Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt minD and minE are separated by 2 nucleotides minE and rnd are separated by 238 nucleotides rnd and fadD-1 are separated by 76 nucleotides
SO2577: minD - septum site-determining protein MinD (NCBI ptt file), at 2,705,398 to 2,706,207
minD
SO2578: minE - cell division topological specificity factor MinE (NCBI ptt file), at 2,706,210 to 2,706,467
minE
SO2580: rnd - ribonuclease D (NCBI ptt file), at 2,706,706 to 2,707,809
rnd
SO2581: fadD-1 - long-chain-fatty-acid--CoA ligase (NCBI ptt file), at 2,707,886 to 2,709,559
fadD-1
Position (kb)
2706
2707
2708 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2705.825 kb on + strand, within minD at 2705.880 kb on - strand, within minD at 2705.903 kb on + strand, within minD at 2706.119 kb on - strand, within minD at 2706.135 kb on + strand at 2706.607 kb on - strand at 2706.980 kb on - strand, within rnd at 2707.060 kb on - strand, within rnd at 2707.105 kb on + strand, within rnd at 2707.113 kb on - strand, within rnd at 2707.152 kb on - strand, within rnd at 2707.158 kb on + strand, within rnd at 2707.441 kb on + strand, within rnd at 2707.462 kb on + strand, within rnd at 2707.470 kb on - strand, within rnd at 2707.470 kb on - strand, within rnd at 2707.934 kb on - strand at 2707.951 kb on + strand at 2708.357 kb on - strand, within fadD-1 at 2708.468 kb on + strand, within fadD-1 at 2708.715 kb on - strand, within fadD-1 at 2708.715 kb on - strand, within fadD-1
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,705,825 + minD SO2577 0.53 +0.5 2,705,880 - minD SO2577 0.60 +2.4 2,705,903 + minD SO2577 0.62 +1.8 2,706,119 - minD SO2577 0.89 +0.2 2,706,135 + -0.3 2,706,607 - +1.3 2,706,980 - rnd SO2580 0.25 +0.4 2,707,060 - rnd SO2580 0.32 -1.9 2,707,105 + rnd SO2580 0.36 +0.2 2,707,113 - rnd SO2580 0.37 -0.0 2,707,152 - rnd SO2580 0.40 +0.3 2,707,158 + rnd SO2580 0.41 -0.8 2,707,441 + rnd SO2580 0.67 -0.6 2,707,462 + rnd SO2580 0.68 +1.7 2,707,470 - rnd SO2580 0.69 -0.5 2,707,470 - rnd SO2580 0.69 -1.1 2,707,934 - -1.4 2,707,951 + -0.1 2,708,357 - fadD-1 SO2581 0.28 -0.9 2,708,468 + fadD-1 SO2581 0.35 -2.7 2,708,715 - fadD-1 SO2581 0.50 +0.2 2,708,715 - fadD-1 SO2581 0.50 -1.3
Or see this region's nucleotide sequence