Strain Fitness in Shewanella oneidensis MR-1 around SO3071

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntasd and pdxB are separated by 2 nucleotidespdxB and fabB are separated by 146 nucleotides SO3070: asd - aspartate semialdehyde dehydrogenese (NCBI ptt file), at 3,189,108 to 3,190,124 asd SO3071: pdxB - erythronate-4-phosphate dehydrogenase (NCBI ptt file), at 3,190,127 to 3,191,257 pdxB SO3072: fabB - 3-oxoacyl-(acyl-carrier-protein) synthase I (NCBI ptt file), at 3,191,404 to 3,192,615 fabB Position (kb) 3190 3191 3192Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 3190.193 kb on - strandat 3190.193 kb on - strandat 3191.329 kb on + strandat 3191.329 kb on + strandat 3191.330 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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3,190,193 - +4.2
3,190,193 - +6.5
3,191,329 + -0.2
3,191,329 + -3.2
3,191,330 - +0.6

Or see this region's nucleotide sequence