Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fabB and SO3073 are separated by 306 nucleotides SO3073 and SO3075 are separated by 194 nucleotides SO3075 and SO3076 are separated by 0 nucleotides
SO3072: fabB - 3-oxoacyl-(acyl-carrier-protein) synthase I (NCBI ptt file), at 3,191,404 to 3,192,615
fabB
SO3073: SO3073 - conserved hypothetical protein (NCBI ptt file), at 3,192,922 to 3,194,853
SO3073
SO3075: SO3075 - RNA methyltransferase, TrmH family, putative (NCBI ptt file), at 3,195,048 to 3,195,659
SO3075
SO3076: SO3076 - conserved hypothetical protein (NCBI ptt file), at 3,195,660 to 3,195,941
SO3076
Position (kb)
3192
3193
3194
3195 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3192.642 kb on - strand at 3192.815 kb on - strand at 3192.838 kb on + strand at 3192.846 kb on - strand at 3192.892 kb on + strand at 3192.903 kb on + strand at 3192.928 kb on + strand at 3192.945 kb on - strand at 3193.145 kb on - strand, within SO3073 at 3193.161 kb on - strand, within SO3073 at 3193.227 kb on - strand, within SO3073 at 3193.227 kb on - strand, within SO3073 at 3193.364 kb on + strand, within SO3073 at 3193.372 kb on - strand, within SO3073 at 3193.528 kb on + strand, within SO3073 at 3193.550 kb on + strand, within SO3073 at 3193.590 kb on + strand, within SO3073 at 3193.611 kb on + strand, within SO3073 at 3193.626 kb on + strand, within SO3073 at 3193.656 kb on - strand, within SO3073 at 3193.663 kb on - strand, within SO3073 at 3193.760 kb on - strand, within SO3073 at 3193.900 kb on - strand, within SO3073 at 3193.915 kb on - strand, within SO3073 at 3194.072 kb on - strand, within SO3073 at 3194.079 kb on + strand, within SO3073 at 3194.079 kb on + strand, within SO3073 at 3194.087 kb on - strand, within SO3073 at 3194.087 kb on - strand, within SO3073 at 3194.154 kb on + strand, within SO3073 at 3194.171 kb on + strand, within SO3073 at 3194.227 kb on - strand, within SO3073 at 3194.346 kb on + strand, within SO3073 at 3194.357 kb on + strand, within SO3073 at 3194.554 kb on + strand, within SO3073 at 3194.594 kb on + strand, within SO3073 at 3194.619 kb on + strand, within SO3073 at 3194.791 kb on - strand at 3194.848 kb on + strand at 3194.896 kb on + strand at 3195.019 kb on - strand at 3195.040 kb on + strand at 3195.040 kb on + strand at 3195.048 kb on - strand at 3195.107 kb on - strand at 3195.188 kb on + strand, within SO3075 at 3195.193 kb on + strand, within SO3075 at 3195.196 kb on - strand, within SO3075 at 3195.196 kb on - strand, within SO3075 at 3195.454 kb on + strand, within SO3075 at 3195.717 kb on - strand, within SO3076 at 3195.743 kb on - strand, within SO3076 at 3195.806 kb on - strand, within SO3076
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,192,642 - +0.0 3,192,815 - -0.3 3,192,838 + +0.2 3,192,846 - +1.0 3,192,892 + -0.2 3,192,903 + +3.7 3,192,928 + -0.1 3,192,945 - +0.4 3,193,145 - SO3073 0.12 +0.4 3,193,161 - SO3073 0.12 -1.7 3,193,227 - SO3073 0.16 -1.6 3,193,227 - SO3073 0.16 +0.8 3,193,364 + SO3073 0.23 -2.0 3,193,372 - SO3073 0.23 -1.0 3,193,528 + SO3073 0.31 -3.1 3,193,550 + SO3073 0.33 -0.2 3,193,590 + SO3073 0.35 -0.2 3,193,611 + SO3073 0.36 -2.2 3,193,626 + SO3073 0.36 +1.1 3,193,656 - SO3073 0.38 -1.0 3,193,663 - SO3073 0.38 -0.3 3,193,760 - SO3073 0.43 -0.7 3,193,900 - SO3073 0.51 -0.1 3,193,915 - SO3073 0.51 -0.1 3,194,072 - SO3073 0.60 +1.4 3,194,079 + SO3073 0.60 +0.3 3,194,079 + SO3073 0.60 -1.4 3,194,087 - SO3073 0.60 +0.7 3,194,087 - SO3073 0.60 +0.0 3,194,154 + SO3073 0.64 +0.7 3,194,171 + SO3073 0.65 -0.4 3,194,227 - SO3073 0.68 +0.6 3,194,346 + SO3073 0.74 -0.8 3,194,357 + SO3073 0.74 +1.8 3,194,554 + SO3073 0.84 +0.1 3,194,594 + SO3073 0.87 -1.6 3,194,619 + SO3073 0.88 +1.8 3,194,791 - -1.6 3,194,848 + +0.9 3,194,896 + -1.5 3,195,019 - -0.5 3,195,040 + -0.4 3,195,040 + +2.0 3,195,048 - -2.2 3,195,107 - +0.4 3,195,188 + SO3075 0.23 -0.3 3,195,193 + SO3075 0.24 +0.3 3,195,196 - SO3075 0.24 +0.8 3,195,196 - SO3075 0.24 +0.1 3,195,454 + SO3075 0.66 -0.3 3,195,717 - SO3076 0.20 -1.7 3,195,743 - SO3076 0.29 -2.9 3,195,806 - SO3076 0.52 -1.0
Or see this region's nucleotide sequence