Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2878 and uraA are separated by 215 nucleotides uraA and SO2880 are separated by 132 nucleotides SO2880 and SodB are separated by 226 nucleotides
SO2878: SO2878 - conserved hypothetical protein (NCBI ptt file), at 2,998,243 to 2,999,325
SO2878
SO2879: uraA - uracil permease (NCBI ptt file), at 2,999,541 to 3,000,779
uraA
SO2880: SO2880 - glutaredoxin domain protein (NCBI ptt file), at 3,000,912 to 3,001,244
SO2880
SO2881: SodB - superoxide dismutase, Fe (NCBI ptt file), at 3,001,471 to 3,002,055
SodB
Position (kb)
2999
3000
3001 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2999.281 kb on + strand at 2999.378 kb on - strand at 2999.388 kb on + strand at 2999.388 kb on + strand at 2999.411 kb on + strand at 2999.411 kb on + strand at 2999.411 kb on + strand at 2999.419 kb on - strand at 2999.436 kb on + strand at 2999.444 kb on - strand at 2999.567 kb on - strand at 2999.648 kb on + strand at 2999.667 kb on + strand, within uraA at 2999.907 kb on + strand, within uraA at 2999.921 kb on - strand, within uraA at 2999.945 kb on + strand, within uraA at 2999.953 kb on - strand, within uraA at 2999.973 kb on - strand, within uraA at 2999.973 kb on - strand, within uraA at 2999.984 kb on - strand, within uraA at 2999.984 kb on - strand, within uraA at 3000.056 kb on + strand, within uraA at 3000.227 kb on + strand, within uraA at 3000.235 kb on - strand, within uraA at 3000.462 kb on + strand, within uraA at 3000.564 kb on + strand, within uraA at 3000.633 kb on + strand, within uraA at 3000.639 kb on + strand, within uraA at 3000.641 kb on - strand, within uraA at 3000.676 kb on + strand at 3000.699 kb on - strand at 3000.716 kb on - strand at 3000.809 kb on - strand at 3000.861 kb on + strand at 3000.861 kb on + strand at 3000.932 kb on + strand at 3001.188 kb on - strand, within SO2880 at 3001.228 kb on - strand at 3001.295 kb on + strand at 3001.329 kb on + strand at 3001.329 kb on + strand at 3001.417 kb on - strand at 3001.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,999,281 + -1.5 2,999,378 - +0.9 2,999,388 + +0.1 2,999,388 + +2.7 2,999,411 + +0.8 2,999,411 + -0.4 2,999,411 + -1.0 2,999,419 - +1.1 2,999,436 + +0.3 2,999,444 - +0.8 2,999,567 - +3.1 2,999,648 + +1.9 2,999,667 + uraA SO2879 0.10 +5.0 2,999,907 + uraA SO2879 0.30 +3.1 2,999,921 - uraA SO2879 0.31 +3.5 2,999,945 + uraA SO2879 0.33 +1.8 2,999,953 - uraA SO2879 0.33 +2.5 2,999,973 - uraA SO2879 0.35 +5.7 2,999,973 - uraA SO2879 0.35 -0.2 2,999,984 - uraA SO2879 0.36 +1.4 2,999,984 - uraA SO2879 0.36 +3.8 3,000,056 + uraA SO2879 0.42 -1.2 3,000,227 + uraA SO2879 0.55 +3.7 3,000,235 - uraA SO2879 0.56 +1.4 3,000,462 + uraA SO2879 0.74 +4.2 3,000,564 + uraA SO2879 0.83 +0.4 3,000,633 + uraA SO2879 0.88 +2.4 3,000,639 + uraA SO2879 0.89 +1.9 3,000,641 - uraA SO2879 0.89 -3.4 3,000,676 + +2.5 3,000,699 - +3.1 3,000,716 - +1.9 3,000,809 - -2.2 3,000,861 + -2.2 3,000,861 + +1.4 3,000,932 + -2.8 3,001,188 - SO2880 0.83 -3.7 3,001,228 - -1.2 3,001,295 + -3.8 3,001,329 + -0.3 3,001,329 + +0.9 3,001,417 - -0.2 3,001,417 - -1.7
Or see this region's nucleotide sequence