Strain Fitness in Shewanella oneidensis MR-1 around SO2390

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntemrD and pssA are separated by 70 nucleotidespssA and SO2391 are separated by 11 nucleotidesSO2391 and SO2392 are separated by 203 nucleotides SO2389: emrD - multidrug resistance protein D (NCBI ptt file), at 2,492,556 to 2,493,800 emrD SO2390: pssA - CDP-diacylglycerol--serine O-phosphatidyltransferase (NCBI ptt file), at 2,493,871 to 2,495,184 pssA SO2391: SO2391 - conserved hypothetical protein (NCBI ptt file), at 2,495,196 to 2,495,528 SO2391 SO2392: SO2392 - ISSod4, transposase (NCBI ptt file), at 2,495,732 to 2,496,934 SO2392 Position (kb) 2493 2494 2495 2496Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5 6 7at 2492.927 kb on + strand, within emrDat 2493.074 kb on - strand, within emrDat 2493.145 kb on - strand, within emrDat 2493.178 kb on - strand, within emrDat 2493.200 kb on - strand, within emrDat 2493.213 kb on + strand, within emrDat 2493.229 kb on + strand, within emrDat 2493.433 kb on - strand, within emrDat 2493.441 kb on - strand, within emrDat 2493.456 kb on - strand, within emrDat 2493.456 kb on - strand, within emrDat 2493.472 kb on + strand, within emrDat 2493.558 kb on + strand, within emrDat 2493.571 kb on - strand, within emrDat 2493.654 kb on + strand, within emrDat 2493.712 kb on + strandat 2493.712 kb on + strandat 2493.746 kb on - strandat 2494.574 kb on - strand, within pssAat 2494.894 kb on - strand, within pssAat 2495.201 kb on - strandat 2495.631 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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2,492,927 + emrD SO2389 0.30 -0.8
2,493,074 - emrD SO2389 0.42 -1.5
2,493,145 - emrD SO2389 0.47 -2.8
2,493,178 - emrD SO2389 0.50 -2.0
2,493,200 - emrD SO2389 0.52 +0.0
2,493,213 + emrD SO2389 0.53 -1.6
2,493,229 + emrD SO2389 0.54 -2.2
2,493,433 - emrD SO2389 0.70 +0.7
2,493,441 - emrD SO2389 0.71 -1.5
2,493,456 - emrD SO2389 0.72 -0.4
2,493,456 - emrD SO2389 0.72 -4.1
2,493,472 + emrD SO2389 0.74 -0.1
2,493,558 + emrD SO2389 0.80 -1.0
2,493,571 - emrD SO2389 0.82 +0.5
2,493,654 + emrD SO2389 0.88 -1.1
2,493,712 + -0.4
2,493,712 + -2.6
2,493,746 - -0.4
2,494,574 - pssA SO2390 0.54 +7.0
2,494,894 - pssA SO2390 0.78 +7.6
2,495,201 - +1.4
2,495,631 - +1.1

Or see this region's nucleotide sequence