Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsI and ptsH are separated by 29 nucleotides ptsH and SO2239 are separated by 32 nucleotides SO2239 and SO2240 are separated by 164 nucleotides
SO2237: ptsI - phosphoenolpyruvate-protein phosphotransferase (NCBI ptt file), at 2,353,245 to 2,354,948
ptsI
SO2238: ptsH - phosphocarrier protein HPr (NCBI ptt file), at 2,354,978 to 2,355,235
ptsH
SO2239: SO2239 - conserved hypothetical protein (NCBI ptt file), at 2,355,268 to 2,355,654
SO2239
SO2240: SO2240 - methyl-accepting chemotaxis protein (NCBI ptt file), at 2,355,819 to 2,357,456
SO2240
Position (kb)
2355
2356 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2354.460 kb on + strand, within ptsI at 2354.468 kb on - strand, within ptsI at 2354.660 kb on + strand, within ptsI at 2354.792 kb on + strand at 2354.892 kb on + strand at 2354.892 kb on - strand at 2354.900 kb on - strand at 2354.907 kb on - strand at 2354.988 kb on - strand at 2355.196 kb on - strand, within ptsH at 2355.372 kb on - strand at 2355.494 kb on - strand, within SO2239 at 2355.533 kb on - strand, within SO2239 at 2355.535 kb on - strand, within SO2239 at 2355.725 kb on - strand at 2355.725 kb on - strand at 2355.740 kb on + strand at 2355.833 kb on + strand at 2355.846 kb on + strand at 2355.846 kb on + strand at 2355.854 kb on - strand at 2355.881 kb on - strand at 2355.881 kb on - strand at 2355.964 kb on + strand at 2356.121 kb on + strand, within SO2240 at 2356.258 kb on + strand, within SO2240 at 2356.378 kb on + strand, within SO2240 at 2356.386 kb on + strand, within SO2240 at 2356.432 kb on + strand, within SO2240 at 2356.438 kb on - strand, within SO2240 at 2356.589 kb on + strand, within SO2240 at 2356.634 kb on + strand, within SO2240 at 2356.634 kb on + strand, within SO2240 at 2356.634 kb on + strand, within SO2240 at 2356.635 kb on + strand, within SO2240 at 2356.635 kb on + strand, within SO2240 at 2356.642 kb on - strand, within SO2240 at 2356.642 kb on - strand, within SO2240
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,354,460 + ptsI SO2237 0.71 -1.4 2,354,468 - ptsI SO2237 0.72 -0.8 2,354,660 + ptsI SO2237 0.83 -2.2 2,354,792 + -3.3 2,354,892 + -2.2 2,354,892 - -4.2 2,354,900 - -3.2 2,354,907 - -3.4 2,354,988 - +1.9 2,355,196 - ptsH SO2238 0.84 -4.1 2,355,372 - +1.1 2,355,494 - SO2239 0.58 +2.6 2,355,533 - SO2239 0.68 +2.6 2,355,535 - SO2239 0.69 +1.1 2,355,725 - +0.7 2,355,725 - +2.1 2,355,740 + +0.5 2,355,833 + +1.0 2,355,846 + +0.7 2,355,846 + -2.2 2,355,854 - -0.9 2,355,881 - -1.2 2,355,881 - -1.6 2,355,964 + -0.9 2,356,121 + SO2240 0.18 -0.1 2,356,258 + SO2240 0.27 +1.5 2,356,378 + SO2240 0.34 +3.1 2,356,386 + SO2240 0.35 +1.6 2,356,432 + SO2240 0.37 +1.0 2,356,438 - SO2240 0.38 +1.6 2,356,589 + SO2240 0.47 +0.8 2,356,634 + SO2240 0.50 +0.1 2,356,634 + SO2240 0.50 +3.1 2,356,634 + SO2240 0.50 +1.5 2,356,635 + SO2240 0.50 +0.1 2,356,635 + SO2240 0.50 +1.5 2,356,642 - SO2240 0.50 -1.0 2,356,642 - SO2240 0.50 +1.1
Or see this region's nucleotide sequence