Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rnhA and SO2561 are separated by 28 nucleotides SO2561 and SO2562 are separated by 23 nucleotides SO2562 and SO2563 are separated by 160 nucleotides
SO2560: rnhA - ribonuclease HI (NCBI ptt file), at 2,690,175 to 2,690,651
rnhA
SO2561: SO2561 - transcriptional regulator, LysR family (NCBI ptt file), at 2,690,680 to 2,691,579
SO2561
SO2562: SO2562 - conserved hypothetical protein (NCBI ptt file), at 2,691,603 to 2,692,337
SO2562
SO2563: SO2563 - metallo-beta-lactamase family protein (NCBI ptt file), at 2,692,498 to 2,693,301
SO2563
Position (kb)
2691
2692
2693 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2690.730 kb on + strand at 2690.802 kb on - strand, within SO2561 at 2690.812 kb on + strand, within SO2561 at 2690.820 kb on - strand, within SO2561 at 2691.002 kb on + strand, within SO2561 at 2691.010 kb on - strand, within SO2561 at 2691.130 kb on - strand, within SO2561 at 2691.389 kb on - strand, within SO2561 at 2691.485 kb on + strand, within SO2561 at 2691.512 kb on + strand at 2691.528 kb on - strand at 2691.530 kb on - strand at 2691.641 kb on - strand at 2691.761 kb on + strand, within SO2562 at 2691.769 kb on - strand, within SO2562 at 2691.800 kb on - strand, within SO2562 at 2691.850 kb on - strand, within SO2562 at 2691.852 kb on + strand, within SO2562 at 2691.857 kb on + strand, within SO2562 at 2691.857 kb on + strand, within SO2562 at 2691.860 kb on - strand, within SO2562 at 2691.909 kb on - strand, within SO2562 at 2691.909 kb on - strand, within SO2562 at 2691.954 kb on + strand, within SO2562 at 2692.312 kb on - strand at 2692.319 kb on + strand at 2692.321 kb on + strand at 2692.329 kb on - strand at 2692.408 kb on - strand at 2692.501 kb on - strand at 2692.579 kb on - strand, within SO2563 at 2692.662 kb on + strand, within SO2563 at 2692.664 kb on + strand, within SO2563 at 2692.665 kb on + strand, within SO2563 at 2692.723 kb on + strand, within SO2563 at 2692.837 kb on - strand, within SO2563 at 2692.853 kb on - strand, within SO2563 at 2692.965 kb on - strand, within SO2563 at 2692.981 kb on - strand, within SO2563 at 2693.010 kb on + strand, within SO2563 at 2693.082 kb on + strand, within SO2563 at 2693.198 kb on + strand, within SO2563 at 2693.210 kb on + strand, within SO2563 at 2693.210 kb on + strand, within SO2563 at 2693.210 kb on + strand, within SO2563 at 2693.210 kb on + strand, within SO2563 at 2693.246 kb on + strand at 2693.273 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,690,730 + +1.7 2,690,802 - SO2561 0.14 +1.2 2,690,812 + SO2561 0.15 +1.0 2,690,820 - SO2561 0.16 +1.8 2,691,002 + SO2561 0.36 +0.3 2,691,010 - SO2561 0.37 +1.2 2,691,130 - SO2561 0.50 +1.0 2,691,389 - SO2561 0.79 +0.6 2,691,485 + SO2561 0.89 +0.2 2,691,512 + +1.1 2,691,528 - -2.7 2,691,530 - +0.0 2,691,641 - -0.9 2,691,761 + SO2562 0.21 +1.0 2,691,769 - SO2562 0.23 +1.6 2,691,800 - SO2562 0.27 +2.3 2,691,850 - SO2562 0.34 +2.2 2,691,852 + SO2562 0.34 +1.8 2,691,857 + SO2562 0.35 +2.3 2,691,857 + SO2562 0.35 -1.1 2,691,860 - SO2562 0.35 +1.6 2,691,909 - SO2562 0.42 +1.6 2,691,909 - SO2562 0.42 +0.7 2,691,954 + SO2562 0.48 -0.3 2,692,312 - +0.3 2,692,319 + -0.2 2,692,321 + +3.2 2,692,329 - +1.1 2,692,408 - +0.3 2,692,501 - +1.7 2,692,579 - SO2563 0.10 +1.0 2,692,662 + SO2563 0.20 +2.8 2,692,664 + SO2563 0.21 +1.4 2,692,665 + SO2563 0.21 +1.3 2,692,723 + SO2563 0.28 +1.1 2,692,837 - SO2563 0.42 +3.1 2,692,853 - SO2563 0.44 -0.6 2,692,965 - SO2563 0.58 +2.3 2,692,981 - SO2563 0.60 +0.3 2,693,010 + SO2563 0.64 +1.7 2,693,082 + SO2563 0.73 -1.9 2,693,198 + SO2563 0.87 +0.8 2,693,210 + SO2563 0.89 +2.6 2,693,210 + SO2563 0.89 +2.3 2,693,210 + SO2563 0.89 +0.9 2,693,210 + SO2563 0.89 +1.9 2,693,246 + +2.1 2,693,273 + -0.0
Or see this region's nucleotide sequence