Strain Fitness in Shewanella oneidensis MR-1 around SO3352

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpilT and SO3352 are separated by 35 nucleotidesSO3352 and proC are separated by 224 nucleotides SO3351: pilT - twitching motility protein PilT (NCBI ptt file), at 3,495,991 to 3,497,028 pilT SO3352: SO3352 - conserved hypothetical protein TIGR00044 (NCBI ptt file), at 3,497,064 to 3,497,777 SO3352 SO3354: proC - pyrroline-5-carboxylate reductase (NCBI ptt file), at 3,498,002 to 3,498,820 proC Position (kb) 3497 3498Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 3496.071 kb on - strandat 3496.077 kb on + strandat 3496.077 kb on + strandat 3496.077 kb on + strandat 3496.077 kb on + strandat 3496.077 kb on + strandat 3496.077 kb on + strandat 3496.077 kb on + strandat 3496.085 kb on - strandat 3496.085 kb on - strandat 3496.085 kb on - strandat 3496.085 kb on - strandat 3496.085 kb on - strandat 3496.094 kb on + strandat 3496.158 kb on - strand, within pilTat 3496.375 kb on - strand, within pilTat 3496.400 kb on + strand, within pilTat 3496.441 kb on - strand, within pilTat 3496.489 kb on + strand, within pilTat 3496.566 kb on - strand, within pilTat 3496.621 kb on - strand, within pilTat 3496.757 kb on - strand, within pilTat 3496.804 kb on - strand, within pilTat 3496.806 kb on + strand, within pilTat 3496.875 kb on + strand, within pilTat 3496.878 kb on - strand, within pilTat 3496.882 kb on + strand, within pilTat 3496.890 kb on - strand, within pilTat 3496.890 kb on - strand, within pilTat 3496.904 kb on + strand, within pilTat 3496.972 kb on + strandat 3496.972 kb on + strandat 3497.099 kb on + strandat 3497.099 kb on + strandat 3497.099 kb on + strandat 3497.107 kb on - strandat 3497.107 kb on - strandat 3497.353 kb on - strand, within SO3352at 3497.545 kb on - strand, within SO3352at 3497.545 kb on - strand, within SO3352at 3497.545 kb on - strand, within SO3352at 3497.615 kb on - strand, within SO3352at 3497.793 kb on + strandat 3497.954 kb on + strandat 3497.974 kb on + strandat 3497.974 kb on + strandat 3497.974 kb on + strandat 3497.974 kb on + strandat 3497.982 kb on - strandat 3498.034 kb on + strandat 3498.063 kb on + strandat 3498.094 kb on + strand, within proCat 3498.108 kb on + strand, within proCat 3498.108 kb on + strand, within proCat 3498.116 kb on - strand, within proCat 3498.116 kb on - strand, within proCat 3498.178 kb on - strand, within proCat 3498.180 kb on + strand, within proCat 3498.210 kb on + strand, within proCat 3498.238 kb on - strand, within proCat 3498.255 kb on + strand, within proCat 3498.324 kb on + strand, within proCat 3498.346 kb on + strand, within proCat 3498.374 kb on - strand, within proCat 3498.457 kb on - strand, within proCat 3498.590 kb on + strand, within proCat 3498.696 kb on + strand, within proCat 3498.696 kb on + strand, within proCat 3498.704 kb on - strand, within proCat 3498.711 kb on - strand, within proCat 3498.716 kb on + strand, within proC

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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3,496,071 - +1.5
3,496,077 + +1.8
3,496,077 + +1.2
3,496,077 + +2.4
3,496,077 + +2.9
3,496,077 + +1.4
3,496,077 + +1.6
3,496,077 + +2.6
3,496,085 - +3.3
3,496,085 - +2.4
3,496,085 - -1.0
3,496,085 - +3.1
3,496,085 - +2.7
3,496,094 + +4.7
3,496,158 - pilT SO3351 0.16 +1.4
3,496,375 - pilT SO3351 0.37 -0.4
3,496,400 + pilT SO3351 0.39 +0.2
3,496,441 - pilT SO3351 0.43 +2.5
3,496,489 + pilT SO3351 0.48 +1.6
3,496,566 - pilT SO3351 0.55 -0.0
3,496,621 - pilT SO3351 0.61 +0.2
3,496,757 - pilT SO3351 0.74 -0.4
3,496,804 - pilT SO3351 0.78 +2.2
3,496,806 + pilT SO3351 0.79 -3.3
3,496,875 + pilT SO3351 0.85 -1.3
3,496,878 - pilT SO3351 0.85 +2.3
3,496,882 + pilT SO3351 0.86 +0.2
3,496,890 - pilT SO3351 0.87 +1.4
3,496,890 - pilT SO3351 0.87 +0.9
3,496,904 + pilT SO3351 0.88 +2.8
3,496,972 + +0.3
3,496,972 + +1.2
3,497,099 + -2.7
3,497,099 + -1.4
3,497,099 + -2.7
3,497,107 - -3.3
3,497,107 - -2.6
3,497,353 - SO3352 0.40 -0.4
3,497,545 - SO3352 0.67 -2.7
3,497,545 - SO3352 0.67 -0.4
3,497,545 - SO3352 0.67 -3.4
3,497,615 - SO3352 0.77 -4.7
3,497,793 + +1.1
3,497,954 + +0.6
3,497,974 + -0.3
3,497,974 + -1.6
3,497,974 + +0.4
3,497,974 + +0.6
3,497,982 - -3.8
3,498,034 + -2.6
3,498,063 + -1.7
3,498,094 + proC SO3354 0.11 -3.0
3,498,108 + proC SO3354 0.13 -3.4
3,498,108 + proC SO3354 0.13 -5.2
3,498,116 - proC SO3354 0.14 -2.4
3,498,116 - proC SO3354 0.14 -3.4
3,498,178 - proC SO3354 0.21 -1.4
3,498,180 + proC SO3354 0.22 -3.2
3,498,210 + proC SO3354 0.25 -4.0
3,498,238 - proC SO3354 0.29 -2.6
3,498,255 + proC SO3354 0.31 -1.4
3,498,324 + proC SO3354 0.39 -1.0
3,498,346 + proC SO3354 0.42 -2.1
3,498,374 - proC SO3354 0.45 -5.2
3,498,457 - proC SO3354 0.56 -4.2
3,498,590 + proC SO3354 0.72 -2.2
3,498,696 + proC SO3354 0.85 -5.3
3,498,696 + proC SO3354 0.85 -3.0
3,498,704 - proC SO3354 0.86 -2.0
3,498,711 - proC SO3354 0.87 -4.1
3,498,716 + proC SO3354 0.87 -2.7

Or see this region's nucleotide sequence