Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2181 and SO2182 are separated by 176 nucleotides SO2182 and SO2183 are separated by 58 nucleotides SO2183 and ppx are separated by 371 nucleotides
SO2181: SO2181 - hypothetical protein (NCBI ptt file), at 2,292,907 to 2,293,191
SO2181
SO2182: SO2182 - conserved hypothetical protein (NCBI ptt file), at 2,293,368 to 2,293,970
SO2182
SO2183: SO2183 - conserved hypothetical protein (NCBI ptt file), at 2,294,029 to 2,294,544
SO2183
SO2185: ppx - exopolyphosphatase (NCBI ptt file), at 2,294,916 to 2,296,472
ppx
Position (kb)
2294
2295 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2293.089 kb on - strand, within SO2181 at 2293.708 kb on + strand, within SO2182 at 2293.708 kb on + strand, within SO2182 at 2293.873 kb on - strand, within SO2182 at 2293.909 kb on + strand, within SO2182 at 2293.943 kb on - strand at 2293.987 kb on + strand at 2294.027 kb on + strand at 2294.203 kb on - strand, within SO2183 at 2294.216 kb on + strand, within SO2183 at 2294.216 kb on + strand, within SO2183 at 2294.298 kb on - strand, within SO2183 at 2294.313 kb on - strand, within SO2183 at 2294.431 kb on - strand, within SO2183 at 2294.460 kb on + strand, within SO2183 at 2294.527 kb on + strand at 2294.527 kb on + strand at 2294.527 kb on + strand at 2294.527 kb on - strand at 2294.535 kb on - strand at 2294.730 kb on - strand at 2294.824 kb on + strand at 2294.824 kb on + strand at 2294.859 kb on + strand at 2294.937 kb on - strand at 2294.976 kb on + strand at 2294.978 kb on - strand at 2295.005 kb on + strand at 2295.083 kb on + strand, within ppx at 2295.202 kb on + strand, within ppx at 2295.202 kb on + strand, within ppx at 2295.352 kb on + strand, within ppx at 2295.394 kb on + strand, within ppx
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,293,089 - SO2181 0.64 +0.2 2,293,708 + SO2182 0.56 -0.6 2,293,708 + SO2182 0.56 +2.5 2,293,873 - SO2182 0.84 -1.5 2,293,909 + SO2182 0.90 +0.1 2,293,943 - +0.2 2,293,987 + -0.5 2,294,027 + -0.4 2,294,203 - SO2183 0.34 -3.4 2,294,216 + SO2183 0.36 -2.9 2,294,216 + SO2183 0.36 -0.7 2,294,298 - SO2183 0.52 -4.6 2,294,313 - SO2183 0.55 -2.4 2,294,431 - SO2183 0.78 -0.3 2,294,460 + SO2183 0.84 -0.4 2,294,527 + -0.7 2,294,527 + -0.2 2,294,527 + -0.6 2,294,527 - -0.8 2,294,535 - +0.2 2,294,730 - -3.3 2,294,824 + -1.3 2,294,824 + +1.6 2,294,859 + +0.1 2,294,937 - +0.1 2,294,976 + -0.4 2,294,978 - +0.3 2,295,005 + -0.2 2,295,083 + ppx SO2185 0.11 -1.5 2,295,202 + ppx SO2185 0.18 -1.2 2,295,202 + ppx SO2185 0.18 +3.9 2,295,352 + ppx SO2185 0.28 -1.3 2,295,394 + ppx SO2185 0.31 +1.0
Or see this region's nucleotide sequence