Strain Fitness in Shewanella oneidensis MR-1 around SO3030

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO3029 and alcA are separated by 576 nucleotidesalcA and SO3031 overlap by 1 nucleotidesSO3031 and SO3032 are separated by 111 nucleotides SO3029: SO3029 - hypothetical protein (NCBI ptt file), at 3,139,145 to 3,139,429 SO3029 SO3030: alcA - siderophore biosynthesis protein (NCBI ptt file), at 3,140,006 to 3,141,502 alcA SO3031: SO3031 - siderophore biosynthesis protein, putative (NCBI ptt file), at 3,141,502 to 3,142,122 SO3031 SO3032: SO3032 - siderophore biosynthesis protein, putative (NCBI ptt file), at 3,142,234 to 3,144,039 SO3032 Position (kb) 3140 3141 3142Strain fitness (log2 ratio) -2 -1 0 1 2at 3139.131 kb on + strandat 3139.131 kb on - strandat 3139.131 kb on - strandat 3139.160 kb on - strandat 3139.310 kb on - strand, within SO3029at 3139.599 kb on - strandat 3139.766 kb on - strandat 3139.910 kb on - strandat 3139.948 kb on - strandat 3139.950 kb on - strandat 3140.000 kb on + strandat 3140.060 kb on + strandat 3140.076 kb on + strandat 3140.084 kb on - strandat 3140.095 kb on + strandat 3140.111 kb on + strandat 3140.160 kb on + strand, within alcAat 3140.276 kb on + strand, within alcAat 3140.284 kb on - strand, within alcAat 3140.448 kb on + strand, within alcAat 3140.456 kb on + strand, within alcAat 3140.626 kb on - strand, within alcAat 3140.813 kb on + strand, within alcAat 3140.912 kb on + strand, within alcAat 3140.980 kb on + strand, within alcAat 3141.031 kb on - strand, within alcAat 3141.191 kb on - strand, within alcAat 3141.250 kb on - strand, within alcAat 3141.577 kb on + strand, within SO3031at 3141.577 kb on + strand, within SO3031at 3141.769 kb on - strand, within SO3031at 3141.820 kb on + strand, within SO3031at 3142.116 kb on + strandat 3142.330 kb on + strandat 3142.376 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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3,139,131 + -0.8
3,139,131 - +2.1
3,139,131 - +0.2
3,139,160 - -1.8
3,139,310 - SO3029 0.58 +0.7
3,139,599 - +2.2
3,139,766 - +0.5
3,139,910 - -1.4
3,139,948 - -2.2
3,139,950 - +2.1
3,140,000 + -0.2
3,140,060 + +1.7
3,140,076 + +2.6
3,140,084 - +2.2
3,140,095 + +2.1
3,140,111 + +0.9
3,140,160 + alcA SO3030 0.10 +1.9
3,140,276 + alcA SO3030 0.18 +0.6
3,140,284 - alcA SO3030 0.19 +2.0
3,140,448 + alcA SO3030 0.30 +1.5
3,140,456 + alcA SO3030 0.30 +1.3
3,140,626 - alcA SO3030 0.41 +2.7
3,140,813 + alcA SO3030 0.54 +0.9
3,140,912 + alcA SO3030 0.61 +1.0
3,140,980 + alcA SO3030 0.65 +1.4
3,141,031 - alcA SO3030 0.68 +1.8
3,141,191 - alcA SO3030 0.79 +1.0
3,141,250 - alcA SO3030 0.83 +2.2
3,141,577 + SO3031 0.12 -1.2
3,141,577 + SO3031 0.12 +1.4
3,141,769 - SO3031 0.43 +1.8
3,141,820 + SO3031 0.51 +1.3
3,142,116 + +0.6
3,142,330 + -0.1
3,142,376 + +1.0

Or see this region's nucleotide sequence