + strand at 3561.369 kb on + strand at 3561.405 kb on - strand at 3561.415 kb on - strand at 3561.483 kb on + strand at 3561.483 kb on + strand at 3561.491 kb on - strand at 3561.569 kb on + strand at 3561.569 kb on + strand at 3561.594 kb on - strand at 3561.614 kb on + strand, within yfiA-2 at 3561.629 kb on - strand, within yfiA-2 at 3561.676 kb on + strand, within yfiA-2 at 3561.684 kb on - strand, within yfiA-2 at 3561.704 kb on - strand, within yfiA-2 at 3561.766 kb on + strand, within yfiA-2 at 3561.766 kb on + strand, within yfiA-2 at 3561.781 kb on + strand, within yfiA-2 at 3561.783 kb on - strand, within yfiA-2 at 3561.807 kb on - strand, within yfiA-2 at 3561.826 kb on + strand, within yfiA-2 at 3561.833 kb on + strand, within yfiA-2 at 3561.840 kb on + strand, within yfiA-2 at 3561.841 kb on - strand, within yfiA-2 at 3561.841 kb on - strand, within yfiA-2 at 3561.880 kb on + strand, within yfiA-2 at 3561.880 kb on + strand, within yfiA-2 at 3561.905 kb on - strand at 3561.905 kb on - strand at 3561.907 kb on + strand at 3561.907 kb on + strand at 3561.907 kb on + strand at 3561.914 kb on - strand at 3561.915 kb on - strand at 3561.916 kb on - strand at 3561.917 kb on + strand at 3561.951 kb on - strand at 3561.951 kb on - strand at 3561.963 kb on + strand at 3561.963 kb on + strand at 3561.963 kb on + strand at 3561.973 kb on + strand at 3561.973 kb on + strand at 3561.981 kb on - strand at 3561.981 kb on - strand at 3561.998 kb on + strand at 3562.045 kb on - strand at 3562.076 kb on + strand at 3562.076 kb on + strand at 3562.097 kb on + strand at 3562.105 kb on - strand at 3562.105 kb on - strand at 3562.110 kb on - strand at 3562.140 kb on + strand at 3562.143 kb on + strand at 3562.153 kb on + strand at 3562.153 kb on + strand at 3562.161 kb on - strand at 3562.163 kb on + strand at 3562.241 kb on - strand at 3562.253 kb on + strand at 3562.253 kb on + strand at 3562.261 kb on - strand at 3562.265 kb on + strand at 3562.273 kb on - strand at 3562.319 kb on - strand at 3562.319 kb on - strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.333 kb on + strand at 3562.336 kb on + strand at 3562.336 kb on + strand at 3562.336 kb on - strand at 3562.340 kb on + strand at 3562.340 kb on + strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.341 kb on - strand at 3562.343 kb on + strand at 3562.343 kb on + strand at 3562.344 kb on - strand at 3562.379 kb on + strand at 3562.379 kb on + strand at 3562.379 kb on + strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.387 kb on - strand at 3562.388 kb on + strand at 3562.389 kb on + strand at 3562.395 kb on - strand at 3562.395 kb on - strand at 3562.414 kb on - strand at 3562.424 kb on - strand at 3562.433 kb on - strand at 3562.433 kb on - strand at 3562.433 kb on - strand at 3562.442 kb on - strand at 3562.447 kb on - strand at 3562.450 kb on + strand at 3562.453 kb on - strand at 3562.528 kb on - strand at 3562.538 kb on - strand at 3562.556 kb on - strand at 3562.578 kb on - strand at 3562.578 kb on - strand at 3562.578 kb on - strand at 3562.604 kb on + strand at 3562.612 kb on - strand at 3562.612 kb on - strand at 3562.616 kb on + strand at 3562.616 kb on + strand at 3562.616 kb on + strand at 3562.624 kb on - strand at 3562.626 kb on + strand at 3562.626 kb on - strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.639 kb on + strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.647 kb on - strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.649 kb on + strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.657 kb on - strand at 3562.707 kb on - strand at 3562.709 kb on - strand at 3562.736 kb on + strand at 3562.736 kb on + strand at 3562.736 kb on + strand at 3562.736 kb on + strand at 3562.736 kb on + strand at 3562.736 kb on + strand at 3562.738 kb on + strand at 3562.738 kb on + strand at 3562.744 kb on - strand at 3562.744 kb on - strand at 3562.744 kb on - strand at 3562.744 kb on - strand at 3562.744 kb on - strand at 3562.746 kb on - strand at 3562.765 kb on + strand at 3562.774 kb on + strand at 3562.782 kb on + strand at 3562.806 kb on + strand at 3562.810 kb on + strand at 3562.810 kb on + strand at 3562.818 kb on - strand at 3562.818 kb on - strand at 3562.818 kb on - strand at 3562.818 kb on - strand at 3562.835 kb on + strand at 3562.837 kb on + strand at 3562.841 kb on + strand at 3562.842 kb on + strand at 3562.843 kb on + strand at 3562.850 kb on - strand at 3562.850 kb on - strand at 3562.850 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,560,585 + +1.2 3,560,585 + +1.0 3,560,593 - -0.6 3,560,593 - +0.8 3,560,607 - +0.4 3,560,608 + +0.6 3,560,624 + +1.7 3,560,632 - +1.1 3,560,741 - SO3421 0.23 +0.4 3,560,812 - SO3421 0.33 +1.8 3,560,890 - SO3421 0.44 +0.1 3,560,891 + SO3421 0.44 -1.2 3,560,964 + SO3421 0.55 +1.4 3,560,986 + SO3421 0.58 +2.2 3,560,993 + SO3421 0.59 +1.6 3,560,994 - SO3421 0.59 -0.5 3,561,012 - SO3421 0.62 -2.6 3,561,038 + SO3421 0.65 +1.6 3,561,056 - SO3421 0.68 -0.4 3,561,056 - SO3421 0.68 -1.7 3,561,102 + SO3421 0.75 +0.6 3,561,102 + SO3421 0.75 +0.2 3,561,136 - SO3421 0.80 -1.2 3,561,193 - SO3421 0.88 -0.1 3,561,281 + +0.4 3,561,282 - +0.2 3,561,282 - -1.0 3,561,282 - -0.1 3,561,286 + -0.4 3,561,289 - -2.0 3,561,294 - -1.6 3,561,294 - +0.3 3,561,314 + +1.8 3,561,369 + -0.4 3,561,405 - -0.1 3,561,415 - -0.5 3,561,483 + -0.6 3,561,483 + -0.1 3,561,491 - +0.1 3,561,569 + -1.5 3,561,569 + -0.8 3,561,594 - -1.9 3,561,614 + yfiA-2 SO3422 0.10 -2.2 3,561,629 - yfiA-2 SO3422 0.14 -0.9 3,561,676 + yfiA-2 SO3422 0.27 -2.5 3,561,684 - yfiA-2 SO3422 0.30 -0.6 3,561,704 - yfiA-2 SO3422 0.35 -1.8 3,561,766 + yfiA-2 SO3422 0.53 +1.2 3,561,766 + yfiA-2 SO3422 0.53 -3.8 3,561,781 + yfiA-2 SO3422 0.57 -2.5 3,561,783 - yfiA-2 SO3422 0.57 -0.1 3,561,807 - yfiA-2 SO3422 0.64 +0.3 3,561,826 + yfiA-2 SO3422 0.69 -2.8 3,561,833 + yfiA-2 SO3422 0.71 -3.5 3,561,840 + yfiA-2 SO3422 0.73 -1.0 3,561,841 - yfiA-2 SO3422 0.74 -2.4 3,561,841 - yfiA-2 SO3422 0.74 -0.0 3,561,880 + yfiA-2 SO3422 0.85 +0.5 3,561,880 + yfiA-2 SO3422 0.85 -0.9 3,561,905 - +1.1 3,561,905 - -1.2 3,561,907 + -4.3 3,561,907 + +0.2 3,561,907 + +3.1 3,561,914 - +1.0 3,561,915 - +0.6 3,561,916 - -0.4 3,561,917 + +0.2 3,561,951 - +1.8 3,561,951 - +0.3 3,561,963 + +0.3 3,561,963 + +0.3 3,561,963 + -0.5 3,561,973 + +0.2 3,561,973 + -0.2 3,561,981 - +0.5 3,561,981 - +1.0 3,561,998 + +1.3 3,562,045 - +1.3 3,562,076 + -0.6 3,562,076 + -0.4 3,562,097 + +2.7 3,562,105 - +0.2 3,562,105 - +3.1 3,562,110 - +1.3 3,562,140 + +1.0 3,562,143 + +0.8 3,562,153 + -0.3 3,562,153 + +0.7 3,562,161 - -3.0 3,562,163 + +0.0 3,562,241 - -2.4 3,562,253 + +1.6 3,562,253 + +0.8 3,562,261 - +1.5 3,562,265 + -0.1 3,562,273 - +1.7 3,562,319 - -0.3 3,562,319 - +1.9 3,562,333 + -2.0 3,562,333 + +0.7 3,562,333 + -0.0 3,562,333 + +1.0 3,562,333 + +1.8 3,562,333 + -0.0 3,562,333 + +0.8 3,562,333 + +0.3 3,562,333 + -2.1 3,562,333 + +0.7 3,562,336 + -0.8 3,562,336 + -0.0 3,562,336 - -0.6 3,562,340 + +2.7 3,562,340 + +2.9 3,562,341 - +0.7 3,562,341 - +1.6 3,562,341 - +2.2 3,562,341 - +0.6 3,562,341 - +2.4 3,562,341 - -1.0 3,562,341 - +0.0 3,562,343 + +0.5 3,562,343 + +0.8 3,562,344 - +1.9 3,562,379 + -0.1 3,562,379 + -1.5 3,562,379 + +0.3 3,562,387 - -0.2 3,562,387 - +0.5 3,562,387 - +2.2 3,562,387 - -0.5 3,562,387 - +0.2 3,562,387 - -0.4 3,562,387 - +4.0 3,562,388 + +0.6 3,562,389 + +0.1 3,562,395 - +1.5 3,562,395 - +0.2 3,562,414 - +0.7 3,562,424 - -0.1 3,562,433 - +2.0 3,562,433 - +0.6 3,562,433 - -3.2 3,562,442 - -2.0 3,562,447 - -0.2 3,562,450 + +2.7 3,562,453 - -0.2 3,562,528 - -0.4 3,562,538 - -0.5 3,562,556 - -0.5 3,562,578 - +0.2 3,562,578 - +0.5 3,562,578 - +0.0 3,562,604 + -0.7 3,562,612 - -2.7 3,562,612 - +1.2 3,562,616 + -0.0 3,562,616 + +2.9 3,562,616 + +0.7 3,562,624 - +2.2 3,562,626 + +1.4 3,562,626 - +1.2 3,562,639 + +1.9 3,562,639 + -0.7 3,562,639 + -3.3 3,562,639 + +1.4 3,562,639 + +0.8 3,562,639 + +0.4 3,562,639 + +0.9 3,562,639 + -1.1 3,562,639 + -0.5 3,562,639 + -1.0 3,562,639 + -2.2 3,562,639 + -3.6 3,562,647 - +1.9 3,562,647 - +0.9 3,562,647 - +2.5 3,562,647 - +0.9 3,562,647 - -0.0 3,562,647 - +0.0 3,562,647 - +1.4 3,562,647 - +1.2 3,562,647 - +1.0 3,562,647 - +0.1 3,562,647 - -1.2 3,562,647 - -0.6 3,562,649 + -2.8 3,562,649 + +1.7 3,562,649 + +0.6 3,562,649 + +0.0 3,562,649 + +0.4 3,562,649 + -1.9 3,562,649 + -0.3 3,562,649 + +0.6 3,562,649 + -0.3 3,562,649 + -0.5 3,562,649 + +0.6 3,562,649 + +0.4 3,562,649 + +0.1 3,562,649 + +1.0 3,562,649 + -0.5 3,562,649 + +1.6 3,562,649 + -1.6 3,562,649 + +0.0 3,562,649 + -3.1 3,562,649 + -2.3 3,562,649 + +0.6 3,562,649 + -1.7 3,562,649 + +0.3 3,562,649 + -1.5 3,562,649 + -1.1 3,562,649 + +0.0 3,562,649 + -0.6 3,562,649 + -0.7 3,562,649 + +0.8 3,562,657 - +1.2 3,562,657 - +0.3 3,562,657 - +1.3 3,562,657 - -1.7 3,562,657 - -0.5 3,562,657 - +0.6 3,562,657 - +1.3 3,562,657 - +2.2 3,562,657 - +2.0 3,562,657 - +1.1 3,562,657 - +0.2 3,562,657 - +3.4 3,562,657 - +1.1 3,562,657 - -1.4 3,562,657 - +0.1 3,562,707 - +1.5 3,562,709 - +1.0 3,562,736 + +0.2 3,562,736 + +0.7 3,562,736 + +0.2 3,562,736 + -3.6 3,562,736 + -2.0 3,562,736 + -1.4 3,562,738 + +0.2 3,562,738 + -2.8 3,562,744 - +0.7 3,562,744 - +1.1 3,562,744 - -0.6 3,562,744 - +2.4 3,562,744 - -0.3 3,562,746 - -0.2 3,562,765 + +2.5 3,562,774 + -1.0 3,562,782 + -0.1 3,562,806 + +0.2 3,562,810 + -0.7 3,562,810 + +2.8 3,562,818 - +0.8 3,562,818 - +0.2 3,562,818 - -0.3 3,562,818 - -0.1 3,562,835 + +0.9 3,562,837 + +0.5 3,562,841 + -2.3 3,562,842 + +2.6 3,562,843 + -1.4 3,562,850 - +1.3 3,562,850 - +0.0 3,562,850 - -0.4
Or see this region's nucleotide sequence