Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2330 and SO2331 are separated by 55 nucleotides SO2331 and SO2332 are separated by 11 nucleotides SO2332 and SO2333 are separated by 101 nucleotides SO2333 and seqA are separated by 230 nucleotides seqA and pgm are separated by 70 nucleotides
SO2330: SO2330 - flavodoxin (NCBI ptt file), at 2,439,769 to 2,440,296
SO2330
SO2331: SO2331 - conserved hypothetical protein (NCBI ptt file), at 2,440,352 to 2,440,624
SO2331
SO2332: SO2332 - conserved hypothetical protein (NCBI ptt file), at 2,440,636 to 2,440,857
SO2332
SO2333: SO2333 - hydrolase, alpha/beta fold family (NCBI ptt file), at 2,440,959 to 2,441,801
SO2333
SO2335: seqA - seqA protein (NCBI ptt file), at 2,442,032 to 2,442,583
seqA
SO2336: pgm - phosphoglucomutase, alpha-D-glucose phosphate-specific (NCBI ptt file), at 2,442,654 to 2,444,306
pgm
Position (kb)
2440
2441
2442 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 2440.606 kb on - strand at 2440.676 kb on - strand, within SO2332 at 2440.753 kb on - strand, within SO2332 at 2440.869 kb on + strand at 2440.882 kb on - strand at 2440.956 kb on + strand at 2441.308 kb on + strand, within SO2333 at 2441.521 kb on - strand, within SO2333 at 2441.524 kb on + strand, within SO2333 at 2441.547 kb on + strand, within SO2333 at 2441.553 kb on + strand, within SO2333 at 2441.617 kb on - strand, within SO2333 at 2441.649 kb on + strand, within SO2333 at 2441.756 kb on + strand at 2441.756 kb on + strand at 2441.764 kb on - strand at 2441.810 kb on - strand at 2441.945 kb on + strand at 2441.950 kb on + strand at 2442.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,440,606 - -2.2 2,440,676 - SO2332 0.18 +1.0 2,440,753 - SO2332 0.53 -1.3 2,440,869 + -0.0 2,440,882 - -0.6 2,440,956 + -0.1 2,441,308 + SO2333 0.41 -3.6 2,441,521 - SO2333 0.67 -4.1 2,441,524 + SO2333 0.67 -4.1 2,441,547 + SO2333 0.70 -3.8 2,441,553 + SO2333 0.70 -2.3 2,441,617 - SO2333 0.78 -5.2 2,441,649 + SO2333 0.82 -4.8 2,441,756 + -2.5 2,441,756 + -2.3 2,441,764 - -0.9 2,441,810 - -2.3 2,441,945 + +0.6 2,441,950 + -2.9 2,442,767 - +7.1
Or see this region's nucleotide sequence