Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt emrD and pssA are separated by 70 nucleotides pssA and SO2391 are separated by 11 nucleotides SO2391 and SO2392 are separated by 203 nucleotides
SO2389: emrD - multidrug resistance protein D (NCBI ptt file), at 2,492,556 to 2,493,800
emrD
SO2390: pssA - CDP-diacylglycerol--serine O-phosphatidyltransferase (NCBI ptt file), at 2,493,871 to 2,495,184
pssA
SO2391: SO2391 - conserved hypothetical protein (NCBI ptt file), at 2,495,196 to 2,495,528
SO2391
SO2392: SO2392 - ISSod4, transposase (NCBI ptt file), at 2,495,732 to 2,496,934
SO2392
Position (kb)
2493
2494
2495
2496 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 2492.927 kb on + strand, within emrD at 2493.074 kb on - strand, within emrD at 2493.145 kb on - strand, within emrD at 2493.178 kb on - strand, within emrD at 2493.200 kb on - strand, within emrD at 2493.213 kb on + strand, within emrD at 2493.229 kb on + strand, within emrD at 2493.433 kb on - strand, within emrD at 2493.441 kb on - strand, within emrD at 2493.456 kb on - strand, within emrD at 2493.456 kb on - strand, within emrD at 2493.472 kb on + strand, within emrD at 2493.558 kb on + strand, within emrD at 2493.571 kb on - strand, within emrD at 2493.654 kb on + strand, within emrD at 2493.712 kb on + strand at 2493.712 kb on + strand at 2493.746 kb on - strand at 2494.574 kb on - strand, within pssA at 2494.894 kb on - strand, within pssA at 2495.201 kb on - strand at 2495.631 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,492,927 + emrD SO2389 0.30 -0.8 2,493,074 - emrD SO2389 0.42 -1.5 2,493,145 - emrD SO2389 0.47 -2.8 2,493,178 - emrD SO2389 0.50 -2.0 2,493,200 - emrD SO2389 0.52 +0.0 2,493,213 + emrD SO2389 0.53 -1.6 2,493,229 + emrD SO2389 0.54 -2.2 2,493,433 - emrD SO2389 0.70 +0.7 2,493,441 - emrD SO2389 0.71 -1.5 2,493,456 - emrD SO2389 0.72 -0.4 2,493,456 - emrD SO2389 0.72 -4.1 2,493,472 + emrD SO2389 0.74 -0.1 2,493,558 + emrD SO2389 0.80 -1.0 2,493,571 - emrD SO2389 0.82 +0.5 2,493,654 + emrD SO2389 0.88 -1.1 2,493,712 + -0.4 2,493,712 + -2.6 2,493,746 - -0.4 2,494,574 - pssA SO2390 0.54 +7.0 2,494,894 - pssA SO2390 0.78 +7.6 2,495,201 - +1.4 2,495,631 - +1.1
Or see this region's nucleotide sequence