Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2380 and SO2381 are separated by 78 nucleotides SO2381 and SO2383 are separated by 262 nucleotides
SO2380: SO2380 - RecQ domain protein (NCBI ptt file), at 2,482,458 to 2,484,422
SO2380
SO2381: SO2381 - hypothetical protein (NCBI ptt file), at 2,484,501 to 2,484,719
SO2381
SO2383: SO2383 - hypothetical protein (NCBI ptt file), at 2,484,982 to 2,485,485
SO2383
Position (kb)
2484
2485 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2483.617 kb on + strand, within SO2380 at 2483.625 kb on - strand, within SO2380 at 2483.771 kb on - strand, within SO2380 at 2483.878 kb on + strand, within SO2380 at 2483.979 kb on - strand, within SO2380 at 2484.386 kb on - strand at 2484.424 kb on + strand at 2484.460 kb on + strand at 2484.486 kb on + strand at 2484.548 kb on - strand, within SO2381 at 2484.775 kb on + strand at 2484.782 kb on - strand at 2484.803 kb on - strand at 2484.874 kb on - strand at 2484.880 kb on + strand at 2484.891 kb on - strand at 2484.902 kb on + strand at 2485.056 kb on + strand, within SO2383 at 2485.064 kb on - strand, within SO2383 at 2485.066 kb on + strand, within SO2383 at 2485.074 kb on - strand, within SO2383 at 2485.074 kb on - strand, within SO2383 at 2485.088 kb on - strand, within SO2383 at 2485.155 kb on - strand, within SO2383 at 2485.176 kb on + strand, within SO2383 at 2485.176 kb on + strand, within SO2383 at 2485.227 kb on + strand, within SO2383 at 2485.291 kb on + strand, within SO2383 at 2485.297 kb on - strand, within SO2383 at 2485.299 kb on - strand, within SO2383 at 2485.301 kb on + strand, within SO2383 at 2485.308 kb on + strand, within SO2383 at 2485.309 kb on - strand, within SO2383 at 2485.316 kb on - strand, within SO2383 at 2485.316 kb on - strand, within SO2383 at 2485.316 kb on - strand, within SO2383 at 2485.336 kb on + strand, within SO2383 at 2485.336 kb on - strand, within SO2383 at 2485.343 kb on + strand, within SO2383 at 2485.350 kb on + strand, within SO2383 at 2485.351 kb on + strand, within SO2383 at 2485.351 kb on - strand, within SO2383 at 2485.351 kb on - strand, within SO2383 at 2485.351 kb on - strand, within SO2383 at 2485.359 kb on + strand, within SO2383 at 2485.367 kb on - strand, within SO2383 at 2485.446 kb on + strand at 2485.446 kb on + strand at 2485.637 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,483,617 + SO2380 0.59 +1.2 2,483,625 - SO2380 0.59 -2.4 2,483,771 - SO2380 0.67 +1.1 2,483,878 + SO2380 0.72 +0.8 2,483,979 - SO2380 0.77 +0.4 2,484,386 - +1.1 2,484,424 + -0.3 2,484,460 + +1.5 2,484,486 + -0.8 2,484,548 - SO2381 0.21 +1.5 2,484,775 + +1.0 2,484,782 - +0.0 2,484,803 - +0.6 2,484,874 - +0.3 2,484,880 + -1.4 2,484,891 - +0.1 2,484,902 + +0.4 2,485,056 + SO2383 0.15 +1.4 2,485,064 - SO2383 0.16 +0.5 2,485,066 + SO2383 0.17 +1.3 2,485,074 - SO2383 0.18 -1.1 2,485,074 - SO2383 0.18 -3.1 2,485,088 - SO2383 0.21 +0.7 2,485,155 - SO2383 0.34 -0.7 2,485,176 + SO2383 0.38 +0.4 2,485,176 + SO2383 0.38 +0.2 2,485,227 + SO2383 0.49 +1.7 2,485,291 + SO2383 0.61 -0.4 2,485,297 - SO2383 0.62 -1.0 2,485,299 - SO2383 0.63 +1.0 2,485,301 + SO2383 0.63 -1.1 2,485,308 + SO2383 0.65 -0.6 2,485,309 - SO2383 0.65 +2.5 2,485,316 - SO2383 0.66 +1.5 2,485,316 - SO2383 0.66 +2.3 2,485,316 - SO2383 0.66 +0.4 2,485,336 + SO2383 0.70 +1.0 2,485,336 - SO2383 0.70 -0.7 2,485,343 + SO2383 0.72 +1.1 2,485,350 + SO2383 0.73 -1.0 2,485,351 + SO2383 0.73 +1.2 2,485,351 - SO2383 0.73 -1.1 2,485,351 - SO2383 0.73 -1.0 2,485,351 - SO2383 0.73 -0.5 2,485,359 + SO2383 0.75 +0.0 2,485,367 - SO2383 0.76 -0.1 2,485,446 + +0.9 2,485,446 + -0.5 2,485,637 - +0.7
Or see this region's nucleotide sequence