Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2096 and hydC are separated by 7 nucleotides hydC and hyaB are separated by 21 nucleotides hyaB and hoxK are separated by 3 nucleotides
SO2096: SO2096 - hydrogenase expression/formation protein (NCBI ptt file), at 2,193,880 to 2,194,458
SO2096
SO2097: hydC - quinone-reactive Ni/Fe hydrogenase, cytochrome b subunit (NCBI ptt file), at 2,194,466 to 2,195,140
hydC
SO2098: hyaB - quinone-reactive Ni/Fe hydrogenase, large subunit (NCBI ptt file), at 2,195,162 to 2,196,865
hyaB
SO2099: hoxK - quinone-reactive Ni/Fe hydrogenase, small subunit precursor (NCBI ptt file), at 2,196,869 to 2,198,005
hoxK
Position (kb)
2195
2196
2197 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2194.187 kb on - strand, within SO2096 at 2194.347 kb on + strand, within SO2096 at 2194.347 kb on + strand, within SO2096 at 2194.347 kb on + strand, within SO2096 at 2194.355 kb on - strand, within SO2096 at 2194.540 kb on + strand, within hydC at 2194.657 kb on - strand, within hydC at 2194.657 kb on - strand, within hydC at 2194.679 kb on + strand, within hydC at 2194.679 kb on + strand, within hydC at 2194.741 kb on + strand, within hydC at 2194.749 kb on - strand, within hydC at 2194.749 kb on - strand, within hydC at 2195.061 kb on - strand, within hydC at 2195.068 kb on + strand, within hydC at 2195.224 kb on + strand at 2195.280 kb on - strand at 2195.323 kb on + strand at 2195.468 kb on - strand, within hyaB at 2195.587 kb on - strand, within hyaB at 2195.613 kb on - strand, within hyaB at 2195.614 kb on - strand, within hyaB at 2195.641 kb on - strand, within hyaB at 2195.792 kb on + strand, within hyaB at 2195.800 kb on - strand, within hyaB at 2195.840 kb on + strand, within hyaB at 2195.883 kb on + strand, within hyaB at 2195.891 kb on - strand, within hyaB at 2195.913 kb on - strand, within hyaB at 2196.063 kb on + strand, within hyaB at 2196.112 kb on + strand, within hyaB at 2196.167 kb on + strand, within hyaB at 2196.202 kb on + strand, within hyaB at 2196.280 kb on + strand, within hyaB at 2196.333 kb on - strand, within hyaB at 2196.389 kb on + strand, within hyaB at 2196.401 kb on + strand, within hyaB at 2196.403 kb on + strand, within hyaB at 2196.409 kb on - strand, within hyaB at 2196.409 kb on - strand, within hyaB at 2196.409 kb on - strand, within hyaB at 2196.409 kb on - strand, within hyaB at 2196.411 kb on + strand, within hyaB at 2196.513 kb on + strand, within hyaB at 2196.516 kb on - strand, within hyaB at 2196.517 kb on + strand, within hyaB at 2196.540 kb on - strand, within hyaB at 2196.555 kb on - strand, within hyaB at 2196.577 kb on + strand, within hyaB at 2196.585 kb on - strand, within hyaB at 2196.585 kb on - strand, within hyaB at 2196.686 kb on - strand, within hyaB at 2196.715 kb on + strand at 2196.723 kb on - strand at 2196.736 kb on + strand at 2196.736 kb on + strand at 2196.783 kb on + strand at 2196.832 kb on + strand at 2196.959 kb on + strand at 2196.996 kb on - strand, within hoxK at 2197.039 kb on + strand, within hoxK at 2197.039 kb on + strand, within hoxK at 2197.047 kb on - strand, within hoxK at 2197.093 kb on - strand, within hoxK at 2197.163 kb on + strand, within hoxK at 2197.218 kb on - strand, within hoxK at 2197.390 kb on - strand, within hoxK at 2197.401 kb on + strand, within hoxK at 2197.401 kb on + strand, within hoxK at 2197.401 kb on + strand, within hoxK at 2197.409 kb on - strand, within hoxK at 2197.409 kb on - strand, within hoxK at 2197.409 kb on - strand, within hoxK at 2197.409 kb on - strand, within hoxK at 2197.409 kb on - strand, within hoxK at 2197.416 kb on + strand, within hoxK at 2197.416 kb on + strand, within hoxK at 2197.416 kb on + strand, within hoxK at 2197.416 kb on + strand, within hoxK at 2197.508 kb on + strand, within hoxK at 2197.516 kb on - strand, within hoxK at 2197.775 kb on + strand, within hoxK at 2197.834 kb on + strand, within hoxK
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,194,187 - SO2096 0.53 -3.6 2,194,347 + SO2096 0.81 +0.8 2,194,347 + SO2096 0.81 -3.5 2,194,347 + SO2096 0.81 +0.4 2,194,355 - SO2096 0.82 -2.9 2,194,540 + hydC SO2097 0.11 +0.4 2,194,657 - hydC SO2097 0.28 -1.1 2,194,657 - hydC SO2097 0.28 -3.1 2,194,679 + hydC SO2097 0.32 -0.4 2,194,679 + hydC SO2097 0.32 -3.3 2,194,741 + hydC SO2097 0.41 +0.4 2,194,749 - hydC SO2097 0.42 +1.6 2,194,749 - hydC SO2097 0.42 -1.1 2,195,061 - hydC SO2097 0.88 -1.6 2,195,068 + hydC SO2097 0.89 -0.7 2,195,224 + +2.3 2,195,280 - +1.4 2,195,323 + -0.6 2,195,468 - hyaB SO2098 0.18 -0.2 2,195,587 - hyaB SO2098 0.25 +1.3 2,195,613 - hyaB SO2098 0.26 +0.3 2,195,614 - hyaB SO2098 0.27 -0.4 2,195,641 - hyaB SO2098 0.28 +0.6 2,195,792 + hyaB SO2098 0.37 -0.9 2,195,800 - hyaB SO2098 0.37 +1.1 2,195,840 + hyaB SO2098 0.40 +1.9 2,195,883 + hyaB SO2098 0.42 +3.4 2,195,891 - hyaB SO2098 0.43 +1.2 2,195,913 - hyaB SO2098 0.44 -3.0 2,196,063 + hyaB SO2098 0.53 -2.8 2,196,112 + hyaB SO2098 0.56 +0.6 2,196,167 + hyaB SO2098 0.59 -0.8 2,196,202 + hyaB SO2098 0.61 -1.1 2,196,280 + hyaB SO2098 0.66 +0.6 2,196,333 - hyaB SO2098 0.69 +3.7 2,196,389 + hyaB SO2098 0.72 -0.1 2,196,401 + hyaB SO2098 0.73 -2.4 2,196,403 + hyaB SO2098 0.73 +1.5 2,196,409 - hyaB SO2098 0.73 +0.4 2,196,409 - hyaB SO2098 0.73 +1.2 2,196,409 - hyaB SO2098 0.73 +0.4 2,196,409 - hyaB SO2098 0.73 -0.4 2,196,411 + hyaB SO2098 0.73 +2.7 2,196,513 + hyaB SO2098 0.79 +0.9 2,196,516 - hyaB SO2098 0.79 +0.4 2,196,517 + hyaB SO2098 0.80 +1.8 2,196,540 - hyaB SO2098 0.81 -0.2 2,196,555 - hyaB SO2098 0.82 +1.4 2,196,577 + hyaB SO2098 0.83 -1.1 2,196,585 - hyaB SO2098 0.84 +1.5 2,196,585 - hyaB SO2098 0.84 -0.0 2,196,686 - hyaB SO2098 0.89 -1.4 2,196,715 + -0.9 2,196,723 - +0.6 2,196,736 + -0.9 2,196,736 + +0.2 2,196,783 + -1.0 2,196,832 + +2.5 2,196,959 + -0.6 2,196,996 - hoxK SO2099 0.11 +1.4 2,197,039 + hoxK SO2099 0.15 +0.4 2,197,039 + hoxK SO2099 0.15 -0.5 2,197,047 - hoxK SO2099 0.16 -0.8 2,197,093 - hoxK SO2099 0.20 -1.0 2,197,163 + hoxK SO2099 0.26 -0.4 2,197,218 - hoxK SO2099 0.31 -3.2 2,197,390 - hoxK SO2099 0.46 +1.3 2,197,401 + hoxK SO2099 0.47 +1.0 2,197,401 + hoxK SO2099 0.47 -0.0 2,197,401 + hoxK SO2099 0.47 -0.4 2,197,409 - hoxK SO2099 0.47 -1.0 2,197,409 - hoxK SO2099 0.47 -4.0 2,197,409 - hoxK SO2099 0.47 +0.7 2,197,409 - hoxK SO2099 0.47 +0.9 2,197,409 - hoxK SO2099 0.47 +0.2 2,197,416 + hoxK SO2099 0.48 -0.2 2,197,416 + hoxK SO2099 0.48 -0.4 2,197,416 + hoxK SO2099 0.48 +0.4 2,197,416 + hoxK SO2099 0.48 -2.8 2,197,508 + hoxK SO2099 0.56 +0.7 2,197,516 - hoxK SO2099 0.57 -0.8 2,197,775 + hoxK SO2099 0.80 +0.2 2,197,834 + hoxK SO2099 0.85 -0.4
Or see this region's nucleotide sequence