Strain Fitness in Shewanella oneidensis MR-1 around SO2245

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2244 and dnaQ-1 are separated by 402 nucleotidesdnaQ-1 and SO2247 are separated by 402 nucleotides SO2244: SO2244 - transcriptional regulator, LacI family (NCBI ptt file), at 2,359,135 to 2,360,190 SO2244 SO2245: dnaQ-1 - DNA polymerase III, epsilon subunit (NCBI ptt file), at 2,360,593 to 2,361,231 dnaQ-1 SO2247: SO2247 - hypothetical protein (NCBI ptt file), at 2,361,634 to 2,362,371 SO2247 Position (kb) 2360 2361 2362Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2359.641 kb on + strand, within SO2244at 2359.942 kb on - strand, within SO2244at 2359.987 kb on + strand, within SO2244at 2360.027 kb on + strand, within SO2244at 2360.091 kb on + strandat 2360.141 kb on + strandat 2360.141 kb on + strandat 2360.149 kb on - strandat 2360.195 kb on + strandat 2360.342 kb on + strandat 2360.884 kb on - strand, within dnaQ-1at 2361.161 kb on - strand, within dnaQ-1at 2361.351 kb on + strandat 2361.471 kb on + strandat 2361.471 kb on + strandat 2361.479 kb on - strandat 2361.479 kb on - strandat 2361.479 kb on - strandat 2361.479 kb on - strandat 2361.502 kb on + strandat 2361.665 kb on + strandat 2361.680 kb on - strandat 2361.854 kb on - strand, within SO2247at 2361.856 kb on + strand, within SO2247at 2361.864 kb on - strand, within SO2247at 2361.875 kb on + strand, within SO2247at 2362.179 kb on + strand, within SO2247

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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2,359,641 + SO2244 0.48 +0.7
2,359,942 - SO2244 0.76 +1.0
2,359,987 + SO2244 0.81 -0.4
2,360,027 + SO2244 0.84 +2.1
2,360,091 + +1.2
2,360,141 + +1.2
2,360,141 + -1.5
2,360,149 - -2.2
2,360,195 + +1.1
2,360,342 + -1.7
2,360,884 - dnaQ-1 SO2245 0.46 +1.5
2,361,161 - dnaQ-1 SO2245 0.89 -0.4
2,361,351 + +1.0
2,361,471 + -0.9
2,361,471 + +1.0
2,361,479 - +3.0
2,361,479 - +0.5
2,361,479 - -0.9
2,361,479 - +1.0
2,361,502 + -0.5
2,361,665 + -1.0
2,361,680 - +0.6
2,361,854 - SO2247 0.30 +1.0
2,361,856 + SO2247 0.30 -1.9
2,361,864 - SO2247 0.31 +0.6
2,361,875 + SO2247 0.33 +1.0
2,362,179 + SO2247 0.74 +1.7

Or see this region's nucleotide sequence