Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiC and SO2446 are separated by 435 nucleotides SO2446 and SO2447 are separated by 340 nucleotides
SO2445: thiC - thiamin biosynthesis protein ThiC (NCBI ptt file), at 2,558,537 to 2,560,702
thiC
SO2446: SO2446 - hypothetical protein (NCBI ptt file), at 2,561,138 to 2,562,205
SO2446
SO2447: SO2447 - channel protein, hemolysin III family subfamily (NCBI ptt file), at 2,562,546 to 2,563,199
SO2447
Position (kb)
2561
2562
2563 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2560.207 kb on - strand, within thiC at 2560.379 kb on - strand, within thiC at 2560.430 kb on - strand, within thiC at 2560.439 kb on + strand, within thiC at 2560.502 kb on - strand at 2560.614 kb on + strand at 2560.687 kb on + strand at 2560.687 kb on + strand at 2560.687 kb on + strand at 2560.687 kb on + strand at 2560.695 kb on - strand at 2560.750 kb on + strand at 2560.829 kb on - strand at 2560.937 kb on + strand at 2560.967 kb on + strand at 2561.166 kb on + strand at 2561.174 kb on - strand at 2561.246 kb on - strand, within SO2446 at 2561.251 kb on - strand, within SO2446 at 2561.343 kb on + strand, within SO2446 at 2561.367 kb on + strand, within SO2446 at 2561.402 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand at 2561.742 kb on + strand, within SO2446 at 2561.755 kb on + strand, within SO2446 at 2562.020 kb on - strand, within SO2446 at 2562.061 kb on + strand, within SO2446 at 2562.069 kb on - strand, within SO2446 at 2562.246 kb on + strand at 2562.268 kb on + strand at 2562.376 kb on + strand at 2562.496 kb on + strand at 2562.496 kb on + strand at 2562.504 kb on - strand at 2562.506 kb on + strand at 2562.506 kb on + strand at 2562.506 kb on + strand at 2562.506 kb on + strand at 2562.506 kb on + strand at 2562.506 kb on + strand at 2562.506 kb on + strand at 2562.514 kb on - strand at 2562.514 kb on - strand at 2562.514 kb on - strand at 2562.514 kb on - strand at 2562.514 kb on - strand at 2562.514 kb on - strand at 2562.514 kb on - strand at 2562.532 kb on + strand at 2562.619 kb on + strand, within SO2447 at 2562.854 kb on - strand, within SO2447 at 2562.862 kb on - strand, within SO2447 at 2562.878 kb on + strand, within SO2447 at 2563.089 kb on - strand, within SO2447 at 2563.089 kb on - strand, within SO2447 at 2563.101 kb on - strand, within SO2447
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,560,207 - thiC SO2445 0.77 +0.5 2,560,379 - thiC SO2445 0.85 -1.1 2,560,430 - thiC SO2445 0.87 -1.0 2,560,439 + thiC SO2445 0.88 -0.6 2,560,502 - -0.1 2,560,614 + -0.8 2,560,687 + -0.0 2,560,687 + +0.4 2,560,687 + -1.0 2,560,687 + -0.6 2,560,695 - +0.7 2,560,750 + -1.6 2,560,829 - -0.0 2,560,937 + -1.6 2,560,967 + +0.7 2,561,166 + -0.2 2,561,174 - +0.7 2,561,246 - SO2446 0.10 -2.0 2,561,251 - SO2446 0.11 +0.4 2,561,343 + SO2446 0.19 +0.0 2,561,367 + SO2446 0.21 +0.4 2,561,402 + SO2446 0.25 -2.9 2,561,553 + SO2446 0.39 -2.5 2,561,553 + SO2446 0.39 -0.0 2,561,553 + SO2446 0.39 -0.0 2,561,553 + SO2446 0.39 -2.3 2,561,553 + SO2446 0.39 -2.6 2,561,553 + +1.0 2,561,742 + SO2446 0.57 +0.7 2,561,755 + SO2446 0.58 +1.3 2,562,020 - SO2446 0.83 -0.2 2,562,061 + SO2446 0.86 -0.2 2,562,069 - SO2446 0.87 +1.3 2,562,246 + -0.2 2,562,268 + -0.0 2,562,376 + +0.4 2,562,496 + +1.1 2,562,496 + -1.0 2,562,504 - -0.0 2,562,506 + +1.1 2,562,506 + +1.0 2,562,506 + -0.0 2,562,506 + -1.0 2,562,506 + -1.0 2,562,506 + +0.1 2,562,506 + +2.0 2,562,514 - -1.5 2,562,514 - +2.0 2,562,514 - +1.2 2,562,514 - +1.1 2,562,514 - +1.2 2,562,514 - -0.4 2,562,514 - +2.0 2,562,532 + -0.5 2,562,619 + SO2447 0.11 -0.6 2,562,854 - SO2447 0.47 +1.5 2,562,862 - SO2447 0.48 -0.9 2,562,878 + SO2447 0.51 -0.3 2,563,089 - SO2447 0.83 +1.0 2,563,089 - SO2447 0.83 +1.6 2,563,101 - SO2447 0.85 +1.5
Or see this region's nucleotide sequence