Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt astE and SO2339 are separated by 162 nucleotides SO2339 and SO2340 are separated by 3 nucleotides SO2340 and SO2341 are separated by 9 nucleotides
SO2338: astE - succinylglutamate desuccinylase (NCBI ptt file), at 2,445,175 to 2,446,209
astE
SO2339: SO2339 - alpha keto acid dehydrogenase complex, E1 component, alpha subunit (NCBI ptt file), at 2,446,372 to 2,447,550
SO2339
SO2340: SO2340 - alpha keto acid dehydrogenase complex, E1 component, beta subunit (NCBI ptt file), at 2,447,554 to 2,448,531
SO2340
SO2341: SO2341 - alpha keto acid dehydrogenase complex, E2 component (NCBI ptt file), at 2,448,541 to 2,450,148
SO2341
Position (kb)
2446
2447
2448 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2445.527 kb on - strand, within astE at 2445.534 kb on + strand, within astE at 2445.560 kb on - strand, within astE at 2445.730 kb on - strand, within astE at 2445.732 kb on + strand, within astE at 2445.839 kb on + strand, within astE at 2445.863 kb on - strand, within astE at 2445.934 kb on + strand, within astE at 2446.184 kb on + strand at 2446.818 kb on - strand, within SO2339 at 2446.862 kb on + strand, within SO2339 at 2446.868 kb on - strand, within SO2339 at 2446.968 kb on - strand, within SO2339 at 2447.090 kb on - strand, within SO2339 at 2447.151 kb on + strand, within SO2339 at 2447.160 kb on - strand, within SO2339 at 2447.196 kb on + strand, within SO2339 at 2447.420 kb on - strand, within SO2339 at 2447.675 kb on + strand, within SO2340 at 2447.683 kb on - strand, within SO2340 at 2447.731 kb on + strand, within SO2340 at 2447.739 kb on - strand, within SO2340 at 2447.773 kb on + strand, within SO2340 at 2447.870 kb on - strand, within SO2340 at 2447.876 kb on + strand, within SO2340 at 2447.876 kb on + strand, within SO2340 at 2447.884 kb on - strand, within SO2340 at 2447.891 kb on + strand, within SO2340 at 2447.899 kb on - strand, within SO2340 at 2447.937 kb on + strand, within SO2340 at 2448.005 kb on + strand, within SO2340 at 2448.008 kb on - strand, within SO2340 at 2448.135 kb on + strand, within SO2340 at 2448.158 kb on - strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.168 kb on - strand, within SO2340 at 2448.168 kb on - strand, within SO2340 at 2448.296 kb on - strand, within SO2340 at 2448.418 kb on + strand, within SO2340
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,445,527 - astE SO2338 0.34 -1.9 2,445,534 + astE SO2338 0.35 -4.8 2,445,560 - astE SO2338 0.37 -4.6 2,445,730 - astE SO2338 0.54 -1.6 2,445,732 + astE SO2338 0.54 -3.3 2,445,839 + astE SO2338 0.64 -3.1 2,445,863 - astE SO2338 0.66 -2.2 2,445,934 + astE SO2338 0.73 -3.2 2,446,184 + +1.5 2,446,818 - SO2339 0.38 -4.2 2,446,862 + SO2339 0.42 -3.5 2,446,868 - SO2339 0.42 -1.9 2,446,968 - SO2339 0.51 -3.6 2,447,090 - SO2339 0.61 -3.1 2,447,151 + SO2339 0.66 -2.7 2,447,160 - SO2339 0.67 -5.0 2,447,196 + SO2339 0.70 -3.3 2,447,420 - SO2339 0.89 -4.7 2,447,675 + SO2340 0.12 -3.5 2,447,683 - SO2340 0.13 -4.3 2,447,731 + SO2340 0.18 -4.4 2,447,739 - SO2340 0.19 -1.9 2,447,773 + SO2340 0.22 -5.9 2,447,870 - SO2340 0.32 -2.9 2,447,876 + SO2340 0.33 -2.3 2,447,876 + SO2340 0.33 -3.8 2,447,884 - SO2340 0.34 -2.5 2,447,891 + SO2340 0.34 -4.6 2,447,899 - SO2340 0.35 -2.7 2,447,937 + SO2340 0.39 -4.6 2,448,005 + SO2340 0.46 -2.9 2,448,008 - SO2340 0.46 -4.8 2,448,135 + SO2340 0.59 -2.5 2,448,158 - SO2340 0.62 -3.9 2,448,160 + SO2340 0.62 -5.2 2,448,160 + SO2340 0.62 -5.1 2,448,160 + SO2340 0.62 -4.3 2,448,160 + SO2340 0.62 -3.4 2,448,168 - SO2340 0.63 -5.4 2,448,168 - SO2340 0.63 -2.7 2,448,296 - SO2340 0.76 -3.4 2,448,418 + SO2340 0.88 -3.4
Or see this region's nucleotide sequence