Strain Fitness in Shewanella oneidensis MR-1 around SO2268

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2265 and SO2266 are separated by 14 nucleotidesSO2266 and hscB are separated by 15 nucleotideshscB and hscA are separated by 67 nucleotideshscA and SO2269 are separated by 8 nucleotidesSO2269 and rimK-2 are separated by 155 nucleotides SO2265: SO2265 - NifU family protein (NCBI ptt file), at 2,380,203 to 2,380,586 SO2265 SO2266: SO2266 - HesB/YadR/YfhF family protein (NCBI ptt file), at 2,380,601 to 2,380,924 SO2266 SO2267: hscB - co-chaperone Hsc20 (NCBI ptt file), at 2,380,940 to 2,381,464 hscB SO2268: hscA - chaperone protein HscA (NCBI ptt file), at 2,381,532 to 2,383,394 hscA SO2269: SO2269 - ferredoxin, 2Fe-2S (NCBI ptt file), at 2,383,403 to 2,383,738 SO2269 SO2270: rimK-2 - ribosomal protein S6 modification protein (NCBI ptt file), at 2,383,894 to 2,384,793 rimK-2 Position (kb) 2381 2382 2383 2384Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 2382.817 kb on + strand, within hscAat 2383.844 kb on - strandat 2384.129 kb on + strand, within rimK-2at 2384.283 kb on - strand, within rimK-2at 2384.329 kb on + strand, within rimK-2at 2384.342 kb on - strand, within rimK-2

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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2,382,817 + hscA SO2268 0.69 +4.8
2,383,844 - -1.6
2,384,129 + rimK-2 SO2270 0.26 +0.3
2,384,283 - rimK-2 SO2270 0.43 -0.3
2,384,329 + rimK-2 SO2270 0.48 -2.9
2,384,342 - rimK-2 SO2270 0.50 +0.8

Or see this region's nucleotide sequence