Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2874 and SO2875 overlap by 11 nucleotides SO2875 and SO2876 are separated by 62 nucleotides SO2876 and SO2877 are separated by 114 nucleotides SO2877 and SO2878 are separated by 75 nucleotides
SO2874: SO2874 - ISSod10, transposase OrfA (NCBI ptt file), at 2,994,301 to 2,994,780
SO2874
SO2875: SO2875 - ISSod10, transposase OrfB (NCBI ptt file), at 2,994,770 to 2,995,327
SO2875
SO2876: SO2876 - hypothetical protein (NCBI ptt file), at 2,995,390 to 2,997,342
SO2876
SO2877: SO2877 - conserved hypothetical protein (NCBI ptt file), at 2,997,457 to 2,998,167
SO2877
SO2878: SO2878 - conserved hypothetical protein (NCBI ptt file), at 2,998,243 to 2,999,325
SO2878
Position (kb)
2995
2996
2997
2998 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2995.383 kb on + strand at 2995.447 kb on - strand at 2995.595 kb on + strand, within SO2876 at 2995.603 kb on - strand, within SO2876 at 2995.714 kb on + strand, within SO2876 at 2995.714 kb on + strand, within SO2876 at 2996.065 kb on + strand, within SO2876 at 2996.065 kb on + strand, within SO2876 at 2996.065 kb on + strand, within SO2876 at 2996.073 kb on - strand, within SO2876 at 2996.127 kb on - strand, within SO2876 at 2996.161 kb on + strand, within SO2876 at 2996.202 kb on + strand, within SO2876 at 2996.346 kb on + strand, within SO2876 at 2996.392 kb on - strand, within SO2876 at 2996.474 kb on + strand, within SO2876 at 2996.474 kb on + strand, within SO2876 at 2996.482 kb on - strand, within SO2876 at 2996.615 kb on + strand, within SO2876 at 2996.653 kb on + strand, within SO2876 at 2996.653 kb on + strand, within SO2876 at 2996.727 kb on - strand, within SO2876 at 2996.787 kb on - strand, within SO2876 at 2996.807 kb on + strand, within SO2876 at 2996.810 kb on - strand, within SO2876 at 2996.960 kb on - strand, within SO2876 at 2997.123 kb on - strand, within SO2876 at 2997.159 kb on - strand at 2997.160 kb on - strand at 2997.261 kb on - strand at 2997.293 kb on + strand at 2997.293 kb on + strand at 2997.455 kb on + strand at 2997.455 kb on + strand at 2997.801 kb on + strand, within SO2877 at 2997.979 kb on + strand, within SO2877 at 2997.987 kb on - strand, within SO2877 at 2998.041 kb on + strand, within SO2877 at 2998.156 kb on + strand at 2998.164 kb on - strand at 2998.292 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,995,383 + -2.3 2,995,447 - +1.2 2,995,595 + SO2876 0.10 -0.5 2,995,603 - SO2876 0.11 +3.0 2,995,714 + SO2876 0.17 +0.3 2,995,714 + SO2876 0.17 +1.1 2,996,065 + SO2876 0.35 +0.3 2,996,065 + SO2876 0.35 +0.8 2,996,065 + SO2876 0.35 +1.1 2,996,073 - SO2876 0.35 -0.0 2,996,127 - SO2876 0.38 +0.6 2,996,161 + SO2876 0.39 +2.7 2,996,202 + SO2876 0.42 +2.0 2,996,346 + SO2876 0.49 +1.6 2,996,392 - SO2876 0.51 +0.6 2,996,474 + SO2876 0.56 -1.4 2,996,474 + SO2876 0.56 -2.8 2,996,482 - SO2876 0.56 +2.4 2,996,615 + SO2876 0.63 +0.6 2,996,653 + SO2876 0.65 +0.2 2,996,653 + SO2876 0.65 +0.6 2,996,727 - SO2876 0.68 +0.4 2,996,787 - SO2876 0.72 +1.8 2,996,807 + SO2876 0.73 -1.9 2,996,810 - SO2876 0.73 -0.3 2,996,960 - SO2876 0.80 -1.7 2,997,123 - SO2876 0.89 +0.7 2,997,159 - -2.4 2,997,160 - -1.2 2,997,261 - +0.3 2,997,293 + +2.0 2,997,293 + -0.5 2,997,455 + -1.1 2,997,455 + -1.2 2,997,801 + SO2877 0.48 -2.8 2,997,979 + SO2877 0.73 +0.4 2,997,987 - SO2877 0.75 -3.0 2,998,041 + SO2877 0.82 +0.1 2,998,156 + -0.1 2,998,164 - -0.4 2,998,292 + -2.0
Or see this region's nucleotide sequence