Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2660 and SO2661 are separated by 225 nucleotides SO2661 and SO2662 overlap by 20 nucleotides SO2662 and SO2662.1 overlap by 234 nucleotides SO2662.1 and SO2663 are separated by 94 nucleotides SO2663 and SO2664 are separated by 83 nucleotides SO2664 and SO2665 are separated by 35 nucleotides SO2665 and SO2666 are separated by 9 nucleotides
SO2660: SO2660 - conserved hypothetical protein (NCBI ptt file), at 2,793,600 to 2,794,220
SO2660
SO2661: SO2661 - hypothetical protein (NCBI ptt file), at 2,794,446 to 2,794,721
SO2661
SO2662: SO2662 - hypothetical protein (NCBI ptt file), at 2,794,702 to 2,794,938
SO2662
SO2662.1: SO2662.1 - hypothetical protein (NCBI ptt file), at 2,794,705 to 2,794,887
SO2662.1
SO2663: SO2663 - conserved hypothetical protein (NCBI ptt file), at 2,794,982 to 2,795,551
SO2663
SO2664: SO2664 - hypothetical protein (NCBI ptt file), at 2,795,635 to 2,795,928
SO2664
SO2665: SO2665 - hypothetical protein (NCBI ptt file), at 2,795,964 to 2,796,221
SO2665
SO2666: SO2666 - hypothetical protein (NCBI ptt file), at 2,796,231 to 2,796,797
SO2666
Position (kb)
2794
2795
2796 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2794.050 kb on - strand, within SO2660 at 2794.050 kb on - strand, within SO2660 at 2794.183 kb on + strand at 2794.250 kb on + strand at 2794.295 kb on + strand at 2794.303 kb on - strand at 2794.531 kb on - strand, within SO2661 at 2794.634 kb on - strand, within SO2661 at 2794.643 kb on + strand, within SO2661 at 2794.643 kb on + strand, within SO2661 at 2794.651 kb on - strand, within SO2661 at 2794.671 kb on - strand, within SO2661 at 2794.702 kb on - strand at 2794.771 kb on - strand at 2794.989 kb on + strand at 2795.013 kb on - strand at 2795.018 kb on - strand at 2795.077 kb on + strand, within SO2663 at 2795.111 kb on + strand, within SO2663 at 2795.185 kb on + strand, within SO2663 at 2795.192 kb on - strand, within SO2663 at 2795.221 kb on - strand, within SO2663 at 2795.276 kb on - strand, within SO2663 at 2795.331 kb on - strand, within SO2663 at 2795.333 kb on - strand, within SO2663 at 2795.364 kb on - strand, within SO2663 at 2795.364 kb on - strand, within SO2663 at 2795.366 kb on + strand, within SO2663 at 2795.374 kb on - strand, within SO2663 at 2795.398 kb on + strand, within SO2663 at 2795.424 kb on + strand, within SO2663 at 2795.432 kb on - strand, within SO2663 at 2795.432 kb on - strand, within SO2663 at 2795.432 kb on - strand, within SO2663 at 2795.434 kb on + strand, within SO2663 at 2795.434 kb on + strand, within SO2663 at 2795.434 kb on + strand, within SO2663 at 2795.449 kb on - strand, within SO2663 at 2795.531 kb on + strand at 2795.534 kb on - strand at 2795.539 kb on - strand at 2795.574 kb on - strand at 2795.654 kb on + strand at 2795.662 kb on - strand at 2795.662 kb on - strand at 2795.706 kb on - strand, within SO2664 at 2795.783 kb on + strand, within SO2664 at 2795.783 kb on + strand, within SO2664 at 2795.783 kb on + strand, within SO2664 at 2795.783 kb on + strand, within SO2664 at 2795.783 kb on + strand, within SO2664 at 2795.783 kb on + strand, within SO2664 at 2795.783 kb on + strand, within SO2664 at 2795.791 kb on - strand, within SO2664 at 2795.791 kb on - strand, within SO2664 at 2795.791 kb on - strand, within SO2664 at 2795.799 kb on + strand, within SO2664 at 2795.812 kb on + strand, within SO2664 at 2795.992 kb on + strand, within SO2665 at 2796.054 kb on - strand, within SO2665 at 2796.054 kb on - strand, within SO2665 at 2796.054 kb on - strand, within SO2665 at 2796.104 kb on + strand, within SO2665 at 2796.112 kb on - strand, within SO2665 at 2796.197 kb on - strand at 2796.216 kb on + strand at 2796.216 kb on + strand at 2796.216 kb on + strand at 2796.279 kb on - strand at 2796.282 kb on - strand at 2796.336 kb on - strand, within SO2666 at 2796.346 kb on - strand, within SO2666 at 2796.351 kb on - strand, within SO2666 at 2796.375 kb on + strand, within SO2666 at 2796.442 kb on - strand, within SO2666 at 2796.442 kb on - strand, within SO2666 at 2796.442 kb on - strand, within SO2666 at 2796.467 kb on + strand, within SO2666
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,794,050 - SO2660 0.72 -0.3 2,794,050 - SO2660 0.72 +0.0 2,794,183 + -4.6 2,794,250 + -1.8 2,794,295 + -4.0 2,794,303 - -0.5 2,794,531 - SO2661 0.31 -2.9 2,794,634 - SO2661 0.68 -0.4 2,794,643 + SO2661 0.71 -2.1 2,794,643 + SO2661 0.71 -2.5 2,794,651 - SO2661 0.74 -1.5 2,794,671 - SO2661 0.82 -1.6 2,794,702 - -0.6 2,794,771 - -2.1 2,794,989 + -1.3 2,795,013 - +0.3 2,795,018 - +0.7 2,795,077 + SO2663 0.17 -2.5 2,795,111 + SO2663 0.23 -1.8 2,795,185 + SO2663 0.36 -1.2 2,795,192 - SO2663 0.37 -1.5 2,795,221 - SO2663 0.42 -1.6 2,795,276 - SO2663 0.52 -0.4 2,795,331 - SO2663 0.61 +0.7 2,795,333 - SO2663 0.62 +1.9 2,795,364 - SO2663 0.67 -0.7 2,795,364 - SO2663 0.67 -2.2 2,795,366 + SO2663 0.67 -1.5 2,795,374 - SO2663 0.69 -1.3 2,795,398 + SO2663 0.73 -3.7 2,795,424 + SO2663 0.78 -4.6 2,795,432 - SO2663 0.79 -1.7 2,795,432 - SO2663 0.79 -1.9 2,795,432 - SO2663 0.79 -3.5 2,795,434 + SO2663 0.79 -1.5 2,795,434 + SO2663 0.79 -0.6 2,795,434 + SO2663 0.79 -1.1 2,795,449 - SO2663 0.82 -0.6 2,795,531 + -4.1 2,795,534 - -0.8 2,795,539 - +1.1 2,795,574 - -1.3 2,795,654 + -2.9 2,795,662 - -0.5 2,795,662 - -1.1 2,795,706 - SO2664 0.24 -3.9 2,795,783 + SO2664 0.50 -4.7 2,795,783 + SO2664 0.50 -0.7 2,795,783 + SO2664 0.50 -0.7 2,795,783 + SO2664 0.50 -0.8 2,795,783 + SO2664 0.50 +0.7 2,795,783 + SO2664 0.50 -3.4 2,795,783 + SO2664 0.50 -1.5 2,795,791 - SO2664 0.53 -0.5 2,795,791 - SO2664 0.53 +0.6 2,795,791 - SO2664 0.53 -0.4 2,795,799 + SO2664 0.56 -4.6 2,795,812 + SO2664 0.60 -2.5 2,795,992 + SO2665 0.11 +1.6 2,796,054 - SO2665 0.35 -3.5 2,796,054 - SO2665 0.35 -0.1 2,796,054 - SO2665 0.35 +0.8 2,796,104 + SO2665 0.54 -1.5 2,796,112 - SO2665 0.57 +0.8 2,796,197 - +0.9 2,796,216 + -0.6 2,796,216 + +1.1 2,796,216 + +0.0 2,796,279 - +0.5 2,796,282 - -0.6 2,796,336 - SO2666 0.19 +0.1 2,796,346 - SO2666 0.20 -0.7 2,796,351 - SO2666 0.21 -0.3 2,796,375 + SO2666 0.25 -0.5 2,796,442 - SO2666 0.37 +1.7 2,796,442 - SO2666 0.37 -1.1 2,796,442 - SO2666 0.37 -1.4 2,796,467 + SO2666 0.42 +0.8
Or see this region's nucleotide sequence