Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2582 and SO2583 are separated by 21 nucleotides SO2583 and SO2584 are separated by 144 nucleotides SO2584 and SO2585 overlap by 20 nucleotides
SO2582: SO2582 - hydrolase, alpha/beta fold family (NCBI ptt file), at 2,709,757 to 2,710,623
SO2582
SO2583: SO2583 - hypothetical protein (NCBI ptt file), at 2,710,645 to 2,711,457
SO2583
SO2584: SO2584 - hypothetical protein (NCBI ptt file), at 2,711,602 to 2,712,315
SO2584
SO2585: SO2585 - hypothetical protein (NCBI ptt file), at 2,712,296 to 2,712,604
SO2585
Position (kb)
2710
2711
2712 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2709.656 kb on - strand at 2709.866 kb on + strand, within SO2582 at 2709.874 kb on - strand, within SO2582 at 2709.874 kb on - strand, within SO2582 at 2709.874 kb on - strand, within SO2582 at 2709.960 kb on + strand, within SO2582 at 2709.967 kb on + strand, within SO2582 at 2709.968 kb on - strand, within SO2582 at 2709.968 kb on - strand, within SO2582 at 2710.107 kb on - strand, within SO2582 at 2710.138 kb on - strand, within SO2582 at 2710.138 kb on - strand, within SO2582 at 2710.214 kb on + strand, within SO2582 at 2710.236 kb on - strand, within SO2582 at 2710.723 kb on + strand at 2710.763 kb on + strand, within SO2583 at 2710.765 kb on - strand, within SO2583 at 2710.765 kb on - strand, within SO2583 at 2710.771 kb on - strand, within SO2583 at 2710.900 kb on + strand, within SO2583 at 2710.938 kb on + strand, within SO2583 at 2710.938 kb on + strand, within SO2583 at 2711.165 kb on + strand, within SO2583 at 2711.173 kb on - strand, within SO2583 at 2711.205 kb on - strand, within SO2583 at 2711.324 kb on - strand, within SO2583 at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.406 kb on - strand at 2711.406 kb on - strand at 2711.481 kb on + strand at 2711.483 kb on - strand at 2711.536 kb on + strand at 2711.593 kb on + strand at 2711.730 kb on - strand, within SO2584 at 2711.855 kb on - strand, within SO2584 at 2712.175 kb on + strand, within SO2584 at 2712.183 kb on - strand, within SO2584 at 2712.285 kb on - strand at 2712.408 kb on + strand, within SO2585 at 2712.416 kb on - strand, within SO2585
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,709,656 - +4.9 2,709,866 + SO2582 0.13 +0.4 2,709,874 - SO2582 0.13 +1.2 2,709,874 - SO2582 0.13 +1.3 2,709,874 - SO2582 0.13 -0.2 2,709,960 + SO2582 0.23 +0.4 2,709,967 + SO2582 0.24 +2.1 2,709,968 - SO2582 0.24 -0.3 2,709,968 - SO2582 0.24 +0.2 2,710,107 - SO2582 0.40 -0.6 2,710,138 - SO2582 0.44 +0.5 2,710,138 - SO2582 0.44 -1.9 2,710,214 + SO2582 0.53 -0.1 2,710,236 - SO2582 0.55 -1.1 2,710,723 + -0.3 2,710,763 + SO2583 0.15 -0.7 2,710,765 - SO2583 0.15 -1.2 2,710,765 - SO2583 0.15 -0.1 2,710,771 - SO2583 0.15 -0.0 2,710,900 + SO2583 0.31 -0.7 2,710,938 + SO2583 0.36 +0.6 2,710,938 + SO2583 0.36 +0.5 2,711,165 + SO2583 0.64 -1.2 2,711,173 - SO2583 0.65 -0.6 2,711,205 - SO2583 0.69 -1.9 2,711,324 - SO2583 0.84 -0.5 2,711,398 + +1.9 2,711,398 + -1.3 2,711,398 + +1.0 2,711,398 + -2.7 2,711,398 + +1.3 2,711,398 + -1.5 2,711,398 + -0.2 2,711,398 + -0.1 2,711,406 - -0.9 2,711,406 - -0.1 2,711,481 + -0.4 2,711,483 - -0.4 2,711,536 + +1.1 2,711,593 + -0.6 2,711,730 - SO2584 0.18 -0.5 2,711,855 - SO2584 0.35 -1.9 2,712,175 + SO2584 0.80 -1.7 2,712,183 - SO2584 0.81 -0.8 2,712,285 - +0.4 2,712,408 + SO2585 0.36 -2.3 2,712,416 - SO2585 0.39 +0.7
Or see this region's nucleotide sequence