Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2757 and SO2758 are separated by 76 nucleotides SO2758 and upp are separated by 189 nucleotides
SO2757: SO2757 - membrane protein, putative (NCBI ptt file), at 2,878,837 to 2,880,156
SO2757
SO2758: SO2758 - GGDEF family protein (NCBI ptt file), at 2,880,233 to 2,881,636
SO2758
SO2759: upp - uracil phosphoribosyltransferase (NCBI ptt file), at 2,881,826 to 2,882,452
upp
Position (kb)
2880
2881
2882 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2879.279 kb on + strand, within SO2757 at 2879.279 kb on + strand, within SO2757 at 2879.291 kb on + strand, within SO2757 at 2879.291 kb on + strand, within SO2757 at 2879.498 kb on - strand, within SO2757 at 2879.595 kb on + strand, within SO2757 at 2879.624 kb on - strand, within SO2757 at 2879.677 kb on + strand, within SO2757 at 2879.781 kb on - strand, within SO2757 at 2879.940 kb on - strand, within SO2757 at 2879.947 kb on - strand, within SO2757 at 2880.124 kb on - strand at 2880.303 kb on + strand at 2880.303 kb on + strand at 2880.311 kb on - strand at 2880.351 kb on + strand at 2880.359 kb on - strand at 2880.388 kb on + strand, within SO2758 at 2880.401 kb on - strand, within SO2758 at 2880.556 kb on - strand, within SO2758 at 2880.561 kb on - strand, within SO2758 at 2880.600 kb on + strand, within SO2758 at 2880.700 kb on - strand, within SO2758 at 2880.769 kb on + strand, within SO2758 at 2881.036 kb on + strand, within SO2758 at 2881.335 kb on + strand, within SO2758 at 2881.402 kb on + strand, within SO2758 at 2881.598 kb on + strand at 2881.606 kb on - strand at 2881.859 kb on + strand at 2881.867 kb on - strand at 2881.867 kb on - strand at 2881.873 kb on + strand at 2881.873 kb on + strand at 2881.873 kb on + strand at 2881.873 kb on + strand at 2881.881 kb on - strand at 2881.881 kb on - strand at 2881.881 kb on - strand at 2881.881 kb on - strand at 2881.914 kb on + strand, within upp at 2881.914 kb on + strand, within upp at 2881.914 kb on + strand, within upp at 2881.969 kb on - strand, within upp at 2882.125 kb on + strand, within upp at 2882.133 kb on - strand, within upp at 2882.133 kb on - strand, within upp at 2882.133 kb on - strand, within upp at 2882.133 kb on - strand, within upp at 2882.207 kb on + strand, within upp at 2882.215 kb on - strand, within upp at 2882.250 kb on + strand, within upp at 2882.392 kb on - strand at 2882.439 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,879,279 + SO2757 0.33 +1.2 2,879,279 + SO2757 0.33 +0.4 2,879,291 + SO2757 0.34 -1.2 2,879,291 + SO2757 0.34 -0.1 2,879,498 - SO2757 0.50 +1.1 2,879,595 + SO2757 0.57 -0.3 2,879,624 - SO2757 0.60 -2.6 2,879,677 + SO2757 0.64 -0.5 2,879,781 - SO2757 0.72 -0.8 2,879,940 - SO2757 0.84 -2.6 2,879,947 - SO2757 0.84 +0.3 2,880,124 - +0.4 2,880,303 + +0.1 2,880,303 + +1.0 2,880,311 - +0.2 2,880,351 + -2.0 2,880,359 - -2.8 2,880,388 + SO2758 0.11 +0.6 2,880,401 - SO2758 0.12 +0.4 2,880,556 - SO2758 0.23 -2.1 2,880,561 - SO2758 0.23 -3.0 2,880,600 + SO2758 0.26 +0.2 2,880,700 - SO2758 0.33 +1.3 2,880,769 + SO2758 0.38 -1.4 2,881,036 + SO2758 0.57 -1.7 2,881,335 + SO2758 0.78 -3.0 2,881,402 + SO2758 0.83 -1.0 2,881,598 + +0.5 2,881,606 - +0.2 2,881,859 + +2.5 2,881,867 - +4.2 2,881,867 - +1.3 2,881,873 + +1.6 2,881,873 + -0.8 2,881,873 + -2.0 2,881,873 + -1.5 2,881,881 - -0.5 2,881,881 - +2.3 2,881,881 - -3.0 2,881,881 - -4.3 2,881,914 + upp SO2759 0.14 -0.6 2,881,914 + upp SO2759 0.14 +1.3 2,881,914 + upp SO2759 0.14 +1.4 2,881,969 - upp SO2759 0.23 +1.0 2,882,125 + upp SO2759 0.48 +0.7 2,882,133 - upp SO2759 0.49 -1.8 2,882,133 - upp SO2759 0.49 +2.9 2,882,133 - upp SO2759 0.49 +0.8 2,882,133 - upp SO2759 0.49 -0.1 2,882,207 + upp SO2759 0.61 +4.3 2,882,215 - upp SO2759 0.62 +1.2 2,882,250 + upp SO2759 0.68 +2.9 2,882,392 - +3.9 2,882,439 - -1.7
Or see this region's nucleotide sequence