Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3013 and SO3014 are separated by 181 nucleotides SO3014 and SO3015 are separated by 3 nucleotides SO3015 and SO3016 are separated by 189 nucleotides SO3016 and SO3017 overlap by 4 nucleotides
SO3013: SO3013 - site-specific recombinase, phage integrase family (NCBI ptt file), at 3,124,990 to 3,126,249
SO3013
SO3014: SO3014 - conserved hypothetical protein (NCBI ptt file), at 3,126,431 to 3,127,027
SO3014
SO3015: SO3015 - conserved hypothetical protein (NCBI ptt file), at 3,127,031 to 3,127,819
SO3015
SO3016: SO3016 - Sua5/YciO/YrdC/YwlC family protein (NCBI ptt file), at 3,128,009 to 3,128,629
SO3016
SO3017: SO3017 - TrpH family protein (NCBI ptt file), at 3,128,626 to 3,129,486
SO3017
Position (kb)
3127
3128 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3126.035 kb on + strand, within SO3013 at 3126.035 kb on + strand, within SO3013 at 3126.043 kb on - strand, within SO3013 at 3126.043 kb on - strand, within SO3013 at 3126.053 kb on - strand, within SO3013 at 3126.053 kb on - strand, within SO3013 at 3126.057 kb on + strand, within SO3013 at 3126.082 kb on - strand, within SO3013 at 3126.082 kb on - strand, within SO3013 at 3126.082 kb on - strand, within SO3013 at 3126.170 kb on - strand at 3126.229 kb on - strand at 3126.283 kb on - strand at 3126.458 kb on + strand at 3126.468 kb on - strand at 3126.601 kb on - strand, within SO3014 at 3126.720 kb on - strand, within SO3014 at 3126.735 kb on - strand, within SO3014 at 3126.963 kb on + strand, within SO3014 at 3126.963 kb on + strand, within SO3014 at 3126.972 kb on + strand at 3127.008 kb on - strand at 3127.063 kb on + strand at 3127.068 kb on + strand at 3127.111 kb on + strand, within SO3015 at 3127.434 kb on + strand, within SO3015 at 3127.435 kb on + strand, within SO3015 at 3127.478 kb on - strand, within SO3015 at 3127.845 kb on + strand at 3127.845 kb on + strand at 3127.845 kb on + strand at 3127.845 kb on + strand at 3127.853 kb on - strand at 3127.978 kb on + strand at 3127.978 kb on + strand at 3127.978 kb on + strand at 3127.978 kb on + strand at 3127.986 kb on - strand at 3127.998 kb on - strand at 3128.029 kb on + strand at 3128.168 kb on - strand, within SO3016 at 3128.234 kb on - strand, within SO3016 at 3128.244 kb on + strand, within SO3016 at 3128.344 kb on + strand, within SO3016 at 3128.352 kb on - strand, within SO3016 at 3128.352 kb on - strand, within SO3016 at 3128.352 kb on - strand, within SO3016 at 3128.389 kb on + strand, within SO3016 at 3128.513 kb on + strand, within SO3016 at 3128.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,126,035 + SO3013 0.83 +1.4 3,126,035 + SO3013 0.83 -0.6 3,126,043 - SO3013 0.84 -0.7 3,126,043 - SO3013 0.84 -3.6 3,126,053 - SO3013 0.84 +1.0 3,126,053 - SO3013 0.84 -1.8 3,126,057 + SO3013 0.85 -0.9 3,126,082 - SO3013 0.87 -3.0 3,126,082 - SO3013 0.87 +0.8 3,126,082 - SO3013 0.87 -1.0 3,126,170 - +1.4 3,126,229 - +1.9 3,126,283 - -3.1 3,126,458 + -3.4 3,126,468 - +0.2 3,126,601 - SO3014 0.28 -1.7 3,126,720 - SO3014 0.48 +0.1 3,126,735 - SO3014 0.51 +0.4 3,126,963 + SO3014 0.89 -1.3 3,126,963 + SO3014 0.89 -2.4 3,126,972 + -2.2 3,127,008 - -0.0 3,127,063 + -1.3 3,127,068 + +2.4 3,127,111 + SO3015 0.10 +0.4 3,127,434 + SO3015 0.51 -2.2 3,127,435 + SO3015 0.51 -1.6 3,127,478 - SO3015 0.57 -0.3 3,127,845 + -0.8 3,127,845 + -2.6 3,127,845 + -0.9 3,127,845 + +0.6 3,127,853 - -0.7 3,127,978 + +1.1 3,127,978 + +2.9 3,127,978 + +1.7 3,127,978 + +0.8 3,127,986 - +0.4 3,127,998 - +0.4 3,128,029 + +0.2 3,128,168 - SO3016 0.26 -4.0 3,128,234 - SO3016 0.36 -1.1 3,128,244 + SO3016 0.38 -2.4 3,128,344 + SO3016 0.54 -0.6 3,128,352 - SO3016 0.55 +0.9 3,128,352 - SO3016 0.55 +0.3 3,128,352 - SO3016 0.55 +0.6 3,128,389 + SO3016 0.61 +2.8 3,128,513 + SO3016 0.81 +2.9 3,128,598 - +0.7
Or see this region's nucleotide sequence