Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3322 and SO3323 are separated by 341 nucleotides SO3323 and SO3324 are separated by 63 nucleotides SO3324 and SO3325 are separated by 79 nucleotides SO3325 and SO3326 are separated by 126 nucleotides
SO3322: SO3322 - hypothetical protein (NCBI ptt file), at 3,474,097 to 3,474,417
SO3322
SO3323: SO3323 - acetyltransferase, GNAT family (NCBI ptt file), at 3,474,759 to 3,475,289
SO3323
SO3324: SO3324 - acetyltransferase, GNAT family (NCBI ptt file), at 3,475,353 to 3,475,964
SO3324
SO3325: SO3325 - nrfJ-related protein (NCBI ptt file), at 3,476,044 to 3,476,346
SO3325
SO3326: SO3326 - conserved hypothetical protein (NCBI ptt file), at 3,476,473 to 3,476,682
SO3326
Position (kb)
3475
3476 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3474.369 kb on - strand, within SO3322 at 3474.369 kb on - strand, within SO3322 at 3474.369 kb on - strand, within SO3322 at 3474.469 kb on - strand at 3474.607 kb on + strand at 3474.607 kb on + strand at 3474.637 kb on - strand at 3474.692 kb on + strand at 3474.829 kb on + strand, within SO3323 at 3474.864 kb on + strand, within SO3323 at 3474.878 kb on - strand, within SO3323 at 3474.954 kb on - strand, within SO3323 at 3474.963 kb on + strand, within SO3323 at 3474.963 kb on + strand, within SO3323 at 3475.054 kb on + strand, within SO3323 at 3475.061 kb on + strand, within SO3323 at 3475.061 kb on + strand, within SO3323 at 3475.061 kb on + strand, within SO3323 at 3475.061 kb on + strand, within SO3323 at 3475.061 kb on + strand, within SO3323 at 3475.061 kb on + strand, within SO3323 at 3475.069 kb on - strand, within SO3323 at 3475.069 kb on - strand, within SO3323 at 3475.069 kb on - strand, within SO3323 at 3475.069 kb on - strand, within SO3323 at 3475.069 kb on - strand, within SO3323 at 3475.069 kb on - strand, within SO3323 at 3475.092 kb on - strand, within SO3323 at 3475.107 kb on - strand, within SO3323 at 3475.144 kb on + strand, within SO3323 at 3475.203 kb on + strand, within SO3323 at 3475.220 kb on + strand, within SO3323 at 3475.253 kb on + strand at 3475.299 kb on - strand at 3475.302 kb on - strand at 3475.309 kb on - strand at 3475.309 kb on - strand at 3475.312 kb on + strand at 3475.474 kb on + strand, within SO3324 at 3475.484 kb on + strand, within SO3324 at 3475.485 kb on + strand, within SO3324 at 3475.491 kb on + strand, within SO3324 at 3475.492 kb on - strand, within SO3324 at 3475.492 kb on - strand, within SO3324 at 3475.492 kb on - strand, within SO3324 at 3475.493 kb on - strand, within SO3324 at 3475.493 kb on - strand, within SO3324 at 3475.507 kb on - strand, within SO3324 at 3475.507 kb on - strand, within SO3324 at 3475.536 kb on + strand, within SO3324 at 3475.542 kb on + strand, within SO3324 at 3475.750 kb on + strand, within SO3324 at 3475.775 kb on + strand, within SO3324 at 3475.875 kb on + strand, within SO3324 at 3475.932 kb on + strand at 3475.946 kb on - strand at 3475.956 kb on + strand at 3475.971 kb on + strand at 3475.971 kb on + strand at 3476.020 kb on + strand at 3476.020 kb on + strand at 3476.020 kb on + strand at 3476.028 kb on - strand at 3476.028 kb on - strand at 3476.091 kb on + strand, within SO3325 at 3476.245 kb on + strand, within SO3325 at 3476.310 kb on - strand, within SO3325 at 3476.342 kb on - strand at 3476.425 kb on + strand at 3476.425 kb on + strand at 3476.425 kb on + strand at 3476.425 kb on + strand at 3476.433 kb on - strand at 3476.433 kb on - strand at 3476.557 kb on - strand, within SO3326 at 3476.639 kb on + strand, within SO3326 at 3476.716 kb on + strand at 3476.761 kb on + strand at 3476.769 kb on - strand at 3476.769 kb on - strand at 3476.840 kb on - strand at 3476.850 kb on - strand at 3476.895 kb on - strand at 3476.895 kb on - strand at 3476.895 kb on - strand at 3476.920 kb on + strand at 3476.928 kb on - strand at 3476.928 kb on - strand at 3476.947 kb on + strand at 3476.955 kb on - strand at 3476.956 kb on + strand at 3476.957 kb on + strand at 3476.963 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,474,369 - SO3322 0.85 -1.6 3,474,369 - SO3322 0.85 +0.2 3,474,369 - SO3322 0.85 +0.6 3,474,469 - -1.9 3,474,607 + +2.2 3,474,607 + -0.4 3,474,637 - -1.4 3,474,692 + +0.1 3,474,829 + SO3323 0.13 -0.5 3,474,864 + SO3323 0.20 +2.8 3,474,878 - SO3323 0.22 +1.4 3,474,954 - SO3323 0.37 +0.6 3,474,963 + SO3323 0.38 +2.8 3,474,963 + SO3323 0.38 +0.8 3,475,054 + SO3323 0.56 +1.2 3,475,061 + SO3323 0.57 +2.3 3,475,061 + SO3323 0.57 -0.0 3,475,061 + SO3323 0.57 -0.5 3,475,061 + SO3323 0.57 +2.0 3,475,061 + SO3323 0.57 +2.3 3,475,061 + SO3323 0.57 +1.7 3,475,069 - SO3323 0.58 +1.2 3,475,069 - SO3323 0.58 +1.9 3,475,069 - SO3323 0.58 +1.6 3,475,069 - SO3323 0.58 -1.7 3,475,069 - SO3323 0.58 +1.8 3,475,069 - SO3323 0.58 +2.1 3,475,092 - SO3323 0.63 +2.6 3,475,107 - SO3323 0.66 +2.6 3,475,144 + SO3323 0.73 +4.5 3,475,203 + SO3323 0.84 +2.4 3,475,220 + SO3323 0.87 -0.0 3,475,253 + -4.2 3,475,299 - -2.2 3,475,302 - +0.8 3,475,309 - -2.2 3,475,309 - +2.0 3,475,312 + +2.3 3,475,474 + SO3324 0.20 -1.2 3,475,484 + SO3324 0.21 +5.4 3,475,485 + SO3324 0.22 +3.3 3,475,491 + SO3324 0.23 +4.0 3,475,492 - SO3324 0.23 -0.4 3,475,492 - SO3324 0.23 +2.4 3,475,492 - SO3324 0.23 +2.9 3,475,493 - SO3324 0.23 +2.8 3,475,493 - SO3324 0.23 +1.5 3,475,507 - SO3324 0.25 -2.7 3,475,507 - SO3324 0.25 +0.8 3,475,536 + SO3324 0.30 +1.1 3,475,542 + SO3324 0.31 +0.6 3,475,750 + SO3324 0.65 -0.2 3,475,775 + SO3324 0.69 +1.4 3,475,875 + SO3324 0.85 +0.9 3,475,932 + +0.6 3,475,946 - -1.4 3,475,956 + +1.0 3,475,971 + +1.8 3,475,971 + +1.2 3,476,020 + +2.1 3,476,020 + -1.0 3,476,020 + +1.3 3,476,028 - +0.1 3,476,028 - -1.2 3,476,091 + SO3325 0.16 -0.9 3,476,245 + SO3325 0.66 +0.6 3,476,310 - SO3325 0.88 -0.9 3,476,342 - +0.4 3,476,425 + -3.4 3,476,425 + -1.5 3,476,425 + -1.9 3,476,425 + +0.7 3,476,433 - -1.0 3,476,433 - +1.3 3,476,557 - SO3326 0.40 +1.6 3,476,639 + SO3326 0.79 -2.7 3,476,716 + -3.3 3,476,761 + +1.4 3,476,769 - -0.9 3,476,769 - -2.2 3,476,840 - +2.4 3,476,850 - -2.8 3,476,895 - -1.4 3,476,895 - +1.1 3,476,895 - -0.6 3,476,920 + +0.6 3,476,928 - -2.9 3,476,928 - -0.5 3,476,947 + +1.0 3,476,955 - +1.6 3,476,956 + -0.9 3,476,957 + +1.7 3,476,963 - +1.9
Or see this region's nucleotide sequence