Strain Fitness in Shewanella oneidensis MR-1 around SO2333

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2330 and SO2331 are separated by 55 nucleotidesSO2331 and SO2332 are separated by 11 nucleotidesSO2332 and SO2333 are separated by 101 nucleotidesSO2333 and seqA are separated by 230 nucleotidesseqA and pgm are separated by 70 nucleotides SO2330: SO2330 - flavodoxin (NCBI ptt file), at 2,439,769 to 2,440,296 SO2330 SO2331: SO2331 - conserved hypothetical protein (NCBI ptt file), at 2,440,352 to 2,440,624 SO2331 SO2332: SO2332 - conserved hypothetical protein (NCBI ptt file), at 2,440,636 to 2,440,857 SO2332 SO2333: SO2333 - hydrolase, alpha/beta fold family (NCBI ptt file), at 2,440,959 to 2,441,801 SO2333 SO2335: seqA - seqA protein (NCBI ptt file), at 2,442,032 to 2,442,583 seqA SO2336: pgm - phosphoglucomutase, alpha-D-glucose phosphate-specific (NCBI ptt file), at 2,442,654 to 2,444,306 pgm Position (kb) 2440 2441 2442Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7at 2440.606 kb on - strandat 2440.676 kb on - strand, within SO2332at 2440.753 kb on - strand, within SO2332at 2440.869 kb on + strandat 2440.882 kb on - strandat 2440.956 kb on + strandat 2441.308 kb on + strand, within SO2333at 2441.521 kb on - strand, within SO2333at 2441.524 kb on + strand, within SO2333at 2441.547 kb on + strand, within SO2333at 2441.553 kb on + strand, within SO2333at 2441.617 kb on - strand, within SO2333at 2441.649 kb on + strand, within SO2333at 2441.756 kb on + strandat 2441.756 kb on + strandat 2441.764 kb on - strandat 2441.810 kb on - strandat 2441.945 kb on + strandat 2441.950 kb on + strandat 2442.767 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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2,440,606 - -2.2
2,440,676 - SO2332 0.18 +1.0
2,440,753 - SO2332 0.53 -1.3
2,440,869 + -0.0
2,440,882 - -0.6
2,440,956 + -0.1
2,441,308 + SO2333 0.41 -3.6
2,441,521 - SO2333 0.67 -4.1
2,441,524 + SO2333 0.67 -4.1
2,441,547 + SO2333 0.70 -3.8
2,441,553 + SO2333 0.70 -2.3
2,441,617 - SO2333 0.78 -5.2
2,441,649 + SO2333 0.82 -4.8
2,441,756 + -2.5
2,441,756 + -2.3
2,441,764 - -0.9
2,441,810 - -2.3
2,441,945 + +0.6
2,441,950 + -2.9
2,442,767 - +7.1

Or see this region's nucleotide sequence