Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt zipA and SO2898 are separated by 26 nucleotides SO2898 and cysZ are separated by 240 nucleotides
SO2897: zipA - cell division protein ZipA (NCBI ptt file), at 3,019,919 to 3,020,953
zipA
SO2898: SO2898 - SMC family protein (NCBI ptt file), at 3,020,980 to 3,024,417
SO2898
SO2899: cysZ - cysZ protein (NCBI ptt file), at 3,024,658 to 3,025,437
cysZ
Position (kb)
3020
3021
3022
3023
3024
3025 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3020.984 kb on - strand at 3021.133 kb on - strand at 3021.180 kb on - strand at 3021.375 kb on - strand, within SO2898 at 3021.579 kb on + strand, within SO2898 at 3021.899 kb on - strand, within SO2898 at 3022.038 kb on + strand, within SO2898 at 3022.215 kb on + strand, within SO2898 at 3022.239 kb on + strand, within SO2898 at 3022.267 kb on + strand, within SO2898 at 3022.425 kb on + strand, within SO2898 at 3022.434 kb on - strand, within SO2898 at 3022.599 kb on - strand, within SO2898 at 3022.609 kb on - strand, within SO2898 at 3022.747 kb on - strand, within SO2898 at 3022.759 kb on + strand, within SO2898 at 3022.759 kb on + strand, within SO2898 at 3022.793 kb on - strand, within SO2898 at 3022.822 kb on - strand, within SO2898 at 3022.894 kb on + strand, within SO2898 at 3022.948 kb on + strand, within SO2898 at 3023.157 kb on - strand, within SO2898 at 3023.280 kb on + strand, within SO2898 at 3023.484 kb on + strand, within SO2898 at 3023.484 kb on + strand, within SO2898 at 3023.492 kb on - strand, within SO2898 at 3023.492 kb on - strand, within SO2898 at 3023.492 kb on - strand, within SO2898 at 3023.595 kb on + strand, within SO2898 at 3023.600 kb on + strand, within SO2898 at 3023.603 kb on - strand, within SO2898 at 3023.660 kb on + strand, within SO2898 at 3023.660 kb on + strand, within SO2898 at 3023.660 kb on - strand, within SO2898 at 3023.777 kb on + strand, within SO2898 at 3023.777 kb on + strand, within SO2898 at 3023.777 kb on + strand, within SO2898 at 3023.785 kb on - strand, within SO2898 at 3023.980 kb on - strand, within SO2898 at 3024.001 kb on - strand, within SO2898 at 3024.006 kb on + strand, within SO2898 at 3024.006 kb on + strand, within SO2898 at 3024.014 kb on - strand, within SO2898 at 3024.014 kb on - strand, within SO2898 at 3024.014 kb on - strand, within SO2898 at 3024.120 kb on + strand at 3024.241 kb on - strand at 3024.389 kb on - strand at 3024.467 kb on + strand at 3024.485 kb on - strand at 3024.486 kb on - strand at 3024.604 kb on - strand at 3024.614 kb on + strand at 3024.621 kb on + strand at 3024.687 kb on + strand at 3024.687 kb on + strand at 3024.687 kb on + strand at 3024.687 kb on + strand at 3024.695 kb on - strand at 3024.696 kb on - strand at 3024.742 kb on + strand, within cysZ at 3024.742 kb on + strand, within cysZ at 3024.750 kb on - strand, within cysZ at 3024.817 kb on + strand, within cysZ at 3024.893 kb on + strand, within cysZ at 3024.893 kb on + strand, within cysZ at 3024.896 kb on - strand, within cysZ at 3024.908 kb on + strand, within cysZ at 3024.975 kb on - strand, within cysZ at 3025.010 kb on - strand, within cysZ at 3025.010 kb on - strand, within cysZ at 3025.013 kb on - strand, within cysZ at 3025.044 kb on - strand, within cysZ at 3025.272 kb on - strand, within cysZ at 3025.289 kb on + strand, within cysZ at 3025.309 kb on - strand, within cysZ at 3025.406 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,020,984 - +2.1 3,021,133 - -1.4 3,021,180 - +1.0 3,021,375 - SO2898 0.11 -0.5 3,021,579 + SO2898 0.17 +0.4 3,021,899 - SO2898 0.27 -0.3 3,022,038 + SO2898 0.31 -2.2 3,022,215 + SO2898 0.36 -1.5 3,022,239 + SO2898 0.37 -0.2 3,022,267 + SO2898 0.37 -1.8 3,022,425 + SO2898 0.42 -0.8 3,022,434 - SO2898 0.42 -4.4 3,022,599 - SO2898 0.47 -1.5 3,022,609 - SO2898 0.47 +1.4 3,022,747 - SO2898 0.51 -2.2 3,022,759 + SO2898 0.52 -2.2 3,022,759 + SO2898 0.52 -1.3 3,022,793 - SO2898 0.53 -0.9 3,022,822 - SO2898 0.54 -1.8 3,022,894 + SO2898 0.56 -3.2 3,022,948 + SO2898 0.57 -0.7 3,023,157 - SO2898 0.63 -1.9 3,023,280 + SO2898 0.67 -1.5 3,023,484 + SO2898 0.73 -2.2 3,023,484 + SO2898 0.73 -2.6 3,023,492 - SO2898 0.73 +0.6 3,023,492 - SO2898 0.73 +0.2 3,023,492 - SO2898 0.73 -0.1 3,023,595 + SO2898 0.76 -3.1 3,023,600 + SO2898 0.76 -0.3 3,023,603 - SO2898 0.76 -1.0 3,023,660 + SO2898 0.78 -2.7 3,023,660 + SO2898 0.78 -1.3 3,023,660 - SO2898 0.78 +0.4 3,023,777 + SO2898 0.81 -2.2 3,023,777 + SO2898 0.81 -3.6 3,023,777 + SO2898 0.81 -2.2 3,023,785 - SO2898 0.82 -0.6 3,023,980 - SO2898 0.87 -1.5 3,024,001 - SO2898 0.88 -1.6 3,024,006 + SO2898 0.88 +0.0 3,024,006 + SO2898 0.88 -0.3 3,024,014 - SO2898 0.88 +0.5 3,024,014 - SO2898 0.88 -1.3 3,024,014 - SO2898 0.88 -0.7 3,024,120 + -1.9 3,024,241 - -1.2 3,024,389 - -0.6 3,024,467 + -2.8 3,024,485 - +1.7 3,024,486 - -1.3 3,024,604 - +3.6 3,024,614 + -3.7 3,024,621 + -1.5 3,024,687 + -0.2 3,024,687 + +0.2 3,024,687 + +0.9 3,024,687 + -0.2 3,024,695 - +1.7 3,024,696 - -1.3 3,024,742 + cysZ SO2899 0.11 -0.2 3,024,742 + cysZ SO2899 0.11 -1.3 3,024,750 - cysZ SO2899 0.12 -0.1 3,024,817 + cysZ SO2899 0.20 -0.9 3,024,893 + cysZ SO2899 0.30 -0.7 3,024,893 + cysZ SO2899 0.30 -2.2 3,024,896 - cysZ SO2899 0.31 +0.4 3,024,908 + cysZ SO2899 0.32 -0.6 3,024,975 - cysZ SO2899 0.41 -1.3 3,025,010 - cysZ SO2899 0.45 -1.3 3,025,010 - cysZ SO2899 0.45 -1.6 3,025,013 - cysZ SO2899 0.46 +0.1 3,025,044 - cysZ SO2899 0.49 -1.9 3,025,272 - cysZ SO2899 0.79 -1.0 3,025,289 + cysZ SO2899 0.81 -3.4 3,025,309 - cysZ SO2899 0.83 +0.3 3,025,406 - +2.1
Or see this region's nucleotide sequence