Strain Fitness in Shewanella oneidensis MR-1 around SO3217

Experiment: L-Isoleucine (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	L-Isoleucine (C)
3,356,599	-	flhB	SO3215	0.87	+3.7
3,356,610	-	flhB	SO3215	0.88	+2.8
3,356,631	-				+1.8
3,356,692	+				+2.1
3,356,706	+				+3.6
3,356,748	+				+1.1
3,356,797	-				+0.1
3,356,828	-				+8.9
3,356,839	-				+2.1
3,356,839	-				+1.5
3,356,870	-	fliR	SO3216	0.13	+2.5
3,357,018	-	fliR	SO3216	0.31	+2.7
3,357,069	+	fliR	SO3216	0.38	+1.7
3,357,072	-	fliR	SO3216	0.38	+1.3
3,357,310	+	fliR	SO3216	0.68	-0.1
3,357,405	-	fliR	SO3216	0.80	+1.8
3,357,433	+	fliR	SO3216	0.83	+2.6
3,357,517	+				-1.1
3,357,524	-				+1.2
3,357,623	+	fliQ	SO3217	0.20	+2.2
3,357,623	+	fliQ	SO3217	0.20	+1.2
3,357,670	+	fliQ	SO3217	0.37	+3.7
3,357,697	-	fliQ	SO3217	0.47	+2.9
3,357,697	-	fliQ	SO3217	0.47	+2.3
3,357,744	+	fliQ	SO3217	0.65	+1.2
3,357,758	+	fliQ	SO3217	0.70	+1.8
3,357,826	+				+2.4
3,357,858	+				-0.1
3,357,861	-				-0.2
3,357,953	-	fliP	SO3218	0.13	+1.1
3,357,960	+	fliP	SO3218	0.14	+1.9
3,357,970	-	fliP	SO3218	0.15	+1.5
3,358,088	-	fliP	SO3218	0.31	-4.0
3,358,152	+	fliP	SO3218	0.40	+2.2
3,358,196	+	fliP	SO3218	0.46	-0.3
3,358,204	-	fliP	SO3218	0.47	-1.0
3,358,249	-	fliP	SO3218	0.53	+2.3
3,358,319	-	fliP	SO3218	0.62	+1.4
3,358,321	+	fliP	SO3218	0.62	-0.9
3,358,436	-	fliP	SO3218	0.78	+1.9
3,358,485	-	fliP	SO3218	0.85	+2.9
3,358,565	+				+0.6
3,358,594	-				+1.6
3,358,619	+				+1.2
3,358,757	+	fliO	SO3219	0.39	-1.3
3,358,757	+	fliO	SO3219	0.39	+3.2