Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsI and ptsH are separated by 29 nucleotides ptsH and SO2239 are separated by 32 nucleotides SO2239 and SO2240 are separated by 164 nucleotides SO2240 and SO2241 are separated by 345 nucleotides
SO2237: ptsI - phosphoenolpyruvate-protein phosphotransferase (NCBI ptt file), at 2,353,245 to 2,354,948
ptsI
SO2238: ptsH - phosphocarrier protein HPr (NCBI ptt file), at 2,354,978 to 2,355,235
ptsH
SO2239: SO2239 - conserved hypothetical protein (NCBI ptt file), at 2,355,268 to 2,355,654
SO2239
SO2240: SO2240 - methyl-accepting chemotaxis protein (NCBI ptt file), at 2,355,819 to 2,357,456
SO2240
SO2241: SO2241 - conserved hypothetical protein (NCBI ptt file), at 2,357,802 to 2,358,530
SO2241
Position (kb)
2355
2356
2357
2358 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2354.892 kb on + strand at 2354.892 kb on - strand at 2354.900 kb on - strand at 2354.907 kb on - strand at 2354.988 kb on - strand at 2355.196 kb on - strand, within ptsH at 2355.372 kb on - strand at 2355.494 kb on - strand, within SO2239 at 2355.533 kb on - strand, within SO2239 at 2355.535 kb on - strand, within SO2239 at 2355.725 kb on - strand at 2355.725 kb on - strand at 2355.740 kb on + strand at 2355.833 kb on + strand at 2355.846 kb on + strand at 2355.846 kb on + strand at 2355.854 kb on - strand at 2355.881 kb on - strand at 2355.881 kb on - strand at 2355.964 kb on + strand at 2356.121 kb on + strand, within SO2240 at 2356.258 kb on + strand, within SO2240 at 2356.378 kb on + strand, within SO2240 at 2356.386 kb on + strand, within SO2240 at 2356.432 kb on + strand, within SO2240 at 2356.438 kb on - strand, within SO2240 at 2356.589 kb on + strand, within SO2240 at 2356.634 kb on + strand, within SO2240 at 2356.634 kb on + strand, within SO2240 at 2356.634 kb on + strand, within SO2240 at 2356.635 kb on + strand, within SO2240 at 2356.635 kb on + strand, within SO2240 at 2356.642 kb on - strand, within SO2240 at 2356.642 kb on - strand, within SO2240 at 2356.710 kb on - strand, within SO2240 at 2356.742 kb on + strand, within SO2240 at 2356.942 kb on - strand, within SO2240 at 2357.186 kb on - strand, within SO2240 at 2357.196 kb on + strand, within SO2240 at 2357.264 kb on - strand, within SO2240 at 2357.299 kb on - strand at 2357.391 kb on - strand at 2357.493 kb on - strand at 2357.580 kb on - strand at 2357.592 kb on - strand at 2357.603 kb on - strand at 2357.648 kb on - strand at 2357.865 kb on - strand at 2357.868 kb on + strand at 2357.868 kb on + strand at 2357.873 kb on + strand at 2357.876 kb on - strand, within SO2241 at 2357.900 kb on - strand, within SO2241 at 2357.933 kb on - strand, within SO2241 at 2357.944 kb on - strand, within SO2241 at 2357.950 kb on - strand, within SO2241 at 2358.047 kb on + strand, within SO2241 at 2358.055 kb on - strand, within SO2241 at 2358.096 kb on - strand, within SO2241 at 2358.266 kb on - strand, within SO2241
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,354,892 + -2.2 2,354,892 - -4.2 2,354,900 - -3.2 2,354,907 - -3.4 2,354,988 - +1.9 2,355,196 - ptsH SO2238 0.84 -4.1 2,355,372 - +1.1 2,355,494 - SO2239 0.58 +2.6 2,355,533 - SO2239 0.68 +2.6 2,355,535 - SO2239 0.69 +1.1 2,355,725 - +0.7 2,355,725 - +2.1 2,355,740 + +0.5 2,355,833 + +1.0 2,355,846 + +0.7 2,355,846 + -2.2 2,355,854 - -0.9 2,355,881 - -1.2 2,355,881 - -1.6 2,355,964 + -0.9 2,356,121 + SO2240 0.18 -0.1 2,356,258 + SO2240 0.27 +1.5 2,356,378 + SO2240 0.34 +3.1 2,356,386 + SO2240 0.35 +1.6 2,356,432 + SO2240 0.37 +1.0 2,356,438 - SO2240 0.38 +1.6 2,356,589 + SO2240 0.47 +0.8 2,356,634 + SO2240 0.50 +0.1 2,356,634 + SO2240 0.50 +3.1 2,356,634 + SO2240 0.50 +1.5 2,356,635 + SO2240 0.50 +0.1 2,356,635 + SO2240 0.50 +1.5 2,356,642 - SO2240 0.50 -1.0 2,356,642 - SO2240 0.50 +1.1 2,356,710 - SO2240 0.54 +1.3 2,356,742 + SO2240 0.56 +3.6 2,356,942 - SO2240 0.69 -3.3 2,357,186 - SO2240 0.83 -1.9 2,357,196 + SO2240 0.84 +1.7 2,357,264 - SO2240 0.88 -1.5 2,357,299 - +2.3 2,357,391 - +0.7 2,357,493 - +0.5 2,357,580 - +2.0 2,357,592 - +0.6 2,357,603 - +0.9 2,357,648 - -0.4 2,357,865 - -0.5 2,357,868 + -2.0 2,357,868 + +0.6 2,357,873 + +1.2 2,357,876 - SO2241 0.10 +0.3 2,357,900 - SO2241 0.13 +3.4 2,357,933 - SO2241 0.18 +1.3 2,357,944 - SO2241 0.19 -1.4 2,357,950 - SO2241 0.20 -1.4 2,358,047 + SO2241 0.34 +1.0 2,358,055 - SO2241 0.35 +0.1 2,358,096 - SO2241 0.40 -1.7 2,358,266 - SO2241 0.64 +0.0
Or see this region's nucleotide sequence