Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt etfA and etfB are separated by 0 nucleotides etfB and SO3146 are separated by 310 nucleotides SO3146 and SO3147 are separated by 163 nucleotides
SO3144: etfA - electron transfer flavoprotein, alpha subunit (NCBI ptt file), at 3,280,752 to 3,281,678
etfA
SO3145: etfB - electron transfer flavoprotein, beta subunit (NCBI ptt file), at 3,281,679 to 3,282,428
etfB
SO3146: SO3146 - DNA-binding protein, H-NS family (NCBI ptt file), at 3,282,739 to 3,283,131
SO3146
SO3147: SO3147 - hypothetical protein (NCBI ptt file), at 3,283,295 to 3,283,390
SO3147
Position (kb)
3281
3282
3283 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 3280.679 kb on - strand at 3280.704 kb on - strand at 3280.706 kb on - strand at 3280.881 kb on - strand, within etfA at 3280.898 kb on - strand, within etfA at 3281.114 kb on + strand, within etfA at 3281.117 kb on - strand, within etfA at 3281.117 kb on - strand at 3281.195 kb on - strand, within etfA at 3281.242 kb on + strand, within etfA at 3281.315 kb on - strand, within etfA at 3281.322 kb on + strand, within etfA at 3281.431 kb on - strand, within etfA at 3281.516 kb on - strand, within etfA at 3281.547 kb on - strand, within etfA at 3281.556 kb on + strand, within etfA at 3281.559 kb on - strand, within etfA at 3281.611 kb on + strand at 3281.735 kb on + strand at 3281.743 kb on - strand at 3281.746 kb on + strand at 3281.746 kb on + strand at 3281.761 kb on + strand, within etfB at 3281.773 kb on - strand at 3281.778 kb on - strand, within etfB at 3281.838 kb on - strand, within etfB at 3281.879 kb on - strand, within etfB at 3281.925 kb on - strand, within etfB at 3281.937 kb on + strand, within etfB at 3281.937 kb on - strand, within etfB at 3281.939 kb on + strand, within etfB at 3281.942 kb on - strand, within etfB at 3282.039 kb on + strand, within etfB at 3282.047 kb on - strand, within etfB at 3282.166 kb on + strand, within etfB at 3282.186 kb on + strand, within etfB at 3282.193 kb on + strand, within etfB at 3282.197 kb on + strand, within etfB at 3282.249 kb on + strand, within etfB at 3282.257 kb on + strand, within etfB at 3282.268 kb on - strand, within etfB at 3282.331 kb on + strand, within etfB at 3282.331 kb on + strand, within etfB at 3282.357 kb on + strand at 3282.367 kb on + strand at 3282.367 kb on + strand at 3282.367 kb on + strand at 3282.368 kb on + strand at 3282.376 kb on - strand at 3282.410 kb on - strand at 3282.413 kb on - strand at 3282.422 kb on - strand at 3282.436 kb on + strand at 3282.472 kb on - strand at 3282.480 kb on - strand at 3282.487 kb on + strand at 3282.490 kb on - strand at 3282.490 kb on - strand at 3282.490 kb on - strand at 3282.497 kb on + strand at 3282.506 kb on - strand at 3282.507 kb on + strand at 3282.514 kb on + strand at 3282.515 kb on - strand at 3282.515 kb on - strand at 3282.515 kb on - strand at 3282.515 kb on - strand at 3282.522 kb on - strand at 3282.522 kb on - strand at 3282.539 kb on - strand at 3282.569 kb on - strand at 3282.576 kb on + strand at 3282.584 kb on - strand at 3282.584 kb on - strand at 3282.607 kb on - strand at 3282.682 kb on + strand at 3282.698 kb on + strand at 3282.698 kb on + strand at 3282.706 kb on - strand at 3282.777 kb on + strand at 3282.806 kb on + strand, within SO3146 at 3282.806 kb on + strand, within SO3146 at 3282.811 kb on - strand, within SO3146 at 3282.816 kb on + strand, within SO3146 at 3282.828 kb on + strand, within SO3146 at 3282.864 kb on + strand, within SO3146 at 3282.869 kb on + strand, within SO3146 at 3282.885 kb on + strand, within SO3146 at 3282.886 kb on + strand, within SO3146 at 3282.887 kb on + strand, within SO3146 at 3282.930 kb on + strand, within SO3146 at 3282.952 kb on + strand, within SO3146 at 3283.126 kb on + strand at 3283.131 kb on + strand at 3283.139 kb on - strand at 3283.146 kb on + strand at 3283.146 kb on + strand at 3283.154 kb on - strand at 3283.270 kb on - strand at 3283.277 kb on + strand at 3283.277 kb on + strand at 3283.285 kb on - strand at 3283.285 kb on - strand at 3283.285 kb on - strand at 3283.287 kb on + strand at 3283.287 kb on + strand at 3283.287 kb on + strand at 3283.287 kb on + strand at 3283.287 kb on + strand at 3283.292 kb on + strand at 3283.295 kb on - strand at 3283.295 kb on - strand at 3283.308 kb on - strand, within SO3147 at 3283.310 kb on + strand, within SO3147 at 3283.312 kb on - strand, within SO3147 at 3283.316 kb on - strand, within SO3147 at 3283.367 kb on + strand, within SO3147 at 3283.405 kb on + strand at 3283.413 kb on - strand at 3283.413 kb on - strand at 3283.413 kb on - strand at 3283.413 kb on - strand at 3283.424 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,280,679 - +1.3 3,280,704 - -0.2 3,280,706 - -2.1 3,280,881 - etfA SO3144 0.14 -3.1 3,280,898 - etfA SO3144 0.16 -3.3 3,281,114 + etfA SO3144 0.39 -4.2 3,281,117 - etfA SO3144 0.39 -4.3 3,281,117 - +0.5 3,281,195 - etfA SO3144 0.48 -1.1 3,281,242 + etfA SO3144 0.53 -2.9 3,281,315 - etfA SO3144 0.61 -1.8 3,281,322 + etfA SO3144 0.61 -5.0 3,281,431 - etfA SO3144 0.73 -2.4 3,281,516 - etfA SO3144 0.82 -2.9 3,281,547 - etfA SO3144 0.86 -2.9 3,281,556 + etfA SO3144 0.87 -4.1 3,281,559 - etfA SO3144 0.87 -4.8 3,281,611 + -4.4 3,281,735 + -2.1 3,281,743 - -4.6 3,281,746 + -1.9 3,281,746 + -2.9 3,281,761 + etfB SO3145 0.11 -1.5 3,281,773 - +0.5 3,281,778 - etfB SO3145 0.13 -2.9 3,281,838 - etfB SO3145 0.21 -2.3 3,281,879 - etfB SO3145 0.27 -1.5 3,281,925 - etfB SO3145 0.33 -5.6 3,281,937 + etfB SO3145 0.34 -3.9 3,281,937 - etfB SO3145 0.34 -5.1 3,281,939 + etfB SO3145 0.35 -2.1 3,281,942 - etfB SO3145 0.35 -3.7 3,282,039 + etfB SO3145 0.48 -4.7 3,282,047 - etfB SO3145 0.49 -3.4 3,282,166 + etfB SO3145 0.65 -0.5 3,282,186 + etfB SO3145 0.68 -4.5 3,282,193 + etfB SO3145 0.69 -2.9 3,282,197 + etfB SO3145 0.69 -3.1 3,282,249 + etfB SO3145 0.76 -4.3 3,282,257 + etfB SO3145 0.77 -3.6 3,282,268 - etfB SO3145 0.79 -2.7 3,282,331 + etfB SO3145 0.87 -4.3 3,282,331 + etfB SO3145 0.87 -3.8 3,282,357 + -2.5 3,282,367 + -3.2 3,282,367 + -1.1 3,282,367 + -2.9 3,282,368 + -2.1 3,282,376 - -4.6 3,282,410 - -2.8 3,282,413 - -6.1 3,282,422 - -1.5 3,282,436 + -4.8 3,282,472 - -2.3 3,282,480 - -1.9 3,282,487 + -1.1 3,282,490 - -5.2 3,282,490 - -3.1 3,282,490 - -2.7 3,282,497 + -3.9 3,282,506 - -4.3 3,282,507 + -3.6 3,282,514 + -3.9 3,282,515 - -3.2 3,282,515 - -4.4 3,282,515 - -1.5 3,282,515 - -5.1 3,282,522 - -0.5 3,282,522 - -3.2 3,282,539 - -3.2 3,282,569 - -2.3 3,282,576 + +0.2 3,282,584 - +0.5 3,282,584 - -0.9 3,282,607 - +0.7 3,282,682 + -0.5 3,282,698 + -2.3 3,282,698 + -1.8 3,282,706 - -2.9 3,282,777 + -2.6 3,282,806 + SO3146 0.17 -3.1 3,282,806 + SO3146 0.17 -2.3 3,282,811 - SO3146 0.18 -0.1 3,282,816 + SO3146 0.20 -3.4 3,282,828 + SO3146 0.23 -1.5 3,282,864 + SO3146 0.32 -2.9 3,282,869 + SO3146 0.33 -0.8 3,282,885 + SO3146 0.37 -2.9 3,282,886 + SO3146 0.37 -2.3 3,282,887 + SO3146 0.38 -1.5 3,282,930 + SO3146 0.49 -3.1 3,282,952 + SO3146 0.54 +0.5 3,283,126 + -2.6 3,283,131 + +0.1 3,283,139 - +1.8 3,283,146 + -0.5 3,283,146 + -1.9 3,283,154 - +0.2 3,283,270 - +2.0 3,283,277 + +1.8 3,283,277 + +0.9 3,283,285 - +1.2 3,283,285 - +2.8 3,283,285 - +1.1 3,283,287 + +1.1 3,283,287 + -0.2 3,283,287 + +0.3 3,283,287 + +1.8 3,283,287 + +2.3 3,283,292 + +1.7 3,283,295 - +0.8 3,283,295 - +3.1 3,283,308 - SO3147 0.14 -0.1 3,283,310 + SO3147 0.16 +3.9 3,283,312 - SO3147 0.18 +1.9 3,283,316 - SO3147 0.22 +1.5 3,283,367 + SO3147 0.75 +1.7 3,283,405 + +2.2 3,283,413 - +4.5 3,283,413 - -0.3 3,283,413 - -0.2 3,283,413 - +0.5 3,283,424 + +2.1
Or see this region's nucleotide sequence