Strain Fitness in Shewanella oneidensis MR-1 around SO2645

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntppsA and SO2645 are separated by 125 nucleotidesSO2645 and aroH are separated by 182 nucleotides SO2644: ppsA - phosphoenolpyruvate synthase (NCBI ptt file), at 2,779,864 to 2,782,233 ppsA SO2645: SO2645 - conserved hypothetical protein (NCBI ptt file), at 2,782,359 to 2,783,171 SO2645 SO2646: aroH - phospho-2-dehydro-3-deoxyheptonate aldolase, trp-sensitive (NCBI ptt file), at 2,783,354 to 2,784,418 aroH Position (kb) 2782 2783 2784Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2781.560 kb on + strand, within ppsAat 2781.709 kb on + strand, within ppsAat 2781.812 kb on + strand, within ppsAat 2781.863 kb on + strand, within ppsAat 2781.952 kb on - strand, within ppsAat 2781.952 kb on - strand, within ppsAat 2781.997 kb on - strandat 2782.225 kb on + strandat 2782.402 kb on + strandat 2782.418 kb on - strandat 2782.652 kb on + strand, within SO2645at 2782.677 kb on + strand, within SO2645at 2782.677 kb on + strand, within SO2645at 2782.677 kb on + strand, within SO2645at 2782.677 kb on + strand, within SO2645at 2782.677 kb on + strand, within SO2645at 2782.685 kb on - strand, within SO2645at 2782.685 kb on - strand, within SO2645at 2782.685 kb on - strand, within SO2645at 2782.685 kb on - strand, within SO2645at 2782.685 kb on - strand, within SO2645at 2782.748 kb on + strand, within SO2645at 2782.748 kb on + strand, within SO2645at 2782.756 kb on - strand, within SO2645at 2782.783 kb on - strand, within SO2645at 2782.792 kb on + strand, within SO2645at 2782.792 kb on + strand, within SO2645at 2782.845 kb on - strand, within SO2645at 2782.906 kb on + strand, within SO2645at 2782.906 kb on + strand, within SO2645at 2782.906 kb on + strand, within SO2645at 2782.906 kb on + strand, within SO2645at 2782.906 kb on + strand, within SO2645at 2782.906 kb on + strand, within SO2645at 2782.914 kb on - strand, within SO2645at 2782.914 kb on - strand, within SO2645at 2782.914 kb on - strand, within SO2645at 2782.914 kb on - strand, within SO2645at 2782.914 kb on - strand, within SO2645at 2783.012 kb on - strand, within SO2645at 2783.036 kb on - strand, within SO2645at 2783.157 kb on - strandat 2783.181 kb on + strandat 2783.189 kb on - strandat 2783.189 kb on - strandat 2783.191 kb on + strandat 2783.280 kb on + strandat 2783.280 kb on + strandat 2783.280 kb on + strandat 2783.280 kb on + strandat 2783.288 kb on - strandat 2783.288 kb on - strandat 2783.288 kb on - strandat 2783.367 kb on - strandat 2783.367 kb on - strandat 2783.409 kb on - strandat 2783.480 kb on + strand, within aroHat 2783.583 kb on - strand, within aroHat 2783.758 kb on + strand, within aroHat 2783.859 kb on + strand, within aroHat 2783.867 kb on - strand, within aroHat 2783.867 kb on - strand, within aroHat 2783.887 kb on - strand, within aroHat 2784.131 kb on - strand, within aroH

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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2,781,560 + ppsA SO2644 0.72 -2.7
2,781,709 + ppsA SO2644 0.78 -3.7
2,781,812 + ppsA SO2644 0.82 -4.2
2,781,863 + ppsA SO2644 0.84 -3.5
2,781,952 - ppsA SO2644 0.88 -1.9
2,781,952 - ppsA SO2644 0.88 -1.9
2,781,997 - -4.1
2,782,225 + -1.5
2,782,402 + -1.5
2,782,418 - -2.2
2,782,652 + SO2645 0.36 -4.4
2,782,677 + SO2645 0.39 -1.1
2,782,677 + SO2645 0.39 -1.5
2,782,677 + SO2645 0.39 -0.9
2,782,677 + SO2645 0.39 -0.8
2,782,677 + SO2645 0.39 -4.2
2,782,685 - SO2645 0.40 -2.9
2,782,685 - SO2645 0.40 -2.0
2,782,685 - SO2645 0.40 +0.4
2,782,685 - SO2645 0.40 -2.7
2,782,685 - SO2645 0.40 -0.9
2,782,748 + SO2645 0.48 -0.8
2,782,748 + SO2645 0.48 -1.4
2,782,756 - SO2645 0.49 -1.2
2,782,783 - SO2645 0.52 -1.5
2,782,792 + SO2645 0.53 +1.0
2,782,792 + SO2645 0.53 -0.8
2,782,845 - SO2645 0.60 -3.6
2,782,906 + SO2645 0.67 -2.5
2,782,906 + SO2645 0.67 +1.1
2,782,906 + SO2645 0.67 +0.7
2,782,906 + SO2645 0.67 -1.2
2,782,906 + SO2645 0.67 -0.5
2,782,906 + SO2645 0.67 -0.3
2,782,914 - SO2645 0.68 -0.8
2,782,914 - SO2645 0.68 -4.4
2,782,914 - SO2645 0.68 -1.4
2,782,914 - SO2645 0.68 -2.2
2,782,914 - SO2645 0.68 -2.7
2,783,012 - SO2645 0.80 -1.5
2,783,036 - SO2645 0.83 -2.7
2,783,157 - +2.4
2,783,181 + -2.4
2,783,189 - +1.7
2,783,189 - -0.0
2,783,191 + -1.7
2,783,280 + +0.1
2,783,280 + -3.1
2,783,280 + -0.5
2,783,280 + +0.2
2,783,288 - -0.4
2,783,288 - +1.2
2,783,288 - -0.3
2,783,367 - -3.7
2,783,367 - +0.6
2,783,409 - -1.2
2,783,480 + aroH SO2646 0.12 -2.2
2,783,583 - aroH SO2646 0.22 -1.9
2,783,758 + aroH SO2646 0.38 +0.3
2,783,859 + aroH SO2646 0.47 +0.1
2,783,867 - aroH SO2646 0.48 -2.5
2,783,867 - aroH SO2646 0.48 -0.9
2,783,887 - aroH SO2646 0.50 +0.4
2,784,131 - aroH SO2646 0.73 -0.0

Or see this region's nucleotide sequence