Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheZ and cheY-3 are separated by 13 nucleotides cheY-3 and fliA are separated by 47 nucleotides fliA and flhG overlap by 8 nucleotides flhG and flhF are separated by 34 nucleotides
SO3208: cheZ - chemotaxis protein CheZ (NCBI ptt file), at 3,348,973 to 3,349,710
cheZ
SO3209: cheY-3 - chemotaxis protein CheY (NCBI ptt file), at 3,349,724 to 3,350,107
cheY-3
SO3210: fliA - RNA polymerase sigma-27 factor (NCBI ptt file), at 3,350,155 to 3,350,868
fliA
SO3211: flhG - flagellar biosynthetic protein FlhG (NCBI ptt file), at 3,350,861 to 3,351,712
flhG
SO3212: flhF - flagellar biosynthetic protein FlhF (NCBI ptt file), at 3,351,747 to 3,353,123
flhF
Position (kb)
3350
3351 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3349.302 kb on + strand, within cheZ at 3349.656 kb on - strand at 3349.666 kb on - strand at 3349.689 kb on + strand at 3349.712 kb on + strand at 3349.713 kb on - strand at 3349.720 kb on - strand at 3349.767 kb on - strand, within cheY-3 at 3349.789 kb on + strand, within cheY-3 at 3349.899 kb on + strand, within cheY-3 at 3350.007 kb on + strand, within cheY-3 at 3350.116 kb on - strand at 3350.191 kb on - strand at 3350.534 kb on - strand, within fliA at 3350.671 kb on - strand, within fliA at 3350.686 kb on - strand, within fliA at 3350.731 kb on - strand, within fliA at 3350.732 kb on + strand, within fliA at 3350.845 kb on + strand at 3350.853 kb on - strand at 3350.853 kb on - strand at 3350.853 kb on - strand at 3351.311 kb on - strand, within flhG at 3351.457 kb on - strand, within flhG at 3351.511 kb on - strand, within flhG at 3351.571 kb on - strand, within flhG at 3351.591 kb on + strand, within flhG at 3351.638 kb on + strand at 3351.646 kb on - strand at 3351.770 kb on - strand at 3351.770 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,349,302 + cheZ SO3208 0.45 -3.0 3,349,656 - -0.8 3,349,666 - +2.3 3,349,689 + -1.1 3,349,712 + -0.1 3,349,713 - -1.5 3,349,720 - +1.2 3,349,767 - cheY-3 SO3209 0.11 -0.0 3,349,789 + cheY-3 SO3209 0.17 +2.1 3,349,899 + cheY-3 SO3209 0.46 +0.5 3,350,007 + cheY-3 SO3209 0.74 -0.8 3,350,116 - +0.1 3,350,191 - -1.1 3,350,534 - fliA SO3210 0.53 +2.0 3,350,671 - fliA SO3210 0.72 +1.9 3,350,686 - fliA SO3210 0.74 +2.5 3,350,731 - fliA SO3210 0.81 +3.4 3,350,732 + fliA SO3210 0.81 +3.8 3,350,845 + +3.8 3,350,853 - +4.8 3,350,853 - +2.4 3,350,853 - +1.5 3,351,311 - flhG SO3211 0.53 -4.3 3,351,457 - flhG SO3211 0.70 -4.3 3,351,511 - flhG SO3211 0.76 -1.8 3,351,571 - flhG SO3211 0.83 -3.7 3,351,591 + flhG SO3211 0.86 -0.5 3,351,638 + -1.5 3,351,646 - -3.5 3,351,770 - -2.0 3,351,770 - -0.5
Or see this region's nucleotide sequence