Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ccpA and SO2179 are separated by 528 nucleotides SO2179 and SO2180 are separated by 67 nucleotides
SO2178: ccpA - cytochrome c551 peroxidase (NCBI ptt file), at 2,289,445 to 2,290,446
ccpA
SO2179: SO2179 - hypothetical protein (NCBI ptt file), at 2,290,975 to 2,291,850
SO2179
SO2180: SO2180 - peptidase, M23/M37 family (NCBI ptt file), at 2,291,918 to 2,292,853
SO2180
Position (kb)
2290
2291
2292 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2290.046 kb on - strand, within ccpA at 2290.136 kb on + strand, within ccpA at 2290.186 kb on + strand, within ccpA at 2290.222 kb on + strand, within ccpA at 2290.640 kb on - strand at 2290.757 kb on + strand at 2291.081 kb on + strand, within SO2179 at 2291.129 kb on + strand, within SO2179 at 2291.437 kb on - strand, within SO2179 at 2291.437 kb on - strand, within SO2179 at 2291.494 kb on - strand, within SO2179 at 2291.535 kb on + strand, within SO2179 at 2291.543 kb on - strand, within SO2179 at 2291.543 kb on - strand, within SO2179 at 2291.608 kb on - strand, within SO2179 at 2291.608 kb on - strand, within SO2179 at 2291.825 kb on + strand at 2291.844 kb on + strand at 2291.844 kb on + strand at 2291.852 kb on - strand at 2291.928 kb on - strand at 2291.941 kb on + strand at 2292.041 kb on + strand, within SO2180 at 2292.043 kb on + strand, within SO2180 at 2292.043 kb on - strand, within SO2180 at 2292.098 kb on + strand, within SO2180 at 2292.106 kb on - strand, within SO2180 at 2292.252 kb on + strand, within SO2180 at 2292.260 kb on + strand, within SO2180 at 2292.373 kb on + strand, within SO2180 at 2292.466 kb on - strand, within SO2180 at 2292.466 kb on - strand, within SO2180 at 2292.466 kb on - strand, within SO2180 at 2292.468 kb on - strand, within SO2180 at 2292.743 kb on + strand, within SO2180 at 2292.743 kb on + strand, within SO2180 at 2292.782 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,290,046 - ccpA SO2178 0.60 -0.9 2,290,136 + ccpA SO2178 0.69 +0.7 2,290,186 + ccpA SO2178 0.74 +0.9 2,290,222 + ccpA SO2178 0.78 -0.9 2,290,640 - -1.9 2,290,757 + +0.3 2,291,081 + SO2179 0.12 +1.3 2,291,129 + SO2179 0.18 +5.0 2,291,437 - SO2179 0.53 +1.1 2,291,437 - SO2179 0.53 -1.8 2,291,494 - SO2179 0.59 +2.5 2,291,535 + SO2179 0.64 -3.7 2,291,543 - SO2179 0.65 -1.1 2,291,543 - SO2179 0.65 +0.3 2,291,608 - SO2179 0.72 -1.5 2,291,608 - SO2179 0.72 +0.5 2,291,825 + +0.8 2,291,844 + -2.7 2,291,844 + -0.1 2,291,852 - -0.2 2,291,928 - -1.5 2,291,941 + -3.0 2,292,041 + SO2180 0.13 -0.5 2,292,043 + SO2180 0.13 +0.8 2,292,043 - SO2180 0.13 -2.5 2,292,098 + SO2180 0.19 +0.7 2,292,106 - SO2180 0.20 -1.5 2,292,252 + SO2180 0.36 +0.1 2,292,260 + SO2180 0.37 +0.7 2,292,373 + SO2180 0.49 -1.2 2,292,466 - SO2180 0.59 +4.0 2,292,466 - SO2180 0.59 -1.4 2,292,466 - SO2180 0.59 -1.6 2,292,468 - SO2180 0.59 -0.8 2,292,743 + SO2180 0.88 +1.8 2,292,743 + SO2180 0.88 +1.1 2,292,782 + -2.2
Or see this region's nucleotide sequence