Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2339 and SO2340 are separated by 3 nucleotides SO2340 and SO2341 are separated by 9 nucleotides SO2341 and nadA are separated by 134 nucleotides
SO2339: SO2339 - alpha keto acid dehydrogenase complex, E1 component, alpha subunit (NCBI ptt file), at 2,446,372 to 2,447,550
SO2339
SO2340: SO2340 - alpha keto acid dehydrogenase complex, E1 component, beta subunit (NCBI ptt file), at 2,447,554 to 2,448,531
SO2340
SO2341: SO2341 - alpha keto acid dehydrogenase complex, E2 component (NCBI ptt file), at 2,448,541 to 2,450,148
SO2341
SO2342: nadA - quinolinate synthetase complex, subunit A (NCBI ptt file), at 2,450,283 to 2,451,356
nadA
Position (kb)
2448
2449
2450
2451 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2447.675 kb on + strand, within SO2340 at 2447.683 kb on - strand, within SO2340 at 2447.731 kb on + strand, within SO2340 at 2447.739 kb on - strand, within SO2340 at 2447.773 kb on + strand, within SO2340 at 2447.870 kb on - strand, within SO2340 at 2447.876 kb on + strand, within SO2340 at 2447.876 kb on + strand, within SO2340 at 2447.884 kb on - strand, within SO2340 at 2447.891 kb on + strand, within SO2340 at 2447.899 kb on - strand, within SO2340 at 2447.937 kb on + strand, within SO2340 at 2448.005 kb on + strand, within SO2340 at 2448.008 kb on - strand, within SO2340 at 2448.135 kb on + strand, within SO2340 at 2448.158 kb on - strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.168 kb on - strand, within SO2340 at 2448.168 kb on - strand, within SO2340 at 2448.296 kb on - strand, within SO2340 at 2448.418 kb on + strand, within SO2340 at 2448.664 kb on + strand at 2448.764 kb on - strand, within SO2341 at 2448.838 kb on + strand, within SO2341 at 2449.005 kb on - strand, within SO2341 at 2449.005 kb on - strand, within SO2341 at 2449.005 kb on - strand, within SO2341 at 2449.019 kb on - strand, within SO2341 at 2449.059 kb on - strand, within SO2341 at 2449.102 kb on + strand, within SO2341 at 2449.221 kb on - strand, within SO2341 at 2449.221 kb on - strand, within SO2341 at 2449.316 kb on - strand, within SO2341 at 2449.349 kb on - strand, within SO2341 at 2449.404 kb on - strand, within SO2341 at 2449.419 kb on - strand, within SO2341 at 2449.485 kb on + strand, within SO2341 at 2449.652 kb on + strand, within SO2341 at 2449.982 kb on - strand, within SO2341 at 2450.042 kb on + strand at 2450.261 kb on + strand at 2450.261 kb on + strand at 2450.261 kb on + strand at 2450.261 kb on + strand at 2450.898 kb on - strand, within nadA at 2451.012 kb on + strand, within nadA at 2451.037 kb on - strand, within nadA at 2451.037 kb on - strand, within nadA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,447,675 + SO2340 0.12 -3.5 2,447,683 - SO2340 0.13 -4.3 2,447,731 + SO2340 0.18 -4.4 2,447,739 - SO2340 0.19 -1.9 2,447,773 + SO2340 0.22 -5.9 2,447,870 - SO2340 0.32 -2.9 2,447,876 + SO2340 0.33 -2.3 2,447,876 + SO2340 0.33 -3.8 2,447,884 - SO2340 0.34 -2.5 2,447,891 + SO2340 0.34 -4.6 2,447,899 - SO2340 0.35 -2.7 2,447,937 + SO2340 0.39 -4.6 2,448,005 + SO2340 0.46 -2.9 2,448,008 - SO2340 0.46 -4.8 2,448,135 + SO2340 0.59 -2.5 2,448,158 - SO2340 0.62 -3.9 2,448,160 + SO2340 0.62 -5.2 2,448,160 + SO2340 0.62 -5.1 2,448,160 + SO2340 0.62 -4.3 2,448,160 + SO2340 0.62 -3.4 2,448,168 - SO2340 0.63 -5.4 2,448,168 - SO2340 0.63 -2.7 2,448,296 - SO2340 0.76 -3.4 2,448,418 + SO2340 0.88 -3.4 2,448,664 + -2.8 2,448,764 - SO2341 0.14 -3.9 2,448,838 + SO2341 0.18 -3.1 2,449,005 - SO2341 0.29 -3.3 2,449,005 - SO2341 0.29 -5.5 2,449,005 - SO2341 0.29 -3.5 2,449,019 - SO2341 0.30 -2.3 2,449,059 - SO2341 0.32 -1.9 2,449,102 + SO2341 0.35 -2.9 2,449,221 - SO2341 0.42 -4.9 2,449,221 - SO2341 0.42 -2.5 2,449,316 - SO2341 0.48 -3.5 2,449,349 - SO2341 0.50 -4.4 2,449,404 - SO2341 0.54 -3.5 2,449,419 - SO2341 0.55 -3.3 2,449,485 + SO2341 0.59 -2.7 2,449,652 + SO2341 0.69 -4.1 2,449,982 - SO2341 0.90 -2.9 2,450,042 + -4.5 2,450,261 + -0.9 2,450,261 + -2.7 2,450,261 + -2.3 2,450,261 + -0.4 2,450,898 - nadA SO2342 0.57 -5.7 2,451,012 + nadA SO2342 0.68 -1.9 2,451,037 - nadA SO2342 0.70 -2.3 2,451,037 - nadA SO2342 0.70 -2.3
Or see this region's nucleotide sequence