Strain Fitness in Shewanella oneidensis MR-1 around SO2341

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2339 and SO2340 are separated by 3 nucleotidesSO2340 and SO2341 are separated by 9 nucleotidesSO2341 and nadA are separated by 134 nucleotides SO2339: SO2339 - alpha keto acid dehydrogenase complex, E1 component, alpha subunit (NCBI ptt file), at 2,446,372 to 2,447,550 SO2339 SO2340: SO2340 - alpha keto acid dehydrogenase complex, E1 component, beta subunit (NCBI ptt file), at 2,447,554 to 2,448,531 SO2340 SO2341: SO2341 - alpha keto acid dehydrogenase complex, E2 component (NCBI ptt file), at 2,448,541 to 2,450,148 SO2341 SO2342: nadA - quinolinate synthetase complex, subunit A (NCBI ptt file), at 2,450,283 to 2,451,356 nadA Position (kb) 2448 2449 2450 2451Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2447.675 kb on + strand, within SO2340at 2447.683 kb on - strand, within SO2340at 2447.731 kb on + strand, within SO2340at 2447.739 kb on - strand, within SO2340at 2447.773 kb on + strand, within SO2340at 2447.870 kb on - strand, within SO2340at 2447.876 kb on + strand, within SO2340at 2447.876 kb on + strand, within SO2340at 2447.884 kb on - strand, within SO2340at 2447.891 kb on + strand, within SO2340at 2447.899 kb on - strand, within SO2340at 2447.937 kb on + strand, within SO2340at 2448.005 kb on + strand, within SO2340at 2448.008 kb on - strand, within SO2340at 2448.135 kb on + strand, within SO2340at 2448.158 kb on - strand, within SO2340at 2448.160 kb on + strand, within SO2340at 2448.160 kb on + strand, within SO2340at 2448.160 kb on + strand, within SO2340at 2448.160 kb on + strand, within SO2340at 2448.168 kb on - strand, within SO2340at 2448.168 kb on - strand, within SO2340at 2448.296 kb on - strand, within SO2340at 2448.418 kb on + strand, within SO2340at 2448.664 kb on + strandat 2448.764 kb on - strand, within SO2341at 2448.838 kb on + strand, within SO2341at 2449.005 kb on - strand, within SO2341at 2449.005 kb on - strand, within SO2341at 2449.005 kb on - strand, within SO2341at 2449.019 kb on - strand, within SO2341at 2449.059 kb on - strand, within SO2341at 2449.102 kb on + strand, within SO2341at 2449.221 kb on - strand, within SO2341at 2449.221 kb on - strand, within SO2341at 2449.316 kb on - strand, within SO2341at 2449.349 kb on - strand, within SO2341at 2449.404 kb on - strand, within SO2341at 2449.419 kb on - strand, within SO2341at 2449.485 kb on + strand, within SO2341at 2449.652 kb on + strand, within SO2341at 2449.982 kb on - strand, within SO2341at 2450.042 kb on + strandat 2450.261 kb on + strandat 2450.261 kb on + strandat 2450.261 kb on + strandat 2450.261 kb on + strandat 2450.898 kb on - strand, within nadAat 2451.012 kb on + strand, within nadAat 2451.037 kb on - strand, within nadAat 2451.037 kb on - strand, within nadA

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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2,447,675 + SO2340 0.12 -3.5
2,447,683 - SO2340 0.13 -4.3
2,447,731 + SO2340 0.18 -4.4
2,447,739 - SO2340 0.19 -1.9
2,447,773 + SO2340 0.22 -5.9
2,447,870 - SO2340 0.32 -2.9
2,447,876 + SO2340 0.33 -2.3
2,447,876 + SO2340 0.33 -3.8
2,447,884 - SO2340 0.34 -2.5
2,447,891 + SO2340 0.34 -4.6
2,447,899 - SO2340 0.35 -2.7
2,447,937 + SO2340 0.39 -4.6
2,448,005 + SO2340 0.46 -2.9
2,448,008 - SO2340 0.46 -4.8
2,448,135 + SO2340 0.59 -2.5
2,448,158 - SO2340 0.62 -3.9
2,448,160 + SO2340 0.62 -5.2
2,448,160 + SO2340 0.62 -5.1
2,448,160 + SO2340 0.62 -4.3
2,448,160 + SO2340 0.62 -3.4
2,448,168 - SO2340 0.63 -5.4
2,448,168 - SO2340 0.63 -2.7
2,448,296 - SO2340 0.76 -3.4
2,448,418 + SO2340 0.88 -3.4
2,448,664 + -2.8
2,448,764 - SO2341 0.14 -3.9
2,448,838 + SO2341 0.18 -3.1
2,449,005 - SO2341 0.29 -3.3
2,449,005 - SO2341 0.29 -5.5
2,449,005 - SO2341 0.29 -3.5
2,449,019 - SO2341 0.30 -2.3
2,449,059 - SO2341 0.32 -1.9
2,449,102 + SO2341 0.35 -2.9
2,449,221 - SO2341 0.42 -4.9
2,449,221 - SO2341 0.42 -2.5
2,449,316 - SO2341 0.48 -3.5
2,449,349 - SO2341 0.50 -4.4
2,449,404 - SO2341 0.54 -3.5
2,449,419 - SO2341 0.55 -3.3
2,449,485 + SO2341 0.59 -2.7
2,449,652 + SO2341 0.69 -4.1
2,449,982 - SO2341 0.90 -2.9
2,450,042 + -4.5
2,450,261 + -0.9
2,450,261 + -2.7
2,450,261 + -2.3
2,450,261 + -0.4
2,450,898 - nadA SO2342 0.57 -5.7
2,451,012 + nadA SO2342 0.68 -1.9
2,451,037 - nadA SO2342 0.70 -2.3
2,451,037 - nadA SO2342 0.70 -2.3

Or see this region's nucleotide sequence