Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhaB and dsbB are separated by 97 nucleotides dsbB and SO2888 are separated by 23 nucleotides SO2888 and SO2889 are separated by 320 nucleotides
SO2886: nhaB - Na+/H+ antiporter (NCBI ptt file), at 3,008,481 to 3,010,082
nhaB
SO2887: dsbB - disulfide bond formation protein b (NCBI ptt file), at 3,010,180 to 3,010,707
dsbB
SO2888: SO2888 - hypothetical protein (NCBI ptt file), at 3,010,731 to 3,011,000
SO2888
SO2889: SO2889 - sensory box histidine kinase (NCBI ptt file), at 3,011,321 to 3,013,930
SO2889
Position (kb)
3010
3011 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3009.240 kb on + strand, within nhaB at 3009.325 kb on - strand, within nhaB at 3009.339 kb on - strand, within nhaB at 3009.410 kb on - strand, within nhaB at 3009.441 kb on + strand, within nhaB at 3009.449 kb on - strand, within nhaB at 3009.454 kb on - strand, within nhaB at 3009.462 kb on + strand, within nhaB at 3009.498 kb on + strand, within nhaB at 3009.520 kb on + strand, within nhaB at 3009.530 kb on + strand, within nhaB at 3009.538 kb on - strand at 3009.538 kb on - strand, within nhaB at 3009.544 kb on + strand, within nhaB at 3009.709 kb on + strand, within nhaB at 3009.768 kb on + strand, within nhaB at 3009.845 kb on + strand, within nhaB at 3009.888 kb on - strand, within nhaB at 3009.888 kb on - strand, within nhaB at 3009.938 kb on + strand at 3009.946 kb on - strand at 3009.946 kb on - strand at 3009.991 kb on - strand at 3010.032 kb on + strand at 3010.040 kb on - strand at 3010.053 kb on - strand at 3010.082 kb on - strand at 3010.116 kb on + strand at 3010.194 kb on - strand at 3010.195 kb on + strand at 3010.201 kb on + strand at 3010.209 kb on - strand at 3010.551 kb on - strand, within dsbB at 3010.595 kb on - strand, within dsbB at 3010.626 kb on + strand, within dsbB at 3010.626 kb on + strand, within dsbB at 3010.630 kb on + strand, within dsbB at 3010.663 kb on + strand at 3010.712 kb on + strand at 3010.881 kb on - strand, within SO2888 at 3010.881 kb on - strand, within SO2888 at 3010.920 kb on + strand, within SO2888 at 3010.928 kb on - strand, within SO2888 at 3010.928 kb on - strand, within SO2888 at 3010.937 kb on - strand, within SO2888 at 3010.944 kb on + strand, within SO2888 at 3010.951 kb on + strand, within SO2888 at 3010.971 kb on - strand, within SO2888 at 3010.978 kb on - strand at 3010.985 kb on + strand at 3011.011 kb on + strand at 3011.011 kb on + strand at 3011.011 kb on + strand at 3011.019 kb on - strand at 3011.019 kb on - strand at 3011.019 kb on - strand at 3011.019 kb on - strand at 3011.020 kb on + strand at 3011.021 kb on + strand at 3011.021 kb on + strand at 3011.021 kb on + strand at 3011.021 kb on + strand at 3011.021 kb on + strand at 3011.021 kb on + strand at 3011.029 kb on - strand at 3011.029 kb on - strand at 3011.029 kb on - strand at 3011.029 kb on - strand at 3011.029 kb on - strand at 3011.029 kb on - strand at 3011.029 kb on - strand at 3011.036 kb on + strand at 3011.044 kb on - strand at 3011.099 kb on + strand at 3011.119 kb on + strand at 3011.163 kb on - strand at 3011.179 kb on + strand at 3011.285 kb on - strand at 3011.319 kb on - strand at 3011.391 kb on - strand at 3011.446 kb on - strand at 3011.446 kb on - strand at 3011.451 kb on - strand at 3011.520 kb on + strand at 3011.601 kb on + strand, within SO2889 at 3011.601 kb on + strand, within SO2889 at 3011.601 kb on + strand, within SO2889 at 3011.614 kb on - strand, within SO2889
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,009,240 + nhaB SO2886 0.47 -0.1 3,009,325 - nhaB SO2886 0.53 +1.8 3,009,339 - nhaB SO2886 0.54 +0.3 3,009,410 - nhaB SO2886 0.58 +0.7 3,009,441 + nhaB SO2886 0.60 -0.1 3,009,449 - nhaB SO2886 0.60 +1.4 3,009,454 - nhaB SO2886 0.61 +1.0 3,009,462 + nhaB SO2886 0.61 +3.0 3,009,498 + nhaB SO2886 0.63 +2.2 3,009,520 + nhaB SO2886 0.65 +2.5 3,009,530 + nhaB SO2886 0.65 +1.5 3,009,538 - +1.1 3,009,538 - nhaB SO2886 0.66 +1.8 3,009,544 + nhaB SO2886 0.66 +3.2 3,009,709 + nhaB SO2886 0.77 +1.1 3,009,768 + nhaB SO2886 0.80 +0.5 3,009,845 + nhaB SO2886 0.85 -0.7 3,009,888 - nhaB SO2886 0.88 +0.8 3,009,888 - nhaB SO2886 0.88 +3.7 3,009,938 + +1.3 3,009,946 - +0.2 3,009,946 - +0.3 3,009,991 - +1.3 3,010,032 + +1.3 3,010,040 - +0.2 3,010,053 - +0.6 3,010,082 - -0.7 3,010,116 + +0.1 3,010,194 - +1.3 3,010,195 + +1.0 3,010,201 + +2.9 3,010,209 - +4.1 3,010,551 - dsbB SO2887 0.70 +2.7 3,010,595 - dsbB SO2887 0.79 +3.3 3,010,626 + dsbB SO2887 0.84 -2.6 3,010,626 + dsbB SO2887 0.84 +3.2 3,010,630 + dsbB SO2887 0.85 +3.6 3,010,663 + -1.0 3,010,712 + -0.1 3,010,881 - SO2888 0.56 -0.6 3,010,881 - SO2888 0.56 +0.6 3,010,920 + SO2888 0.70 -0.2 3,010,928 - SO2888 0.73 +1.4 3,010,928 - SO2888 0.73 -1.6 3,010,937 - SO2888 0.76 -0.2 3,010,944 + SO2888 0.79 +4.0 3,010,951 + SO2888 0.81 +2.3 3,010,971 - SO2888 0.89 +1.3 3,010,978 - +0.3 3,010,985 + +0.8 3,011,011 + +1.3 3,011,011 + +4.6 3,011,011 + +2.3 3,011,019 - -1.6 3,011,019 - -1.5 3,011,019 - +3.8 3,011,019 - -0.2 3,011,020 + +2.3 3,011,021 + +0.4 3,011,021 + -0.6 3,011,021 + -0.1 3,011,021 + -0.0 3,011,021 + +1.2 3,011,021 + -2.7 3,011,029 - +0.2 3,011,029 - +1.4 3,011,029 - +1.7 3,011,029 - -0.6 3,011,029 - +1.4 3,011,029 - +2.0 3,011,029 - +0.7 3,011,036 + +0.3 3,011,044 - +0.9 3,011,099 + -2.8 3,011,119 + +2.2 3,011,163 - +0.6 3,011,179 + +1.8 3,011,285 - -1.8 3,011,319 - +1.6 3,011,391 - -1.8 3,011,446 - +1.7 3,011,446 - +1.8 3,011,451 - -2.5 3,011,520 + +1.7 3,011,601 + SO2889 0.11 -0.4 3,011,601 + SO2889 0.11 +2.2 3,011,601 + SO2889 0.11 +0.4 3,011,614 - SO2889 0.11 +1.3
Or see this region's nucleotide sequence