Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2321 and SO_2322 are separated by 173 nucleotides SO_2322 and SO2323 are separated by 25 nucleotides SO2323 and cheW-2 are separated by 13 nucleotides cheW-2 and cheR-3 overlap by 4 nucleotides
SO2321: SO2321 - ISSod4, transposase (NCBI ptt file), at 2,430,667 to 2,431,869
SO2321
SO_2322: SO_2322 - no description, at 2,432,043 to 2,432,810
_2322
SO2323: SO2323 - methyl-accepting chemotaxis protein (NCBI ptt file), at 2,432,836 to 2,434,776
SO2323
SO2324: cheW-2 - purine-binding chemotaxis protein CheW (NCBI ptt file), at 2,434,790 to 2,435,326
cheW-2
SO2325: cheR-3 - chemotaxis protein methyltransferase CheR (NCBI ptt file), at 2,435,323 to 2,436,150
cheR-3
Position (kb)
2432
2433
2434
2435 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2431.898 kb on + strand at 2431.898 kb on + strand at 2431.910 kb on + strand at 2431.917 kb on - strand at 2431.987 kb on + strand at 2431.987 kb on + strand at 2431.995 kb on - strand at 2431.995 kb on - strand at 2432.000 kb on - strand at 2432.093 kb on + strand at 2432.105 kb on + strand at 2432.105 kb on + strand at 2432.105 kb on + strand at 2432.111 kb on - strand at 2432.113 kb on - strand at 2432.113 kb on - strand at 2432.113 kb on - strand at 2432.118 kb on - strand at 2432.118 kb on - strand at 2432.147 kb on - strand, within SO_2322 at 2432.174 kb on - strand, within SO_2322 at 2432.251 kb on + strand, within SO_2322 at 2432.296 kb on + strand, within SO_2322 at 2432.329 kb on - strand, within SO_2322 at 2432.329 kb on - strand, within SO_2322 at 2432.392 kb on - strand, within SO_2322 at 2432.397 kb on + strand, within SO_2322 at 2432.428 kb on - strand, within SO_2322 at 2432.447 kb on + strand, within SO_2322 at 2432.451 kb on - strand, within SO_2322 at 2432.482 kb on + strand, within SO_2322 at 2432.482 kb on + strand, within SO_2322 at 2432.490 kb on - strand, within SO_2322 at 2432.554 kb on + strand, within SO_2322 at 2432.572 kb on + strand, within SO_2322 at 2432.577 kb on + strand, within SO_2322 at 2432.597 kb on + strand, within SO_2322 at 2432.678 kb on + strand, within SO_2322 at 2432.685 kb on + strand, within SO_2322 at 2432.685 kb on + strand, within SO_2322 at 2432.685 kb on + strand, within SO_2322 at 2432.685 kb on + strand, within SO_2322 at 2432.685 kb on + strand, within SO_2322 at 2432.685 kb on + strand, within SO_2322 at 2432.687 kb on - strand, within SO_2322 at 2432.687 kb on - strand, within SO_2322 at 2432.692 kb on - strand, within SO_2322 at 2432.693 kb on - strand, within SO_2322 at 2432.693 kb on - strand, within SO_2322 at 2432.693 kb on - strand, within SO_2322 at 2432.725 kb on + strand, within SO_2322 at 2432.731 kb on + strand, within SO_2322 at 2432.732 kb on - strand, within SO_2322 at 2432.732 kb on - strand, within SO_2322 at 2432.739 kb on - strand at 2432.798 kb on + strand at 2432.934 kb on + strand at 2432.934 kb on + strand at 2432.934 kb on + strand at 2432.942 kb on - strand at 2432.942 kb on - strand at 2432.950 kb on - strand at 2432.976 kb on - strand at 2432.976 kb on - strand at 2432.981 kb on + strand at 2432.989 kb on - strand at 2433.008 kb on - strand at 2433.022 kb on + strand at 2433.030 kb on - strand, within SO2323 at 2433.076 kb on + strand, within SO2323 at 2433.169 kb on - strand, within SO2323 at 2433.203 kb on - strand, within SO2323 at 2433.255 kb on - strand, within SO2323 at 2433.262 kb on + strand, within SO2323 at 2433.269 kb on + strand, within SO2323 at 2433.269 kb on + strand, within SO2323 at 2433.269 kb on + strand, within SO2323 at 2433.277 kb on - strand, within SO2323 at 2433.277 kb on - strand, within SO2323 at 2433.277 kb on - strand, within SO2323 at 2433.277 kb on - strand, within SO2323 at 2433.277 kb on - strand, within SO2323 at 2433.304 kb on + strand, within SO2323 at 2433.304 kb on + strand, within SO2323 at 2433.311 kb on - strand, within SO2323 at 2433.312 kb on - strand, within SO2323 at 2433.344 kb on - strand, within SO2323 at 2433.355 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.362 kb on + strand, within SO2323 at 2433.367 kb on + strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.370 kb on - strand, within SO2323 at 2433.407 kb on - strand, within SO2323 at 2433.433 kb on + strand, within SO2323 at 2433.626 kb on + strand, within SO2323 at 2433.626 kb on + strand, within SO2323 at 2433.626 kb on + strand, within SO2323 at 2433.626 kb on + strand, within SO2323 at 2433.626 kb on + strand, within SO2323 at 2433.626 kb on + strand, within SO2323 at 2433.629 kb on - strand, within SO2323 at 2433.634 kb on - strand, within SO2323 at 2433.634 kb on - strand, within SO2323 at 2433.634 kb on - strand, within SO2323 at 2433.634 kb on - strand, within SO2323 at 2433.634 kb on - strand, within SO2323 at 2433.634 kb on - strand, within SO2323 at 2433.634 kb on - strand, within SO2323 at 2433.686 kb on + strand, within SO2323 at 2433.686 kb on + strand, within SO2323 at 2433.694 kb on - strand, within SO2323 at 2433.720 kb on + strand, within SO2323 at 2433.720 kb on + strand, within SO2323 at 2433.729 kb on - strand, within SO2323 at 2433.729 kb on - strand, within SO2323 at 2433.729 kb on - strand, within SO2323 at 2433.729 kb on - strand, within SO2323 at 2433.823 kb on - strand, within SO2323 at 2433.878 kb on + strand, within SO2323 at 2433.878 kb on + strand, within SO2323 at 2433.878 kb on + strand, within SO2323 at 2433.886 kb on - strand, within SO2323 at 2433.905 kb on + strand, within SO2323 at 2433.925 kb on + strand, within SO2323 at 2433.969 kb on - strand, within SO2323 at 2433.984 kb on + strand, within SO2323 at 2433.995 kb on + strand, within SO2323 at 2434.002 kb on + strand, within SO2323 at 2434.002 kb on + strand, within SO2323 at 2434.010 kb on - strand, within SO2323 at 2434.037 kb on + strand, within SO2323 at 2434.045 kb on - strand, within SO2323 at 2434.063 kb on - strand, within SO2323 at 2434.107 kb on - strand, within SO2323 at 2434.160 kb on + strand, within SO2323 at 2434.169 kb on - strand, within SO2323 at 2434.180 kb on + strand, within SO2323 at 2434.295 kb on + strand, within SO2323 at 2434.316 kb on + strand, within SO2323 at 2434.317 kb on - strand, within SO2323 at 2434.331 kb on + strand, within SO2323 at 2434.331 kb on + strand, within SO2323 at 2434.339 kb on - strand, within SO2323 at 2434.559 kb on - strand, within SO2323 at 2434.640 kb on + strand at 2434.684 kb on + strand at 2434.789 kb on - strand at 2434.923 kb on + strand at 2434.923 kb on + strand, within cheW-2 at 2435.186 kb on + strand, within cheW-2 at 2435.208 kb on + strand, within cheW-2 at 2435.214 kb on - strand, within cheW-2 at 2435.214 kb on - strand, within cheW-2 at 2435.227 kb on - strand, within cheW-2 at 2435.254 kb on + strand, within cheW-2 at 2435.325 kb on + strand at 2435.328 kb on - strand at 2435.328 kb on - strand at 2435.342 kb on + strand at 2435.356 kb on + strand at 2435.364 kb on - strand at 2435.448 kb on + strand, within cheR-3 at 2435.694 kb on + strand, within cheR-3 at 2435.697 kb on - strand, within cheR-3 at 2435.726 kb on - strand, within cheR-3 at 2435.753 kb on + strand, within cheR-3 at 2435.753 kb on - strand, within cheR-3 at 2435.761 kb on - strand, within cheR-3 at 2435.772 kb on + strand, within cheR-3
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,431,898 + +0.5 2,431,898 + -0.3 2,431,910 + +1.9 2,431,917 - -0.3 2,431,987 + +1.1 2,431,987 + -3.1 2,431,995 - +0.5 2,431,995 - +0.5 2,432,000 - +0.6 2,432,093 + -0.9 2,432,105 + +0.8 2,432,105 + -0.1 2,432,105 + +0.1 2,432,111 - +0.5 2,432,113 - +0.1 2,432,113 - +0.9 2,432,113 - +0.4 2,432,118 - +0.5 2,432,118 - -0.2 2,432,147 - SO_2322 0.14 -1.7 2,432,174 - SO_2322 0.17 +1.5 2,432,251 + SO_2322 0.27 +3.3 2,432,296 + SO_2322 0.33 +0.3 2,432,329 - SO_2322 0.37 -0.7 2,432,329 - SO_2322 0.37 +1.5 2,432,392 - SO_2322 0.45 +1.3 2,432,397 + SO_2322 0.46 +0.6 2,432,428 - SO_2322 0.50 +0.8 2,432,447 + SO_2322 0.53 -1.2 2,432,451 - SO_2322 0.53 +0.2 2,432,482 + SO_2322 0.57 +0.7 2,432,482 + SO_2322 0.57 -2.3 2,432,490 - SO_2322 0.58 -0.9 2,432,554 + SO_2322 0.67 -0.6 2,432,572 + SO_2322 0.69 -2.9 2,432,577 + SO_2322 0.70 +1.9 2,432,597 + SO_2322 0.72 +1.3 2,432,678 + SO_2322 0.83 +0.5 2,432,685 + SO_2322 0.84 +0.8 2,432,685 + SO_2322 0.84 +1.4 2,432,685 + SO_2322 0.84 -0.7 2,432,685 + SO_2322 0.84 -3.4 2,432,685 + SO_2322 0.84 +0.1 2,432,685 + SO_2322 0.84 -3.7 2,432,687 - SO_2322 0.84 -0.3 2,432,687 - SO_2322 0.84 +0.8 2,432,692 - SO_2322 0.85 +0.4 2,432,693 - SO_2322 0.85 -0.5 2,432,693 - SO_2322 0.85 -0.9 2,432,693 - SO_2322 0.85 +1.3 2,432,725 + SO_2322 0.89 -1.0 2,432,731 + SO_2322 0.90 -0.1 2,432,732 - SO_2322 0.90 -2.8 2,432,732 - SO_2322 0.90 +0.9 2,432,739 - +0.8 2,432,798 + -1.3 2,432,934 + +0.0 2,432,934 + -0.4 2,432,934 + +1.1 2,432,942 - +0.1 2,432,942 - -0.5 2,432,950 - +0.2 2,432,976 - -2.3 2,432,976 - +2.4 2,432,981 + -2.2 2,432,989 - -1.3 2,433,008 - +4.4 2,433,022 + +1.7 2,433,030 - SO2323 0.10 +0.3 2,433,076 + SO2323 0.12 +0.7 2,433,169 - SO2323 0.17 +0.7 2,433,203 - SO2323 0.19 +0.5 2,433,255 - SO2323 0.22 +2.2 2,433,262 + SO2323 0.22 +0.9 2,433,269 + SO2323 0.22 -0.6 2,433,269 + SO2323 0.22 +0.4 2,433,269 + SO2323 0.22 +0.4 2,433,277 - SO2323 0.23 +1.0 2,433,277 - SO2323 0.23 -0.4 2,433,277 - SO2323 0.23 +1.0 2,433,277 - SO2323 0.23 +0.3 2,433,277 - SO2323 0.23 -1.2 2,433,304 + SO2323 0.24 +0.9 2,433,304 + SO2323 0.24 +0.3 2,433,311 - SO2323 0.24 +0.9 2,433,312 - SO2323 0.25 -1.5 2,433,344 - SO2323 0.26 -0.9 2,433,355 + SO2323 0.27 +1.1 2,433,362 + SO2323 0.27 +2.2 2,433,362 + SO2323 0.27 -0.5 2,433,362 + SO2323 0.27 -1.3 2,433,362 + SO2323 0.27 -3.3 2,433,362 + SO2323 0.27 +0.7 2,433,362 + SO2323 0.27 -1.7 2,433,362 + SO2323 0.27 +0.0 2,433,362 + SO2323 0.27 -0.6 2,433,362 + SO2323 0.27 -3.5 2,433,362 + SO2323 0.27 +0.3 2,433,362 + SO2323 0.27 +1.2 2,433,362 + SO2323 0.27 -3.1 2,433,367 + SO2323 0.27 -0.5 2,433,370 - SO2323 0.28 +0.1 2,433,370 - SO2323 0.28 +1.4 2,433,370 - SO2323 0.28 +0.2 2,433,370 - SO2323 0.28 +1.0 2,433,370 - SO2323 0.28 +1.0 2,433,370 - SO2323 0.28 -2.2 2,433,370 - SO2323 0.28 +1.2 2,433,370 - SO2323 0.28 +1.1 2,433,370 - SO2323 0.28 +2.0 2,433,370 - SO2323 0.28 -0.3 2,433,370 - SO2323 0.28 -1.9 2,433,370 - SO2323 0.28 -0.3 2,433,407 - SO2323 0.29 +2.9 2,433,433 + SO2323 0.31 -0.3 2,433,626 + SO2323 0.41 +2.1 2,433,626 + SO2323 0.41 -0.3 2,433,626 + SO2323 0.41 -0.2 2,433,626 + SO2323 0.41 -0.3 2,433,626 + SO2323 0.41 +0.2 2,433,626 + SO2323 0.41 -1.8 2,433,629 - SO2323 0.41 +0.3 2,433,634 - SO2323 0.41 +2.1 2,433,634 - SO2323 0.41 +1.1 2,433,634 - SO2323 0.41 +1.0 2,433,634 - SO2323 0.41 +0.3 2,433,634 - SO2323 0.41 -0.5 2,433,634 - SO2323 0.41 -0.9 2,433,634 - SO2323 0.41 +2.0 2,433,686 + SO2323 0.44 +0.2 2,433,686 + SO2323 0.44 +0.8 2,433,694 - SO2323 0.44 -0.2 2,433,720 + SO2323 0.46 +0.4 2,433,720 + SO2323 0.46 +2.8 2,433,729 - SO2323 0.46 +1.7 2,433,729 - SO2323 0.46 -1.5 2,433,729 - SO2323 0.46 +0.4 2,433,729 - SO2323 0.46 +1.7 2,433,823 - SO2323 0.51 -0.7 2,433,878 + SO2323 0.54 -0.1 2,433,878 + SO2323 0.54 +0.3 2,433,878 + SO2323 0.54 +1.7 2,433,886 - SO2323 0.54 +0.5 2,433,905 + SO2323 0.55 +1.4 2,433,925 + SO2323 0.56 +0.7 2,433,969 - SO2323 0.58 -2.2 2,433,984 + SO2323 0.59 +0.7 2,433,995 + SO2323 0.60 -2.7 2,434,002 + SO2323 0.60 +1.8 2,434,002 + SO2323 0.60 -1.7 2,434,010 - SO2323 0.60 +1.8 2,434,037 + SO2323 0.62 -0.7 2,434,045 - SO2323 0.62 +1.7 2,434,063 - SO2323 0.63 -0.6 2,434,107 - SO2323 0.65 -0.1 2,434,160 + SO2323 0.68 +0.7 2,434,169 - SO2323 0.69 +2.8 2,434,180 + SO2323 0.69 +1.2 2,434,295 + SO2323 0.75 +1.3 2,434,316 + SO2323 0.76 +1.4 2,434,317 - SO2323 0.76 +1.5 2,434,331 + SO2323 0.77 +1.7 2,434,331 + SO2323 0.77 +1.7 2,434,339 - SO2323 0.77 +0.8 2,434,559 - SO2323 0.89 +1.0 2,434,640 + -0.1 2,434,684 + -1.5 2,434,789 - +0.6 2,434,923 + +0.1 2,434,923 + cheW-2 SO2324 0.25 +0.1 2,435,186 + cheW-2 SO2324 0.74 +1.8 2,435,208 + cheW-2 SO2324 0.78 +0.9 2,435,214 - cheW-2 SO2324 0.79 +0.1 2,435,214 - cheW-2 SO2324 0.79 -0.9 2,435,227 - cheW-2 SO2324 0.81 +2.2 2,435,254 + cheW-2 SO2324 0.86 +0.3 2,435,325 + -2.0 2,435,328 - +1.4 2,435,328 - +2.4 2,435,342 + -0.6 2,435,356 + -0.0 2,435,364 - -0.5 2,435,448 + cheR-3 SO2325 0.15 -0.5 2,435,694 + cheR-3 SO2325 0.45 +0.5 2,435,697 - cheR-3 SO2325 0.45 -3.6 2,435,726 - cheR-3 SO2325 0.49 +0.8 2,435,753 + cheR-3 SO2325 0.52 +0.1 2,435,753 - cheR-3 SO2325 0.52 -0.5 2,435,761 - cheR-3 SO2325 0.53 +1.3 2,435,772 + cheR-3 SO2325 0.54 +1.3
Or see this region's nucleotide sequence